Biomedical subjects

J Vautrin

Publications and source records attributed to J Vautrin.

29 records · Page 2Linked to original sources

PubMed1991

Characteristics of slow-miniature endplate currents show a subunit composition.

The normal neuromuscular junction shows two classes of spontaneous miniature endplate potentials. These classes are based on a discontinuity in the profile of miniature endplate potential amplitude distributions. The amplitude of one class of miniature endplate potentials from a bell-shaped amplitude distribution and the remaining miniature endplate potentials compose a population which forms a left-hand skew distribution with a mode 1/7 to 1/10 that of the bell-miniature endplate potentials [Kriebel M. E. and Gross C. E. (1974) J. gen. Physiol, 64, 85-103]. Some skew-miniature endplate potentials have a slow time-to-peak and show breaks on the rising phase. Most treatments that alter the miniature endplate potential frequency change the ratio of skew-miniature endplate potentials/bell-miniature endplate potentials [Kriebel M. E. et al. (1976) J. Physiol. 262, 553-581]. The time characteristics of miniature endplate currents were readily altered in the isolated frog and mouse neuromuscular junctions with several agents known to increase the percentage of slow-miniature endplate potentials (heat, botulinum toxin, 4-aminoquinoline and increases in bath osmolarity). The slow-miniature endplate potential amplitudes were a continuum of amplitudes from skew- to giant miniature endplate potentials. The rising phases of miniature endplate potentials were a continuum from smooth to many with breaks and offsets. In a series of sequentially recorded slow-miniature endplate currents, many had congruent rising phases of constant slope regardless of amplitude or of time-to-peak. The rising phases of congruent slow-miniature endplate currents which showed a change in slope deviated at similar amplitudes. The least value of the slope of a slow-miniature endplate current was that of the sub-miniature endplate current; and, miniature endplate currents with overall lower slope values showed a wave pattern and/or irregular breaks which suggests summation of sequentially delayed sub-miniature endplate currents. Plots of the amplitude vs time-to-peak of miniature endplate currents from identified junctions demonstrated that the normal percentage of slow-miniature endplate currents was greatly increased with the treatments used here and that the time-to-peak of giant miniature endplate currents usually was longer than that of normally occurring bell-miniature endplate currents. Giant miniature endplate currents with short time-to-peak values are probably from two miniature endplate currents occurring, by chance, almost simultaneously. During and/or after treatments, miniature endplate currents formed clusters of similar size miniature endplate currents, not randomly distributed in time, which graded from distinct miniature endplate currents to giant miniature endplate currents.(ABSTRACT TRUNCATED AT 400 WORDS)

Action Potentials↗

PubMed1989

Synaptic current between neuromuscular junction folds.

Measurements of membrane infoldings of vertebrate subsynaptic membranes were taken to evaluate the possible electrophysiological implications. The shapes of standard interfolds of different neuromuscular junctions were established from micrographs available in the literature. Electrical properties were estimated using published fibre membrane and myoplasm electrical values. Models of synaptic current pathways were designed taking into account the small size of the postsynaptic patch activated by a transmitter quantum. This analysis reveals a resistance "in series" between the ACh-sensitive interfold crest and the remainder of the muscle fibre. The calculated cytoplasmic resistance of an interfold is between 0.2 and 3 Mohms which is in the same range as the fibre DC input resistance. The calculated interfold resistance appears to be dependent on the fibre type, the age and the pathology. Functional roles of junctional folds and dendritic spines are discussed.

Acetylcholine↗

PubMed1988

Classes of unitary evoked responses at the vertebrate neuromuscular junction.

Unitary evoked quantal responses at the frog and mouse neuromuscular junctions were found to have preferred latencies. Focal recordings indicate reproducibility of both conduction time and synaptic delay of the clustered responses. Clusters of responses with similar time courses which often occurred together in series of 100 responses were detected using a microcomputer program. These results demonstrate that the probability of release is not uniform along the length of the terminal.

Action Potentials↗

PubMed1988

[The effects of heptaminol chlorhydrate on neuromuscular transmission].

