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Biomedical subjects

J Ueno

Publications and source records attributed to J Ueno.

34 records · Page 2Linked to original sources
PubMed1991

Ultrasonographic appearance of the endometrium in natural and stimulated in-vitro fertilization cycles and its correlation with outcome.

Ultrasonographic evaluation of the endometrium in 56 IVF patients was performed prospectively. Endometrial thickness and pattern were analysed in 18 natural-cycle and 38 stimulated-cycle patients. Thickness was measured from the echogenic interface of the endometrium-myometrium junction on a transverse section at the level of the fundus. Patterns were classified as A (homogeneous, hyperechogenic), B (mixed, with an outer hyperechogenic and inner hypoechogenic layer) or C (fluid-filled cavum with ring configuration). The thickness and pattern distributions were similar in natural- and stimulated-cycle patients. There was no correlation between thickness and serum oestradiol levels, the diameter of the largest follicle or the pregnancy outcome in either group. However, the occurrence of endometrial pattern A on the day prior to oocyte retrieval had a predictive value of 100% for a non-conceptional cycle. In contrast, pattern B occurred in a significantly greater proportion of pregnant than non-pregnant patients.

Adult↗
PubMed1990

[Atrioventricular delay and diastolic mitral regurgitation in patient with DDD pacemaker implantation, and cardiac function].

We examined the mitral valve motion, the mitral valve flow pattern, and changing atrioventricular (AV) delay in 34 patients with DDD pacemaker implantation, using M-mode echocardiography and pulse Doppler echocardiography. In 28 patients, B-B' step and diastolic mitral regurgitation (DMR) were induced by extending the AV delay. 8 of 12 patients who showed B-B' step and DMR with the AV delay less than or equal to 165 mS had heart failures. In such patients, P-B-B' step (DMR) interval was 174 +/- 31 mS shorter than the other patients (216 +/- 26 mS, p less than 0.001), and Q-C interval was 131 +/- 26 mS longer than the other patients (63 +/- 31 mS, p less than 0.001). The minimal value of the AV delay that induces B-B' step and DMR can be decided under the influence of cardiac functions, and this echocardiographic examination is useful, giving us information about the cardiac function of the patients with DDD-PM. B-B' step is the mitral valve motion that corresponds with the DMR, so we can check the DMR through the B-B' step easily.

Aged↗
PubMed1989

[Usefulness of dual-energy digital radiography in detecting atlas and axis].

Cranial dual-energy digital radiography was applied to three patients. The anatomy of the atlas and axis was clearly recognized without utilizing the open-mouth view. We consider it a very effective technique when applied to the patient who experiences difficulty in opening the mouth, like severe traumatic or infantile patients. In addition, it is a useful examination for patients with suspected anomalies of cranio-vertebral junction.

Axis, Cervical Vertebra↗
PubMed1989

[Measurement of anti-HTLV-I antibody titer in cerebrospinal fluid].

Anti HTLV-I antibody was measured in 590 cerebrospinal fluids (CSFs) employing a gelatin particle agglutination (PA) method. Anti HTLV-I antibody was detected in the CSFs from 24 patients (4.1%). The serum from the 24 patients also showed positive results. Out of the 24 patients, 11 cases (45.8%) had HAMs. Of the remaining patients 3 had ATLs with meningeal infiltration. Only 11 cases out of 24 patients were positive for anti HTLV-I antibodies when an immunofluorescence (IF) method was employed. This discrepancy of the results obtained by two methods may be explained by the presence of the patients whose CSFs contain anti HTLV-I antibodies of low titer. It is concluded that PA method is both sensitive and specific for the detection of anti HTLV-I antibodies in CSFs. Further, PA method is more convenient than IF method. So, it will be a very useful tool in the diagnosis of HTLV-I related neurological disorders and asymptomatic carriers.

Adolescent↗
PubMed1986

Brugia pahangi: stage-specific antigens on microfilariae detected by serum from infected jirds or by monoclonal antibodies.

Experiments were carried out to determine whether there are stage-specific antigens on microfilariae of Brugia pahangi, using sera from Mongolian jirds infected with B. pahangi and monoclonal antibodies against microfilariae of B. pahangi. These studies showed that microfilariae have both stage-specific and nonspecific antigens. The nonspecific antigens were also present on adult worms and on infective larvae. Among monoclonal antibodies, 6 out of 14 clones produced antibodies against the microfilarial stage-specific antigens, and 8 clones produced antibodies against nonspecific antigens. These monoclonal antibodies could not distinguish between adults, microfilariae, or infective larvae of B. malayi and B. pahangi.

