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Biomedical subjects

J W Hood

Publications and source records attributed to J W Hood.

10 recordsLinked to original sources

Comparative susceptibility of embryonated hen eggs and pig lung alveolar macrophages to chlamydial infection.

Pig lung alveolar macrophages provided a suitable cell culture system for primary isolation of ovine chlamydia and sustained their growth through several passages. In centrifuged preparations, titres of established strains compared favourably with those obtained in fertile eggs, and in parallel titrations on primary isolation, evidence of chlamydial infection was detectable in macrophages several days earlier than in eggs.

Animals↗

Growth of chlamydia in pig lung alveolar macrophages; preparation of macrophages and demonstration of growth.

The harvesting, storing and utilisation in tissue culture of lung alveolar macrophages from 2-3 weeks old piglets were investigated. An average yield of 4 X 10(8) cells was obtained, which in culture usually remained healthy for at least 8 days. In liquid nitrogen at -173 degrees C about 50% of the cells were still viable after 3 months. Pig cells sustained the growth of ovine Chlamydia psittaci and a rising titre was demonstrated over 4 passages.

Animals↗

Application of the new Australian standard for clinical lighting.

A new Australian Standard "AS 1765-1975 Artificial Lighting for Clinical Observation" has recently been published. This specifies the quality of light necessary to accurately assess a patient's colour. The physical principles and terminology used in modern lighting are discussed, and the experimental work on which the new Standard is based is outlined. The only readily available fluorescent tubes which conform to the Standard are the imported Philips "TL40W/37" and the more efficient locally made "White Deluxe" tubes.

Australia↗

A new blood administration set.

A new blood administration set is described. It incorporates an in-line hand pump and a heat exchange coil and has a number of advantages over separate heating coils. The blood warming efficiency of the unit has been measured.

Blood Transfusion↗