6-Amino-2-methyl-2-heptanol chlorhydrate, heptaminol chlorhydrate, blocks the response to indirect stimulation of the mouse diaphragm in vitro. This effect is due to a dose-dependent pre- and post-synaptic block of neuromuscular transmission starting at 1 mM heptaminol (HEPT). The complete block of neuromuscular transmission occurs at 10 mM. At 2 mM, the decrease in quantal size is more significant in the presence of d-tubocurarine than when the extracellular calcium is lowered. At this concentration, heptaminol also prolongs the depolarization time of the motor end plate potential. Slightly higher concentrations of heptaminol produce a decrease in quantal content. This latter effect is associated with an increase in synaptic delay.

Amino Alcohols↗

PubMed1986

Subunits in quantal transmission at the mouse neuromuscular junction: tests of peak intervals in amplitude distributions.

The regular spacing of peaks throughout the amplitude distribution of miniature end-plate potentials, quantal evoked end-plate potentials and quantal currents was demonstrated using autocorrelations and power density spectra calculated from the number of events in the successive bins of the histograms built by Matteson et al. (1979), Kriebel & Florey (1983) and Erxleben & Kriebel (1984). At the same mouse neuromuscular junction, the calculated interpeak was constant for evoked and spontaneous quantal releases, throughout sequential sampling and after change of bin size. The presence of regular peak intervals supports the hypothesis that quantal potentials are composed of potential subunits the size of the smallest subminiature potential. Challenging the hypothesis of an acetylcholine quantum composed of acetylcholine subunits, a postsynaptic origin of the subunit is proposed on the basis of the spatial arrangement in rows of the ACh receptors. The ACh-saturating patch evoked by a quantum release (Land et al., 1980, 1981) activates 10-20 rows of receptors, which is roughly the number of subunits composing a quantal event. Therefore the position of the ACh patch or the continuous variations in its size might cause stepwise variations in the total number of ACh receptors activated by an ACh quantum.

Acetylcholine↗

PubMed1985

Quail neural crest cells transformed by Rous sarcoma virus can be established into differentiating permanent cell cultures.

Quail neural crest cells derived from the truncal neural primordium, infected in vitro by Rous sarcoma virus (RSV) in January 1978, were induced to multiply and have been established into permanent cultures. These cultures contain cells that differentiate into melanocytes, neuron-like cells and flat cells. About 50% of these different cell types are tetanus-toxin positive. Electrophysiological studies have shown that some cells can generate action potentials similar to those reported in quail neural crest primary cultures. Taken together these data show that the RSV-transformed quail neural crest permanent cultures are composed of stem cells which can differentiate into cell types specific for neural crest.

Animals↗

PubMed1981

[Characteristics of the unitary end-plate potential of the frog (author's transl)].

When the transmitter release is reduced in physiological solutions with low calcium and high magnesium content (70% of transmission failure), most of the end-plate potentials are evoked by the release of one quantum. Observing a short sequence of the unitary potentials (10-30), one can see that they distribute into a few patterns of similar amplitude, latency and time to peak. The amplitude and latency frequency distributions show successions of peaks frequently set at regular intervals. The average number of peaks is 11 for the amplitude distribution and 14.5 for the latency distributions. Lowering the temperature (20-10 degrees C) lengthens the interval between the latency peaks (Q10 = 2). These observations suggest the existence of a limited number of releasing sites set at regular intervals (10-20 micrometers) along the branches of the nerve terminal. This hypothesis is discussed with regard to the well-known interpretation of the amplitude sub-units of the unitary response and the sub-miniature potentials. The time-distribution of the unitary-evoked potentials of identical latency is frequently periodic (about 60 s at 4 HZ of frequency stimulation). Slightly different frequencies can be observed at different latency values. Through our interpretation of the sub-units, these facts mean that the few active zones follow a periodic process which can explain the periodic oscillation already shown for the total activity of the neuromuscular junction.

Action Potentials↗

PubMed1979

[Evolution in time of transmitter release at frog neuromuscular junction].

It has previously been shown that the unitary quantal end-plate potentials observed at synapses blocked by low Ca++ high Mg++ ringer, belong to distinct clusters according to their amplitude, time to peak and latency characteristics. These clusters correspond probably to distinct releasing units dispersed along the presynaptic terminal branches. The distribution versus time of the occurence of unitary potentials belonging to one latency cluster has been studied over long lasting evoked nerve activity (stimulation frequencies: 1 to 10 Hz). It was observed that transmitter release at one releasing site is statistically periodic with emitting periods separated by rest periods. At a given end-plate the period of emitting activity seems to be independent from one emitting site to another.

