Biomedical subjects

J Uhrová

Publications and source records attributed to J Uhrová.

5 recordsLinked to original sources

PubMed2005

Advanced glycoxidation end products in patients with multiple sclerosis.

Advanced glycoxidation end products (AGEs) play an important role in the pathogenesis of neurodegenerations and we studied if AGEs could represent a useful marker in patients with multiple sclerosis (MS). AGE-products were assessed in cerebrospinal fluid (CSF) and serum of 31 patients with MS and 8 controls. We did not find any statistically significant differences in patients with MS and controls either in CSF or in serum. We have observed a significant association between pentosidine and total AGEs as well as a relationship of both to the protein content in CSF in MS patients. Despite of the involvement of both oxidative stress and RAGE (receptor for AGEs) in the pathogenesis of MS and its experimental model, neither pentosidine nor total AGE were shown as useful markers in this indication. Other compounds and ligands of RAGE are probably of higher significance in MS.

Adult↗

PubMed2004

[An importance of vitamin D metabolites assessment in patients with impaired renal function].

The goal of this prospective multicentric study was to assess concentrations of vitamin D metabolites in patients with renal insufficiency and to monitor response of calcium phosphate metabolism parameters to a focused individualised therapy. The sample consisted of 184 patients, 66 of them were undergoing regular dialysis (Ccreat 0.11 +/- 0.05 ml/sec., age 57.6 +/- 16.6) and 118 patients were dispensed for renal insufficiency (Ccreat 0.42 +/- 0.23 ml/sec., age 60.8 +/- 11.0). After an assessment of basic parameters of bone metabolism (Ca, Pi, ALP) and parameters of acidobasic balance, calcidiol, calcitriol, and parathormon were assessed by radiation immunisation and than the used treatment was evaluated and adjusted according to results of the assessment. Two month later laboratory tests were done. Entry concentrations of calcidiol were in 73% of patients in reference area. However, according to clinical recommendations the bottom value of the reference area had to be reevaluated towards higher values. Such more strict criteria suited only 20% of patients. Calcitriol levels in reference area were found in 30% of patients, after treatment adjustment in 49% of patients. Treatment with vitamin D pharmaceuticals was often limited by hyperphosphataemia, low PTH or hypercalinemia. Input levels of calcitriol in nondialised patients significantly correlated with input calcidiol, 1alpha-hydroxylasis in kidneys could be stimulated in them via calcidiol administration. Attention deserve especially low calcidiol and calcitriol levels in patients with renal failure because timely, individualised and controllable supplementation of vitamin D metabolites in renal insufficiency serves as a prevention of later advanced forms of bone metabolism impairment in a period of dialysis treatment.

Calcifediol↗

PubMed2003

[Parameters of bone metabolism in patients with various degrees of kidney function damage].

BACKGROUND: Patients with renal failure frequently have their calcium and phosphate metabolism seriously disrupted. It may result in a skeletal malady--the renal osteopathy. Late forms of this syndrome are difficult to cure. The aim of this comparative study is to follow the relation between parameters of the bone metabolism (calcitriol, calcidiol, parathormone, calcitonin, osteocalcin, Pi, Ca--total or ionised, and others) and the degree of deterioration of the kidney function. METHODS AND RESULTS: Three groups of patients were included into the study: A-hemodialyzed patients with chronic renal failure (Ccreat = 0.07 +/- 0.02 ml/s, n = 21, age 71.0 +/- 10.6 years); B--not dialyzed patients with decreased renal function (Ccreat = 0.33 +/- 0.05 ml/s, n = 19, age 65.0 +/- 9.6 years); C--patients with normal renal function (Ccreat = 1.45 +/- 0.12 ml/s, n = 16, age 85.2 +/- 4.7 years). Calcidiol concentration [microgram/l] did not differ in individual groups (A: 11.3 +/- 4.7, B: 10.7 +/- 8.2, C: 11.7 +/- 5.7, reference limits RM: 8.9-46.7). In contrast, calcitriol concentration [ng/l] was statistically different in all studied groups (A: 1.7 +/- 2.8, B: 17.6 +/- 12.4, C: 30.6 +/- 9.1, p < 0.001, RM: 19.9-67.0) and it correlated with the degree of renal function deterioration (calcitriol vs. creatinine, r = -0.76, p < 0.001). In PTH levels (pmol/l) the group C differed significantly from groups A and B (A: 27.4 +/- 32.0, B: 23.7 +/- 16.5, C: 6.2 +/- 2.4, C vs. A, p < 0.01, C vs. B, p < 0.001, RM: 1.0-6.8). PTH concentrations correlated with osteocalcine and HCO3 (r = 0.74, r = -0.56, p < 0.001). CONCLUSIONS: Results of the tested parameters have shown that abnormalities in the bone metabolism significantly correlate with the degree of renal deterioration. It demonstrates the requirements for vitamin D metabolites supplementation for patients is needed already in the pre-dialysis stage.

Aged↗

PubMed2001

[Freely programmable automatic radioimmunoanalysis on the STRATEC ST 300 analyzer].

We compared the free programmable automatic radioimmunoassay on analyser STRATEC SR 300 to the manual performance of RIA and IRMA. One-step and two-step methods were evaluated. Manual delivery proved to be faster than the automatic one. The speed of dispensing depends on the volume of the dose sample and of the reagent, on the adjustment of rinsing volumes of the needle, washing volumes among separate doses and also on the size of the sample set. The total efficiency of the manual and the automatic analysis is also affected by other operations, especially by washing test tubes and the administration of reports. For a set of 100 analyses, the manual process is from 20 to 90 minutes longer than the automatic one, depending on the analytical method used. Thus, the automatic analysis proved to be significantly faster. Nor the comparison of isotope and non-isotope immunoassays showed any relevant qualitative or quantitative differences in analytical response. At the same time the prices of radioimmunoassays are more favourable, and in addition to that, a big choice of analytical sets is available which are not dependent on the user's instrumentation. However, this does not concern very small series, or separate tests where size of calibration is important. Moreover, the turn-around-time of RIA and IRMA is in these cases longer than when automatic non-isotope methods are used.

Efficiency↗

PubMed1994

Can chemiluminescent immunoanalysis of thyroid hormones stand for a reference method?

Some analytical properties of chemiluminescent immunoassays (ChLIA) for the estimation of total triiodothyronine (T3), total thyroxine (T4) and thyrotropine (TSH) in serum were studied and compared with radioimmunoanalysis (RIA) as a reference method. Measurement range of ChLIA for T3 is lower, for T4 is equivalent and for TSH is greater than by RIA methods. Analytical sensitivity of ChLIA is better for all three analytes. Also precision of ChLIA is much better. ChLIA method seems to be more resistant to lipaemia. When compared to secondary reference materials, i.e. to commercial control sera, higher accuracy can be evaluated. However, adjustment of the control sera and analytical methods to different primary standards and calibrators, resulting in disagreement of results with asigned values, seems to be evident. With respect to the accuracy of ChLIA further study should be performed involving primary standards. Other interferences except of lipaemia, which we refer to in our work, and problems of specificity also need to be elucidated.

Blood Chemical Analysis↗