Biomedical subjects

J W Hutter

Publications and source records attributed to J W Hutter.

24 records · Page 2Linked to original sources

PubMed1996

Production of interleukin-1 by polymorphonuclear leukocytes resident in periradicular tissue.

Twenty-one patients undergoing endodontic surgery were identified. Periradicular tissue samples were recovered, and those showing significant numbers of polymorphonuclear leukocyte (PMN) infiltration were prepared for immunoperoxidase identification of interleukin (IL)-1 alpha and IL-1 beta-producing cells using specific polyclonal antibodies. In selected tissue specimens, 90% or more of the PMN's were found to stain positively for IL-1 alpha and IL-1 beta. In addition, significant numbers of plasma cells and tissue histiocytes stained positively for these IL's. Cell suspensions from selected periapical granuloma specimens, as well as from purified peripheral blood PMN's and peripheral blood mononuclear cells, were also subjected to IL-1 quantitation using a commercial ELISA procedure. Such cell suspensions were found to produce significant levels of IL and could be stimulated to produce increased levels after coculture with lipopolysaccharide. These results suggest that PMN's in inflammatory periradicular tissues may be a significant source of IL-1, and their possible roles in the establishment and resolution of periradicular lesions need to be re-evaluated.

Animals↗

PubMed1996

An in vitro evaluation of microleakage of a new root canal sealer.

An in vitro dye leakage study was performed to compare the apical microleakage of Ketac-Endo root canal sealer with that of Roth's 801E and AH26 sealers. Sixty-four single-rooted human teeth were instrumented and randomly divided into four groups. Three groups were obturated with laterally condensed gutta-percha and either Roth's 801E, AH26, or Ketac-Endo as the sealer. A fourth group was obturated using a single master cone and the Ketac-Endo sealer. The teeth were suspended in 1% methylene blue dye for 6 days. The teeth were then longitudinally sectioned and evaluated for linear apical dye penetration. In general, Ketac-Endo root canal sealer showed greater dye penetration than Roth's 801E and AH26. There was no statistical difference in leakage between the laterally condensed Ketac-Endo group and the single-cone-obturated Ketac-Endo group.

Analysis of Variance↗

PubMed1996

The effect of acute normovolemic hemodilution (ANH) on myocardial contractility in anesthetized dogs.

The influence of severe acute normovolemic hemodilution (ANH) on myocardial contractility (MC) was investigated in 14 splenectomized, anesthetized dogs. MC was assessed by the maximum rate of left ventricular pressure increase (LVdp/dt(max)), end-systolic elastance (Ees), and preload recruitable stroke work (PRSW) (conductance catheter, left ventricular pressure-volume relationship). Measurements of myocardial perfusion and oxygenation (radioactive microsphere technique) assured comparability of the model to previously performed studies. Global and regional myocardial blood flow increased significantly upon hemodilution with preference to midmyocardium and subendocardium. This resulted in preservation of both myocardial oxygen delivery and consumption after ANH. Myocardial oxygen extraction as well as coronary venous Po2 were unaffected by ANH, while coronary venous lactate concentration decreased, indicating that myocardial oxygen need was met. LVdp/dt(max) decreased significantly after hemodilution (2278 +/- 577 vs 1884 +/- 381 mm Hg/s, P < 0.01), whereas Ees and PRSW increased significantly (1.76 +/- 0.54 vs 2.15 +/- 0.75 mm Hg/mL, P < 0.05, for Ees and 33 +/- 14 vs 45 +/- 14 mm Hg.mL, P < 0.05, for PRSW). While the decrease of LVdp/dt(max) most likely reflects ANH-induced changes of ventricular pre- and afterload, the increase of Ees and PRSW indicates a true increase of myocardial contractility during ANH in anesthetized dogs.

Anesthesia↗

PubMed1995

Leakage of amalgam and Super-EBA root-end fillings using two preparation techniques and surgical microscopy.

An in vitro dye leakage study was conducted to test the root-end sealing ability of amalgam with varnish or Super-EBA using two root-end preparation techniques and surgical microscopy. Sixty-four single-rooted teeth were instrumented and obturated with gutta-percha. Teeth were randomly placed into four groups. Two groups received perpendicular root-end resections, 3-mm-deep ultrasonic root-end preparations, and either amalgam with varnish or Super-EBA root-end fillings. The other two groups received beveled root-end resections, 3-mm-deep micro-handpiece preparations, and either amalgam with varnish or Super-EBA. All root-end procedures were performed at x5 to x8 magnification. Micro-leakage was assessed at 4 months using methylene blue dye and a passive hydrostatic pressure technique. Statistical analysis showed that, regardless of technique, Super-EBA leaked significantly less than amalgam with varnish. There was no significant difference between the two root-end resection and preparation techniques.

Chi-Square Distribution↗

PubMed1995

Central odontogenic fibroma mimicking a lesion of endodontic origin.

The central odontogenic fibroma is a rarely reported lesion that can be associated with vital teeth. Although it is uncommon and unlikely to recur after enucleation, the central odontogenic fibroma should be considered in a differential diagnosis of radiolucencies associated with vital teeth. This case report presents the radiographic and clinical signs of a central odontogenic fibroma occurring in the maxillary anterior region.

Adult↗

PubMed1994

Cast prosthesis removal using ultrasonics and a thermoplastic resin adhesive.

Nonsurgical endodontic procedures are routinely performed on teeth with existing cast restorations. The internal anatomy of the tooth and location of the root canals may be obscured by a crown or fixed partial denture. Tooth morphology and canal location may, therefore, be better visualized without the cast restoration in place. This article describes a conservative technique to remove an existing cast restoration to facilitate nonsurgical root canal treatment.

Crowns↗