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Biomedical subjects

J Vojtassák

Publications and source records attributed to J Vojtassák.

At least 19 recordsLinked to original sources

[Unusual infection complication of total hip arthroplasty].

Authors present the case history of a 66-year old patient after repeated reimplantations of the THA with a deep infect caused by a rare aetiological agent (Serratia marcescens) associated with a pyogenic sinus. They describe the disease history, therapeutic procedure, complications associated with the surgery as well as postoperative course after the reimplantation of a customized total hip replacement. In the conclusion they state that in case of an infected total hip arthroplasty the treatment is focused on the salvage of the infection process and preservation of the function of the affected limb. Of essential importance is surgical revision with a radical removal of necrotic tissues and hardware in combination with an intensive parenteral antibiotic administration.

Aged↗

[Incorporation of bone allografts with the use of Octopus revision system in total hip arthroplasty].

PURPOSE OF THE STUDY: The study describes the revision arthroplasty of hip joints with grade III acetabular defects, as rated according to Paprosky, employing morsellized bone allografts and the Octopus revision acetabular system. MATERIAL: In the period from 1998 to 2002, a total of 8297 operations were performed in our Department; of these 1527 (18.4%) were total hip replacements. In this group, 267 (15.5%) revision arthroplasties were carried out, with the the Octopus revision system being used in 32 patients (age range, 30 to 79 years; average age, 60.3 years). The average period between the initial surgery and reimplantation was 6.5 (range, 2-14) years. METHODS: Acetabular defects were filled with morsellized cancellous bone allografts (one to three femoral heads per patient) obtained from the tissue bank. In nine patients, allografts were combined with hydroxypatite (Biovan). Osteointegration of the bone allografts and, if it occurred, loosening of the implant were checked by X-ray examination. RESULTS: The patients were examined at intervals of two months. The average Harris hip score was 76 points at 1 to 3 years. None of the patients had deep post-operative wound infection; in one patient, the distal hook of the Octopus system broke off. The complete incorporation of allografts was seen on X-ray images in 28 patients; at follow-up, a radiolucent zone between the acetabular bed and the allograft was still visible in four patients. No implant loosening was recorded. DISCUSSION: The Octopus system has an advantage of using morsellized allografts instead of solid ones that are associated with poorer incorporation and a higher risk of loosening. The remodeling of an allograft below the ring leads to the production of bone tissue, which is useful if revision arthroplasty were to be performed. CONCLUSION: Based on their experience, the authors regard the Octopus revision system with the use of morsellized bone allografts from the tissue bank as an effective surgical procedure for the treatment of Paprosky grade III acetabular defects. The allografts below the ring are fully incorporated and remodeled; as a result the initial defect is gradual filling with the patient's own tissue, which has very good clinical outcomes.

Adult↗

[Use of RT-PCR in the detection of circulating micrometastases].

During assessment of the progression of several types of carcinomas, such as cancer of the breast, lungs, prostate or urinary bladder world-wide the presence of circulating cells (micrometastases) in the circulation is followed up. These methods are gradually introduced also in Slovakia. First we tried in the Institute of Medical Biology and Genetics Medical Faculty Comenius University in collaboration with the Urological Clinic of Dérers the Faculty Hospital with policlinic to apply this method in carcinoma of the prostate (CaP). We detected the presence of epithelial prostate cells in the peripheral blood stream of patients with advanced prostate cancer where before secondaries were not detected.

Humans↗

[DNA analysis in gestational trophoblastic disease].

