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Biomedical subjects

J Vuddamalay

Publications and source records attributed to J Vuddamalay.

2 recordsLinked to original sources

Plastic specula: can we ease the passage?

BACKGROUND: For many years, genitourinary physicians have taught that specula should be used without lubrication other than water, as it was assumed that gel would interfere with the processing of samples, but there seems little evidence to support this. Many clinics are now using plastic specula to avoid reusable instruments, and one of the commonest problems with such specula is increased friction. METHODS: We looked at the effect of Aquagel on the culture of different dilutions of Neisseria gonorrhoeae on three standard laboratory media. The effect of Aquagel on the chlamydial strand displacement assay (SDA) test was also assessed by mixing different amounts of Aquagel with the positive and negative control and processing in the usual way. RESULTS: There was found to be no inhibition of culture following emulsification of N gonorrhoeae in Aquagel at any concentration. All the results for the chlamydial SDA test were satisfactory following mixing with Aquagel. CONCLUSION: We think that the clinician should now be more confident that if a difficult examination requires the use of a lubricant, the test results will not be compromised.

Chlamydia Infections↗

Role of type specific herpes simplex virus serology in the diagnosis and management of genital herpes.

OBJECTIVES: To investigate the indications for the use of a type specific antibody test for herpes simplex virus in a department of genitourinary medicine in the United Kingdom. METHOD: Retrospective analysis of case records of 127 patients who accepted the test during a 20 month period. RESULTS/CONCLUSION: The test contributed to patient management in 79% of patients with recurrent genital ulceration of unknown cause. It was also useful for counselling a number of patients with initial episodes of disease and the asymptomatic partners of some patients when the partners were shown to possess antibodies specific to herpes simplex virus type 2. When evaluating sexual partners, the test was difficult to interpret if an isolate from the index case had not been typed. Access to viral typing may therefore be a greater priority than serological testing. As adverse psychological sequelae may follow the identification of an asymptomatic chronic infection, guidelines for the use of a type specific serological test are proposed.

Antibodies, Viral↗