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Biomedical subjects

J W Gunnink

Publications and source records attributed to J W Gunnink.

7 recordsLinked to original sources

Retained placenta and leucocytic activity.

In a study of 262 cows, the relationship between the activity of peripheral leucocytes and the expulsion of the placenta was investigated, bearing in mind that the post- partal placenta may be considered as a foreign tissue. The activity of the leucocytes against the cotyledon was examined by determining the presence of chemotaxis in a Boyden Chamber, and by estimating the number of leucocytes in the cotyledon at the time of parturition. It was shown that in the absence of leucocytic activity, retained placenta occurred in 100 per cent of cases. When chemotaxis is used as the sole criterion, positive chemotaxis resulted in an incidence of 2.6 per cent retained placenta and negative chemotaxis in 35.6 per cent.

Animals↗

Pre-partum leucocytic activity and retained placenta.

An investigation concerning the association of the leucocytecotyledon reaction and the expulsion of the placenta is described. The in vitro reaction does not appear to be individual-specific. It is thus possible to determine the activity of the leucocytes against a cotyledon before parturition. The leucocytes of cows which will develop a retained placenta have lowered activity against the chemotactic stimulus of a cotyledon; this reduced activity of the leucocytes can be observed some days before parturition.

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Post-partum leucocytic activity and its relationship to caesarian section and retained placenta.

The activity of the leucocytes of 30 cows during 10 days after parturition is described. A comparison is made between the activity of leucocytes of 10 cows after normal parturition, 10 cows after caesarian section and 10 cows with retained placenta. When compared with normal parturition, caesarian section does not have a negative influence on the activity of the leucocytes during the puerperal period, during which time however the activity of leucocytes of cows with retained placenta is strongly decreased.

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Influence of dilution on the chemotactic properties of cotyledon suspensions.

In this study the influence of dilution of cotyledon suspensions on the chemotactic stimulus was investigated. When the concentration of the chemotactic stimulus is reduced, the activity of the leucocytes is reduced as well. Dilutions of retained placenta cotyledon suspensions showed an increase in leucocyte activity. This may indicate the existence of a leucocyte inhibition factor in the retained placenta cotyledon.

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[Diagnosis and treatment of postpartum endometritis in cattle].

The bovine genital tracts of 120 animals in which parturition had run a highly abnormal course and those of thirty which had calved normally and showed retained placentas were studied by vaginoscopic examination and bacteriological examination on the fifteenth, seventeenth and thirtieth day after parturition. The animals were classified at random into four groups. Three groups underwent intra-uterine treatment (with antibiotics, lotagen and polyvinylpyrrolidone iodine respectively) on the fifteenth day after parturition and one group remained untreated. The methods of treatment used did not result in more rapid healing of the uterus, which was verified by the results of vaginoscopic examinations as well as by those of bacteriological examination. An outstanding feature consisted in the fact that the uterus has an effective self-cleansing mechanism. Bacteriological examination was positive in 132 out of 150 animals on the fifteenth day after parturition. Only seventy-five animals were positive on the thirtieth day after parturition. The number of animals positive for C. pyogenes dropped from eighty-four (day 15) to forty-three (day 30). There was a marked correlation between the results of vaginoscopic examination and those of bacteriological examination of the uterus. When the discharge was mucopurulent or purulent thirty days after parturition, bacteriological examination was positive in 64 and 74 per cent of the cases respectively. C. pyogenes could be isolated in 74 and 93 per cent of the cases respectively and usually was present in the form of pure cultures. It is concluded that there is no point in carrying out a single intra-uterine treatment about the fifteenth day after parturition in cattle affected with endometritis.

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[Vaginitis in the bitch (author's transl)].

During a period of two and a half years (2 1/2), sixty-two patients wre submitted to the Department of Veterinary Obstetrics and Gynaecology, where a diagnosis of vaginitis was established. These patients often were young large dogs. Most of the females showed a vaginal discharge, although some bitches were only very attrative to males. There were no complaints concerning the general physical condition of the patients. On clinical examination, the vulva was found to be swollen. Exudation was also frequently observed, externally (vaginoscopy). Ninety per cent of the patients showed normal haemograms. On bacteriological examination, streptococci, E. coli and pasteurellae were often isolated. As a rule the bitches were treated locally (deeply intravaginal), the choice of the drug depending on the results of bacteriological examination. Another bacteriological examination was made after treatment. An inquiry was made into the results of treatment in these sixty-two dogs, wherever possible. On the other hand, the prognosis in those bitches in which bacteriological examination is negative at the time of discharge from the department is likely to be better than it is in those in which bacteriological examination is still positive at that time. The extent to which vaginitis may influence fertility is not known at the time of writing. The differential diagnosis of vaginitis and endometritis may present difficulties.

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