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J Vandenhaute

Publications and source records attributed to J Vandenhaute.

4 recordsLinked to original sources
PubMed1991

Cloning and sequencing of the inulinase gene of Kluyveromyces marxianus var. marxianus ATCC 12424.

Cell wall inulinase (EC 3.2.1.7) was purified from Kluyveromyces marxianus var. marxianus (formerly K. fragilis) and its N-terminal 33-amino acid sequence was established. PCR amplification of cDNA with 2 sets of degenerate primers yielded a genomic probe which was then used to screen a genomic library established in the YEp351 yeast shuttle vector. One of the selected recombinant plasmids allowed an invertase-negative Saccharomyces cerevisiae mutant to grow on inulin. It was shown to contain an inulinase gene (INU 1) encoding a 555-amino acid precursor protein with a typical N-terminal signal peptide. The sequence of inulinase displays a high similarity (67%) to S. cerevisiae invertase, suggesting a common evolutionary origin for yeast beta-fructosidases with different substrate preferences.

Amino Acid Sequence↗
PubMed1983

Age-related functional alteration of mouse liver ribosomes.

The in vitro functional capacity of the mouse liver protein synthesis machinery was studied as a function of age. Polysomes from young (one-three months old) and old (18-24 months old) C57BL/6J mice were incubated under standard conditions in a ribosome-free reticulocyte lysate containing [3H]-leucine. The incorporation of radioactivity into hot TCA-insoluble material was measured as a function of time and kinetic curves were compared. A drastic age-related decrease in the initial rate of leucine incorporation was observed when the total ribosomal fraction (containing the whole range of ribosomal aggregates including subunits and single ribosomes) was assayed. When "heavy polysomes" (fractions from which subunits and single ribosomes had been excluded) were compared, the same difference was observed. This latter result indicated that the observed alteration may be attributed to actively translating ribosomes. Results from experiments using inhibitors of initiation suggest that the observed age-related alteration can be attributed to a reduced capacity of ribosomes from older animals to sustain reinitiation.

Aging↗