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J Vácha

Publications and source records attributed to J Vácha.

At least 91 records · Page 5Linked to original sources
PubMed1978

Determination of heme and non-heme iron content of mouse erythropoietic organs.

A procedure is described for determining the content of heme and non-heme iron in organs and tissues of the mouse. Heme iron after homogenization and hemolysis of the samples is extracted as hemin by means of a mixture of ethyl acetate and glacial acetic acid, the extraction being followed by washing with hydrochloric acid. Total iron content is determined with commercial analytical sets exploiting color reaction with bathophenanthroline after mineralization of the samples. The presence of bone or other tissues does not distort the results. The procedure is relatively simple and suitable for serial analyses. The results obtained for the mouse by this method are in good agreement with recognized principles of ferrokinetics in mammals.

Animals↗
PubMed1977

Decreased utilization of [2-14C]orotic acid for the synthesis of cytidine nucleotides in rat liver after administration of alpha-hexachlorocyclohexane.

Administration of alpha-1,2,3,4,5,6-hexachlorocyclohexane (alpha-HCH) to rats decreased the utilization of [2-14C]orotic acid for the synthesis of liver cytidine nucleotides. The specific radioactivities of uridine components of the acid-soluble pool and rRNA increased during the first hours of treatment with the drug. Later on the specific radioactivities of uridine nucleotides remained unchanged, while those of cytidine components decreased gradually. Administration of hydrocortisone increased the incorporation of labelled orotic acid into rRNA cytidylic acid.

Animals↗
PubMed1975

Studies on non-haemoglobin erythrocyte iron; the influence of haemolysis on plasma iron determinations.

Approximately 2% of iron contained in mouse erythrocytes is transferred into supernatant when haemolysed erythrocytes are precipitated with trichloroacetic acid. The component unprecipitable with trichloroacetic acid is probably bound predominantly to reticulocytes and it is larger the younger is the reticulocyte. Under the conditions of postirradiation suppression of erythropoiesis this component grows strongly and in the phase of overrecovery of erythropoiesis decreases. The numerical values determined in the paper can be used for a correction of the disturbing influence of haemolysis on concentration and radioactivity of plasma iron in the resting state of erythropoiesis, if a preliminary precipitation of a sample with trichloroacetic acid was carried out.

Animals↗
PubMed1975

Blood volume in inbred strain BALB/c, CBA/J and C57BL/10 mice determined by means of 59Fe-labelled red cells and 59Fe bound to transferrin.

Circulating red blood cell (RBC) and plasma volume was determined in male inbred strain BALB/c, CBA/J and C57BL/10 mice by parallel use of the 59Fe-labelled RBC dilution and the dilution of 59Fe bound to transferrin. The whole blood volumes values derived from the venous haematocrit and plasma volume were about double the values calculated from the venous haematocrit and circulating RBC volume. Comparison of the two methods thus explains the marked differences in different studies of blood volume in mice and shows that correct values can be obtained only by parallel measurements of RBC and plasma volume by separate methods, or by correcting the venous haematocrit to whole body haematocrit. Combination of the labelled RBC method and the 59Fe-transferrin method showed the blood volume values in the above strains of mice to be 10.35 +/- 0.16, 7.32 +/- 0.10 and 7.94 +/- 0.15 ml/g b.w. respectively. The ratio of whole body to venous haematocrit in these strains was was 57.3 +/- 1.6%, 68.0 +/- 1.8% and 69.5 +/- 2.2%. Significant interstrain differences were demonstrated in RBC, plasma and blood volume and in the venous and whole body haematocrit and their ratio.

Animals↗