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J Trojan

Publications and source records attributed to J Trojan.

At least 91 records · Page 5Linked to original sources
PubMed1981

Localisation of alphafetoprotein (AFP) in murine teratocarcinoma.

Solid tumours with histopathological characteristics of teratocarcinoma (generally presenting the structures of nervous origin) were induced in the mouse by subcutaneous inoculation with murine embryonal carcinoma (EC) cells of line PCC4-azal. An indirect immunoperoxidase technique using anti-mouse AFP antibodies was applied for the localization of the protein on paraffin sections of tissue fixed with acetic acid-ethanol. The PCC4-azal EC cells were AFP negative in vitro. In the solid tumours, the immature embryonal or fetal components (i. e. tubes or cysts mimicking the development of the neural tube) and the groups of neuroepithelial cells arranged in more or less differentiating structures (pseudotubes, cluters) were AFP positive; on the contrary, cells of the same differentiated neuroepithelial type, but lacking recognizable tissue architecture, were AFP negative. Mature, adult components were AFP negative. Undifferentiated cancer components, whether structured or not, were AFP negative. These results suggest that the intracellular presence of AFP requires a minimum degree of both cell differentiation and tissue organization.

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PubMed1980

Immunocytochemical localization of alpha-fetoprotein in the developing rat brain.

The immunocytochemical localization of the alpha-fetoprotein (AFP), serum albumin (ALB), transferrin (Tf) and immunoglobulins (IgG) in developing rat brain has been studied by the indirect peroxidase technique using rabbit antibodies to AFP, ALB, Tf and IgG followed by goat anti-rabbit immunoglobulins conjugated with peroxidase. Evidence of a high selective staining for AFP and ALB is presented. The pattern of morphological localization of both proteins was similar throughout fetal and post-natal development up to 20-25 days of age. Large areas and groups of cells in all regions of the brain from the olfactory bulb to the medulla oblongata appeared positively stained at a given moment in the development. The labeling was cytoplasmic and, in the neuronal elements, extended to their axonic and dentritic prolongations. The intracellular localization of AFP and ALB in the developing rat brain may be related to the binding affinity of these proteins for oestrogens and/or essential fatty acids. The morphological data presented here suggest that both proteins are actively involved in the uptake of such substances by the cellular structures of the brain.

Aging↗
PubMed1979

[Intracellular localization of alpha-fetoprotein and serum albumin in the central nervous system of the rat during fetal and postnatal development].

The morphological localisation of alphafetoprotein (AFP), serumalbumin (SA), transferrin and immunoglobulins (IgG) has been studied in the developing central nervous system of the Rat by immunocytochemical methods. Evidence is presented of a highly selective staining for AFP and SA, both proteins exhibiting the same topographical distribution. Practically all the areas of the brain and the spinal cord are stained at a given moment of the developmental process. The labeling is cytoplasmic and in the neuronal elements extends to their axonic and dendritic prolongations. The localization of AFP and SA in the nervous system may be related to the well known binding properties of these proteins for varied substances (estrogen and/or fatty acids). The morphological data presented here suggest that both proteins may be actively involved in the uptake of such substances by the cellular structures of the nervous tissue.

Aging↗
PubMedDate not supplied

In vitro uptake of exogenous alpha-fetoprotein by chicken dorsal root ganglia.

Exogenous chicken alpha-Fetoprotein (AFP) was added to embryonic chick dorsal root ganglia plated on gelatin-coated tissue culture dishes at different stages during the differentiation process and its intracellular uptake demonstrated by immunocytochemical methods. Other embryonic serum proteins were added as a control. Morphologically well-differentiated neurons (ganglion cells) appeared positively labeled for AFP, contrasting with weakly stained other cell types: these included spindle-shaped cells identified as Schwann cells and larger, often closely packed cells, which could be less mature neurons. Fibroblasts were found negative or faintly stained. No AFP was noticeable in cultures grown in the absence of the protein. These results suggest that the presence of AFP in the developing CNS is for the most part, if not entirely, due to protein uptake as opposed to in situ synthesis.

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PubMedDate not supplied

Immunocytochemical quantitative study of alpha-fetoprotein in normal and neoplastic neural development.

The presence of alpha-fetoprotein (AFP) was studied in developing nervous tissues: rat cerebral cortex and neuroepithelial derivatives of a murine teratocarcinoma. AFP was localized by an indirect immunohistoperoxidase technique. Using serial dilutions of anti-AFP antibodies, the end-points of staining (minimal concentration of antibodies giving a positive labelling) were established. These points enabled the evaluation of the amount of stained AFP at different periods of development. In rat cerebral cortex, AFP showed one maximum amount on the 19th-20th day 'postcoitum' (in moderately differentiated structures), and two minima on the 13th-14th day (in primitively differentiated ones) and the 28th-29th day (in well-differentiated ones), respectively. Similarly, one maximum and two minima were established in the derivatives of teratocarcinoma imitating normal neural development.

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