Animals↗

PubMed1979

[Existence of different populations of unitary evoked postsynaptic potentials at the frog neuromuscular junction].

The postsynaptic response to monoquantic evoked transmitter release (mean quantal content about 0.3) has been studied at temperatures from 10 to 23 degrees C. The delay between nerve stimulation (1 to 10 Hz) and the unitary postsynaptic potentials fluctuates by steps. The existence of preferential delay sites can always be detected (mean number 13.5 +/- 3.1). Identical delay unitary postsynaptic potentials often shows identical amplitude and identical time to peak. These results suggest that few emitting sites are preferentially activated along the nerve terminal at low level release during long lasting stimulation. The "single process" assumption used in statistical studies of transmitter release is probably oversimplified.

Animals↗

PubMed1977

[Periodic variations in motor endplate potentials].

The amplitudes of motor end-plate potentials in the Frog have been measured during repetitive stimulations of the motor nerve at frequencies between 0,5 and 30 Hz. A serial analysis showed that cyclic variations of these amplitudes were superimposed on the random fluctuation of epp due to the quantal nature of transmitter release. The period of these cyclic variations varied between 3 and 10 stimulations. This period was not significantly related to the frequency of the stimulation. The frequency spectrum analysis of the epp amplitudes showed similar values for the period of the cyclic variation of epp. The same kind of analysis applied to a binomial series of random numbers did not significantly present periodic fluctuations. The same analysis was applied to mean amplitudes of groups of 5 to 120 successive epps at the same end-plate. Periodic fluctuations were identified with periods of about 10 to 180 sec. It is suggested that periodic failures of nerve spike propagation in nerve terminals at sites of low safety factor, as at branchings, are responsible for the observed cyclic variation of epp.

Animals↗

PubMedDate not supplied

Transmitter release: prepackaging and random mechanism or dynamic and deterministic process.

Stepwise variations in end-plate potential amplitudes that are also multiples of spontaneous miniature end-plate potentials (MEPPs) demonstrate a quantal nature of evoked transmitter release at the vertebrate neuromuscular junction. Both the number of quanta which form relatively small end-plate potentials (EPPs) and the time intervals between MEPPs were found to fit Poisson statistics. These observations suggested that the release process randomly liberates uniform quantities of transmitter. Initial studies showed that quantal size remained stable after seemingly high rates of release which was interpreted to indicate that a large store of equally sized, equally available, and independently releasable quanta are present in the nerve terminals. The observation of numerous presynaptic vesicles that contain transmitter provided a morphological basis for prepacked transmitter (i.e., quanta). However, physiological studies over the last 15 years have yielded data that are difficult to incorporate into the quantum-vesicle hypothesis. With normal conditions and during most treatments which increase the rate of release, two classes of MEPPs have been found and both show a substructure. The bell-MEPP class was characterized by Fatt and Katz and the smaller skew-MEPP class has been studied by Kriebel. The ratio of the two classes and substructure compositions of both classes are variable. Short series of MEPPs and unitary EPPs (U-EPPs) show preferred amplitudes and longer series of MEPPs and U-EPPs show stepwise variations in amplitude. Slow-MEPPs and giant MEPPs belong to the skew class and represent nearly synchronous bursts of smaller MEPPs. Transmitter packet formation, preferred amplitudes, stepwise variations in amplitudes, random-like distributions and organized bursts can be simulated by a simple deterministic system, the drop formation process, that is known for its periodic and chaotic behaviors which are determined by the single parameter of flow rate. MEPP intervals, sizes and classes, are also dependent on rates of release which demonstrate that the release process(es) is highly organized and sensitive to different conditions. We demonstrate that the processes of drop formation and release of a packet of transmitter have similar properties and that deterministic characteristics describe MEPP and U-EPP time dependencies and amplitude substructures. The data and model presented here suggest that packet size of acetylcholine may be determined at the moment of release.

Animals↗