OBJECTIVE: DNA analysis of different forms of gestational trophoblastic disease. DESIGN: Retrospective clinical study. SETTING: Slovak Center of Trophoblastic Disease, Bratislava, Slovak Republic. METHODS: In the period of September 1993 to April 2003, eighty-nine cases of gestational trophoblastic disease were analysed. There were 22 cases of partial hydatidiform moles, 58 cases of complete hydatidiform mole, 5 cases of invasive mole and 4 cases of gestational choriocarcinomas. Southern hybridization and polymerase chain reaction were used for DNA analysis. RESULTS: From 22 analyzed cases of partial hydatidiform moles 19 (86.4%) were triploid and 3 (13.6%) diploid ones. There were 58 cases of complete hydatidiform mole and out of them 29 (50%) were homozygous, 28 (48.3%) heterozygous, and in one case (1.7%) both paternal and maternal genome was detected. In 8 cases of heterozygous and in one case of homozygous complete hydatidiform mole occurred a malignant transformation to gestational choriocarcinoma. CONCLUSIONS: Molecular analysis can determine the nuclear DNA origin of complete hydatidiform mole and allow us to define the patients with higher risk of malignant transformation usually to gestational choriocarcinoma.

DNA, Neoplasm↗

In vitro cytotoxicity testing of coladerm membrane.

At present, biodegradable and biocompatible membranes based on collagen and glycosaminoglycans play an important role in substitutive medicine. Modern biomaterials use a chemically modified collagen-based matrix for implants with programmable biodegradability as a substitute of buccal mucosa, skin, cartilage, etc. Besides the requirements for biocompatibility and biodegradability, the membranes must be also non-toxic. Therefore, cytotoxicity testing of these materials in vitro is an integral part of introducing newly developed types of membranes into clinical practice. As a biological model for the tested COLADERM membrane, cell cultures from human embryonic fibroblasts (B-HEF-2) were used for both cytotoxicity testing as well as in tests to assess the ability of cells to proliferate on this membrane. Along with the ability of cells to grow on the surface and inside the membrane, immunohistochemical examination and scanning electron microscopy (SEM) were performed as well. The obtained results have shown that the COLADERM membrane is non-toxic with suitable structural and biological properties for clinical application as a substitute of buccal mucosa following surgical ablation of malignant tissues from the oral cavity.

Journal Article↗

[Fertility after chemotherapy of gestational trophoblastic disease].

OBJECTIVE: To evaluate subsequent pregnancy experience in patients following chemotherapy for malignant form of gestational trophoblastic disease. DESIGN: Retrospective clinical study. SETTING: Slovak Center of Trophoblastic Disease, Bratislava, Slovak Republic. METHODS: There were evaluated subsequent pregnancy experiences in 38 patients after chemotherapy for malignant form of gestational trophoblastic disease registered in Slovak center of gestational trophoblastic disease. Histological and cytogenetical analysis of all placentas after deliveries and material from curettage specimens after miscarriages, abortions and ectopic pregnancies were performed. RESULTS: 11 women conceived following successful chemotherapy of gestational trophoblastic disease became pregnant a total 19 times. Out of them there were 9 full-term deliveries, 2 spontaneous abortion, 1 ended in ectopic pregnancy and 7 pregnancies were terminated in therapeutic abortion. Cytogenetical analysis was successful in 7 to 10 reproductive losses with normal karyotype in all analysed cases. CONCLUSION: Patients after successful chemotherapy of gestational trophoblastic disease have a normal reproductive outcome.

Antineoplastic Agents↗

Developmental defects and chromosomal aberrations in spontaneous abortions and stillbirths.

The authors analysed 1488 cases of spontaneous abortions and stillbirths in Bratislava. They focused on the course of human embryogenesis and the chromosomal constitution. A high mean frequency rate of both developmental defects (14.4%) and chromosomal aberrations (33.6%) was revealed and both were found to be in close relation with the length of gestation. The most severe developmental defects occurred mostly in early stages of human embryogenesis, i.e. in the 1st trimester of gestation.

Abortion, Spontaneous↗

Mola invasiva--special form of GTD.

Invasive hydatidiform mole is a relative rare form of gestational trophoblastic disease (GTD). Most of hydatidiform moles remit after evacuation but some of them have the tendency to invade the myometrium. In some rare cases the trophoblastic tissue can be found in other tissues like lungs, vulva, vagina or broad ligament. The aim of the study was to demonstrate some of clinical, immunohistochemical and DNA analysis findings of a patient with a previous diagnosis of a complete hydatidiform mole.

Base Sequence↗

[Personal experience with diagnosis of complete hydatidiform mole based on DNA].

