Biomedical subjects

J W Kuan

Publications and source records attributed to J W Kuan.

4 recordsLinked to original sources

PubMed1977

An immobilized hexokinase enzyme stirrer for a simple and economical assay of plasma glucose.

We have adapted the hexokinase glucose procedure to an immobilized enzyme stirrer for the determination of glucose concentrations in human blood plasma. The procedure is a fluorometric rate method measuring the formation of NADPH catalyzed by immobilized glucose-6-phosphate dehydrogenase and hexokinase held within a tiny stirrer. The enzyme stirrer is stable for at least two months and can be used over eight-hundred assays without any loss of activity.

Blood Glucose↗

PubMed1977

Determination of plasma glucose with use of a stirrer containing immobilized glucose dehydrogenase.

A stirrer containing immobilized glucose dehydrogenase has been successfully used for determining glucose in plasma. The device is usable for at least two months and for about 500 assays. The reaction was measured kinetically and linearity was observed to 4 g of glucose per liter. Tested with aqueous glucose and with deproteinized plasma, within-day and day-to-day precision were good. Interference and method-comparison (hexokinase method) were examined. The performance of this system makes the technique useful and attractive for routine use in small-volume clinical laboratories.

Blood Glucose↗

PubMed1976

Measurement of glucose in plasma, with use of immobilized glucose oxidase and peroxidase.

We describe a novel fluorometric technique for simple, rapid, and economical assay of glucose by use of immobilized glucose oxidase and peroxidase. A cylindrical magnetic stirrer was specially designed to hold the immobilized enzymes firmly and to allow the reaction minzymic transformation quickly. Blood plasma can be assayed directly with no pretreatment. Ascrobic acid, uric acid, and creatinine in concenctrations of 0.25, 0.25, and 0.2 g/liter, respectively, did not interfere. The linearity of the assay was extended to 4.0 g of glucose concentrations. Results by our technique and by the otoluidine or hexokinase methods agreed well. The immobilized enzymes are stable for several months and can be used for several hundred highly accurate and reproducible assays.

Aspergillus niger↗

PubMed1975

Semi-solid surface fluorometric analysis of glucose.

We describe an enzymatic fluorometric method for determining glucose concentrations in blood samples by analysis on a semi-solid surface (silicone-rubber pads). The method is an adaptation of existing spectrophotometric procedures based on the sequential coupling reactions with glucose-6-phosphate dehydrogenase and NADP+. The rate of appearance of NADPH fluorescence at 460 nm (excitation wavelength, 340 nm) is monitored and related to the glucose concentration in plasma or serum. The calibration curve is linear to 2.50 g/liter. The chief advantages of this method over the spectrophotometric procedure are low cost and rapid response. The results obtained correlate well with those of the o-toluidine method (correlation coefficient, 0.986).

Binding Sites↗