Biomedical subjects

J Tománek

Publications and source records attributed to J Tománek.

14 recordsLinked to original sources

PubMed1986

[Diagnosis of infectious pancreatic necrosis (IPN) of trout using immunofluorescence].

Until recently, demonstration of infectious pancreatic necrosis was conditioned by the isolation of virus. By means of the immunofluorescence method it is possible to make a rapid and accurate diagnosis, as well as to determine the development of infection. In acute cases, the virus in fry was demonstrated mainly in the cytoplasm of epithelial cells of guts; in Pd1-2 trout, the virus was proved in the cytoplasm of epithelial cells of primary kidney tubules and in the cytoplasm of epithelial cells of pancreatic acini. In all cases the virus occurrence demonstrated by immunofluorescence was in correlation with the results achieved by cultivation methods.

Animals↗

PubMed1986

[Isolation and identification of the infectious pancreatic necrosis virus in trout].

The first isolation and identification of the infectious pancreatic necrosis virus in trout in Czechoslovakia is described. The RTG-2, PG and CHSE cell lines were used for isolation and the identification was made by the methods of electron microscopy, cross virus-neutralizing test, and immunofluorescence. As demonstrated, all the isolates in the Czechoslovak territory are serologically identical and are closely related (or perhaps congruent) with the Sp reference strain which has the highest frequency in Europe. The described methodical procedure is also applicable to the diagnostics of other virus diseases of fish.

Animals↗

PubMed1986

[Immunoenzyme (ELISA) diagnosis of spring viremia in carp].

The enzymoimmunologic (ELISA) method was used to diagnose spring viremia in carp. The specific antibodies to Rhabdovirus carpio in the fish sera were detected by this method. Reaction specificity was tested by the use of viral and control antigens, by examination of positive and negative sera and by serum-free control incubation. Applying the ELISA method, the antiviral antibodies can be detected after infection or vaccination: this method is therefore suitable for thorough studies and when choosing the appropriate treatments and preventive measures.

Animals↗

PubMed1983

[Gill structure in healthy carp].

When the gills of carp are studied, this organ should be evaluated from there planes of projection: sagittal, transversal and horizontal. Lamellae, the functional base of the gills, provide the exchange of oxygen between blood and water. Lamellae consist of a network of sinuses with a skeleton formed by columnar cells and with walls constituted by the cytoplasmic processes of these cells. On the outer side the sinuses are covered with one or two layers of flat epithelial cells. The gill filaments and gill arches, the structure and mobility of which enable the perfect function of lamellae are covered with stratified epithelium. Cylindrical cells (germ cells) of this epithelium are borne by a basal membrane; during maturation they flatten and differentiate towards the surface. Besides the already flat epithelial cells, the surface of epithelium also carries mucous cells and gustatory buds. Separate large irregular cells with excentric nucleus and small eosinophilically staining granules are also found in the epithelial layers of healthy fish.

Animals↗

PubMed1983

[Necrotic processes in the gill tissue of carp caused by changes in the aquatic environment].

Histological findings show that various pathogenic processes are involved in the cases of clinically detected branchionecrosis. The changes in gills occurring at toxic branchionecrosis are a necrobiotic process in their nature; in gill lamellae this process manifests itself as a swelling of the respiratory epithelium and as dilation of lamellar sinuses, and in stratified epithelium of gill arch and filaments it takes on the form of the hypertrophy and hyperplasia of epithelial cells. In severe cases the swollen epithelium forms deposits filling up also the interlamellar space. Toxic branchionecrosis is a term referring to a disease when the pathological processes are caused by ammonia intoxication and autointoxication, the main role being played by pH value. At experimental ammonia intoxication the changes in gills are about the same as in toxic branchionecrosis but the symptoms are not so pronounced. Changes characteristic of spherosporosis involve severe necrotic processes in the respiratory epithelium as well as in the stratified epithelium of gills, and the epithelium harbours different developmental stages of spherospores. The spherospores are easily detected when stained with alcian blue at pH 2.5. The changes in the gills of carp naturally invaded by spherospores with additional ammonia intoxication correspond to findings characteristic of toxic branchionecrosis: degeneration of stratified and respiratory epithelium of gills combined with necrotic changes caused by the developmental stages of spherospores. When water is highly oxygenated with chlorinated lime, large cells with eosinophilic granules severely multiply mainly in the stratified epithelium of gills; this process is considered as an inflammatory process. Parasitic invasions by Trichodina and Chilodonella induce local inflammatory reactions with the swelling of the respiratory epithelium.

Ammonia↗

PubMed1981

[Residual larval effects of permethrin and resistance in the housefly].

The effectiveness of synthetic pyrethroid permethrin in a special preparation Coopex, concentrations 0.25 and 0.5% against larval stages of housefly (Musca domestica) was tested. No larvicidal effect was proved in these commonly used concentrations. Under laboratory conditions, a very good residual effectiveness against imagoes, lasting more than 365 days (100% imago perish), was proved. In stables the residual effectiveness of Coopex was pronounced during 11 weeks. In ten generations of housefly, exposed to 0.001% concentration of Coopex, no resistance to 0.25% concentration of this substance was observed.

Animals↗

PubMed1977