In our study we analyzed 13 cases of histologically defined complete hydatidiform mole (CHM), by using cytogenetic and molecular genetic methods. There were seven homozygous and six heterozygous CHM. In one case of heterozygous CHM we found a biparental contribution to genomic DNA. This is evidence of a more heterogeneous etiopathogenesis of hydatidiform mole.

DNA, Neoplasm↗

[Diagnosis of partial hydatidiform mole using molecular genetics].

The aim of our study was to confirm the diagnosis of partial hydatidiform mole (PMH) and to determine mechanism of PMH development by chromosomal DNA analysis. In our study we analysed 8 cases of morphologically and histologically confirmed PMH. Their karyotype was determined by using methods of direct, 24-hour and a long-term tissue culture. The restriction fragment length polymorphisms (RFLP) method was used for DNA analysis of PMH chorionic villi and from progenitor lymphocytes in peripheral blood. All cases were triploid. DNA analysis revealed in six cases one maternal and two paternal RFLP bands, and in two cases two maternal and one paternal RFLP bands.

DNA, Neoplasm↗

[Advances in DPX densitometry].

The author provides information on modern densitometric examinations and their development in the past three decades. He analyses in brief the possibilities of densitometry on the basis of the double x-ray absorptiometry on the basis of his own experience and data gained from literature. The current standard software equipment includes the measurement of the lumbal PA projection, lumbal lateral, coxae PA and entire body PA. The apparatus detects the quantity of minerals in bones in form of BMC (bone mineral content) and BMD (bone mineral density) values, and compares the measured values of density with the average values in the healthy population. It automatically evaluates statistical values of the Z and T score. The bone density can be measured with the accuracy of 3-5%, the error of reproducibility is 1-2%. The apparatus represents an ideal equipment for the measurement of bone density under normal anatomic conditions of the skeleton and in early stages of the disease which is manifestant by changes in bone tissue. As soon as the condition includes deformations of the skeleton and osteoproductive changes, especially osteochondrosis, spondylosis and the presence of osteophytes, the result becomes inaccurate. The study drafts the trend in densitometry in frame of therapeutic, preventive and research activities in orthopaedics. (Fig. 4, Ref. 16.)

Absorptiometry, Photon↗

[Trophoblastic disease. I. Use of immunohistochemistry in the diagnosis of complete hydatidiform moles].

Immunohistochemical study of 15 complete moles detected expression of human chorion-gonadotropin (hCG), human placental lactogen (hPL) and placental alkaline phosphatase (PLAP). Syncytiotrophoblast showed the most conspicuous expression of hCG, hPL positivity was mild, PLAP positivity poor. Intermediate trophoblast had stronger hPL positivity There was not any positivity in cytotrophoblastic cells.

Alkaline Phosphatase↗

[Trophoblastic disease. II. Immunohistochemical and cytogenetic parameters of partial hydatidiform moles].

A group of 30 cases of partial hydatid mole were studied by cytogenetics and immunohistochemistry. Trophoblastic cells were always triploid. Syncytiotrophoblastic cells and intermediate trophoblast showed the strongest expression of PLAP, hPL positivity was mild. Expression of hCG was only focal. There was not found any detectable immunopositivity in trophoblastic cells.

Alkaline Phosphatase↗

Interferon alpha2b is the predominant subvariant detected in human genomic DNAs.

Chromosomal DNAs isolated from eight individuals from the Slovak population and from lymphoblastoid Namalwa cells were analyzed for the presence of genes coding for three subvariants of human interferon-alpha 2 (IFN-alpha 2), namely a, b, and c. The respective genes are regarded allelic, because they differ in the coding nucleotide sequence only at the position 137 (a:A, b/c:G) and/or at the position 171 (a/b:A, c:G). IFN-alpha 2 sequences in genomes were selectively amplified using polymerase chain reaction (PCR). Resulting "consensus" PCR-product (the total mixture of PCR-derived clones) was sequenced and the subvariant-specific nucleotides at position 137 and 171 were determined. In one placental genomic DNA and in a mixture of genomic DNAs from leukocytes of seven donors only nucleotides specific for subvariant IFN-alpha 2b could be detected. This suggests that the placental DNA contained only genes coding for IFN-alpha 2b and these alleles were at least prevailing in donor's genomes. On the other hand, the majority of genomic alpha 2-sequences in Namalwa cells (from which IFN-alpha 2c was originally derived), seems to be corresponding to subvariant IFN-alpha 2c.