Animals↗
PubMed1986

The effect of immunoglobulin G1 structure on macrophage binding to supported planar lipid monolayers.

We have studied the antibody-dependent binding of macrophages to supported planar lipid monolayers containing haptenated phospholipids (Tnp-Cap-DPPE). Eight monoclonal anti-TNP IgG1s, which had similar affinities to the TNP residues in solution and in the membranes, were used in the experiment. The results showed that mouse macrophages (P388D1 and J774.1) bound with different affinities to these IgG1-coated lipid monolayers. The monoclonal antibody shown to be deficient in macrophage binding was also relatively ineffective in activating complement. These results indicated that individual monoclonal antibodies of a given subclass may prove deficient in terms of the biological activities associated with the group as a whole.

Animals↗
PubMed1985

Effect of priming with antigen on the expression of C3 receptors on murine B cells and the responses to TI-2 antigens.

The effect of priming with DNP-Ficoll on the expression of receptors for the third component of complement (CR) on B cells was studied. B cells from mice primed with antigen were separated into CR- and CR+ populations by rosetting and density gradient centrifugation, and they were examined for antibody responses to TI-2 antigens in vitro. CR- B cells in spleen cells from mice primed with antigen responded well in contrast with the low responsiveness of CR- B cells from unprimed mice. These CR- B cells from primed mice reached their peak responses 1 day earlier than CR+ B cells from primed or unprimed mice. The response of these CR- B cells was specific for the hapten used for priming, and the priming effect was most conspicuous in spleen cells from mice primed with antigen 3 days previously, and it decreased thereafter. The CR- B cells from primed mice also showed better responses than CR+ B cells to all polyclonal B cell activators (PBA) tested. When CR+ B cells were primed in vitro with DNP-Ficoll for 3 days, the major cell population responsive to the antigen in secondary culture was found to be CR- B cells. On the other hand, CR- B cells in spleens could not respond to DNP-Ficoll challenge after in vitro priming with the antigen for 3 days. These results indicate that B cells lose CR by priming with antigen, although they retain their responsiveness to antigens and PBA. The role of CR on B cells in the antibody response is discussed.

Animals↗
PubMedDate not supplied

Pirarubicin might partly circumvent the P-glycoprotein-mediated drug resistance of human breast cancer tissues.

BACKGROUND: Anthracyclines are the first line antitumor agents against breast cancer, and P-glycoprotein (Pgp) is thought to be the main resistance mechanism against these agents. We have evaluated the chemosensitivity of fresh surgical specimens of breast cancer and compared them with their Pgp-expression. MATERIALS AND METHODS: The in vitro chemosensitivity of 65 surgical specimens obtained from 63 patients with advanced breast cancer was assessed by the histoculture drug response assay (HDRA) using doxorubicin (DXR), pirarubicin [(2"R)-4'-tetrahydropyranyladriamycin: THP], and epirubicin (EPIR). Breast cancer tissues were plated onto collagen gel matrix and incubated with 15 micrograms of DXR or EPIR, or 17 micrograms of THP per ml for 7 days with MTT assessed at the endpoint. The efficacy of the agents was evaluated by the inhibition index (I.I.) of the optical density detected by ELISA reader. RESULTS: When 60% or more I.I. was regarded as in vitro sensitive at each cut-off concentration of the drugs, the overall efficacy rates were 60.7%, 48.6%, and 78.6% for DXR, EPIR, and THP, respectively. Fifty-one surgical specimens were evaluated for the immunohistochemical analysis of Pgp and the correlation between the sensitivity to anthracyclines and the expression of Pgp was compared. Pgp was expressed in 23.5% (12/51) specimens and the efficacy of anthracyclines was reduced in Pgp-positive breast cancer tissues, although this reduction was low in THP with a statistically significant difference when comparing with DXR and EPIR. CONCLUSION: The present results suggest that THP might partly circumvent the mdr1/PgP-mediated drug resistance mechanism in human breast cancer tissue and would have some different antitumor spectra on breast cancer comparing with DXR and EPIR.

ATP Binding Cassette Transporter, Subfamily B, Mem↗