Alleles↗

[Intra-articular and periarticular punctures and injection therapy in clinical practice].

The author presents his own clinical experience in intra and periarticular injection therapy. He analyses 54 421 patients examined within the period of 10 years. The list of frequency of affections, due to which the patients were examined, is given in the following order: spine 27.4%, coxae 18.6%, knees 18.2%, ankles and feet 14.5%. The intraarticular and periarticular injections were most often applied into the knee 38%, arm 22%, and coxae 17%. Since the evaluation is arduous, the year 1991 was chosen to be analyzed, as the total of examinations in this year was 2774. Intraarticular and periarticular injections represent here a proportion of 15%. During this year 416 intraarticular and periarticular injections were performed, which would represent app. 4160 therapeutic interventions if recalculated per ten years. During this period cortisonoid arthropathy occurred twice (0.12%) and infectious arthritis once (0.024%). The best clinical results were accomplished following application of corticoids which were administered in 222 cases (53.4%). Yet in these cases local and general side-effects occurred. (Tab. 4, Ref. 11.)

Female↗

[Intra-articular and peri-articular injection therapy].

The author in the article in the form of an assembled report deals with the problem of intraarticular and periarticular injection therapy. The author divides the matter in two parts, the diagnostics and therapy. In general, there are four possibilities of influencing the joint by means of an injection. First, the evacuation of intraarticular contents, second, the introducing of pharmacy into the joint, third, the mechanical irritation of certain connective tissue structures of the joint which ought to evoke the formation of a scar, and fourth, the mechanical stabilization of the structure inflicted by lesion. Owing to the removal of pain, the negative effects of neuromuscular reflexes due to algesic stimuli, are eliminated. There are two basic types of affections subjectable to injection intraarticular treatment, namely the diseases coinciding with the damage of articular biomechanics, particularly disturbances of both, the sliding in arthrosis and articular stability. In such cases the application of chondroprotectives, eventually formation of a scar by irritation of the appropriate connective tissue structures, decreasing of instability and removal of contractures are theoretically justified. Evacuation punction is indicated in post-traumatic states, inflammatory states indicate diagnostic punction, and aseptic inflammation requires infiltration of pharmacy with antiphlogistic effect. Injection intrarticular intervention represents a justified diagnostic procedure and an effective therapeutical administration. It is necessary to become familiar with the topographic anatomy of joints, just as with the technique of application, contraindications, and determination of possible limitations of intraarticular treatment and surgical reconstructive intervention into the joint or extremity. The most serious complications of intraarticular injection administration are represented by iatrogenic infections and cortisone-like arthropathies.(ABSTRACT TRUNCATED AT 250 WORDS)

Humans↗

[Dual x-ray absorptiometry in the diagnosis of osteoporosis].

The author presents a review providing information on contemporary modern radiological possibilities as regards the diagnosis of osteoporosis at the First Orthopaedic Clinic in Bratislava. At present this department possesses a densitometer LUNAR DPX-L. This apparatus assesses the bone density on the basis of double X-ray absorption. The software available at present makes it possible to assess the bone density of the lumbar spine in the anterio-posterior and lateral projection, as well as the proximal femur and whole body. The mean time required for assessment is 4 mins. 24 secs. to 16 mins. 30 secs. The mean radiation load of the patient is 1.5 mrem, the maximal load 3.5 mrem. The apparatus compares the assessed density values with mean values of the healthy population, it evaluates automatically statistical values of the Z and T score. These results are presented either as a coloured graph or as values of bone mineral density and bone mineral content. The author explains in more detail the principle of modern densitometry and its development during the past three decades.

Absorptiometry, Photon↗