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Biomedical subjects

J W Li

Publications and source records attributed to J W Li.

31 records · Page 2Linked to original sources
PubMed1991

[Acrocallosal syndrome--an autopsy case report and literature review].

This is an autopsy report of a female neonate with acrocallosal syndrome, which shows developmental retardation, callosal dysgenesis and preaxial hemimelia of the upper limbs. Either dysgenesis or corpus callosum or congenital absence of radius is rare; their combination are even rarer and only eleven cases of acrocallosal syndrome have been reported in the English literature. Our case appears to be the first report in Taiwan. The family history and pregnant course were unremarkable except that her mother had taken Chinese herb medicine for common cold in the 4th pregnant month. Prenatal obstetric sonography showed "ventriculomegaly" at the 31st week. We reviewed the literature and discussed the incidence, embryology, pathogenesis, symptoms as well as etiology concerning this rare syndrome.

Abnormalities, Multiple↗
PubMed1991

[Diagnostic evaluation of the determination of antibodies to purified protein derivative in tuberculosis].

The results with ELISA method showed the levels of the antibody to PPD in exudates of TB group were significantly higher than those of non-TB group (P less than 0.001). When OD value greater than 0.2 and greater than 0.35 were chosen as the positive criteria, for diagnosing tuberculous meningitis and other tuberculous, the sensitivity would be 79.3% and 86.6%, respectively; the specificity would be 93.3% and 86.0%, respectively. Meanwhile tuberculosis could be suggested when the ratio of the antibody level in exudate to that in serum is greater than 1. The authors think that the detection of the antibody to PPD in exudates might be a supplemental diagnostic tool for tuberculosis.

Antibodies, Bacterial↗
PubMed1990

[A simple method for predicting equality of absorption and elimination rate constants].

A new method is proposed by which one can predict whether absorption rate constant (Ka) and elimination rate constant (Ke) are equal in the one-compartment open model with first-order processes. The method used only blood drug concentration-time data in the terminal segment of concentration versus time curve is described as follows. We defined Ai as Ai = 2/(tn + 1 + tn) + ln(cn/cn + 1)/(tn + 1 - tn) (i = 1,2,3,...) where n is the number of sampling sequence, cn and cn + 1, tn and tn + 1 are any two consecutive blood drug concentration (c)-time (t) data in the terminal segment of c-t curve, respectively. If Ai was equal or approximate, Ka and Ke might be equal or approach each other. In addition, both Ka and Ke were equivalent to the arithmetic mean of Ai. If Ai was reduced gradually while (tn + 1 + tn)/2 was increased, Ka might not be equal to Ke.

Absorption↗
PubMed1989

[Observation of immune function in 115 elderly patients with pulmonary tuberculosis].

This paper tested the indexes of the cellular immunity and the humoral immunity of initially treated 115 old patients with pulmonary tuberculosis, and the data were compared with these values of healthy elder and youth and middle aged patients with pulmonary tuberculosis. The results showed that the indexes of the cellular immunity elderly patients with pulmonary tuberculosis decreased (LTT Et-RFC Ea-RFC the discrepancy significantly, P less than 0.05), and the index of the humoral immunity increased (the varying of all the indexes P less than 0.01) compared with the health elders. All the immune indexes of the old pulmonary tuberculosis had no significant difference except for IgA compared with youth and middle aged patients.

Aged↗
PubMed1985

Suppression of defective-sporulation phenotypes by mutations in the major sigma factor gene (rpoD) of Bacillus subtilis.

Mutations (crsA47 and crsA4) in the major sigma factor gene (rpoD) of Bacillus subtilis RNA polymerase have been found to be powerful intergenic suppressors of spoOB, spoOE, spoOF, spoOK and spoIIG mutations. The crsA47 suppressor restores sporulation of spoOE, spoOF, spoOK and spoIIG mutants to levels near those of wild type bacteria and substantially improves the sporulation of a spoOB strain. The crsA mutations are shown to prevent the induction by aliphatic alcohols of SpoO phenocopies in wild type B. subtilis cells.

Bacillus subtilis↗
PubMed1982

Sun Simiao.

Explore the source record for details and available documents.

History, Medieval↗
PubMedDate not supplied

Expression of leukemia-inhibitory factor as an autocrinal growth factor in human medulloblastomas.

Medulloblastoma is the most frequent pediatric brain tumor, with the capacities of rapid proliferation and intracranial dissemination. However, the factor(s) regulating medulloblastoma growth has not yet been well characterized. Leukemia-inhibitory factor (LIF) and interleukin-6 (IL-6) play different roles in the formation/progression of various embryonic and pediatric tumors, but their biological effects on medulloblastoma cells are less well known. Therefore, in vivo and in vitro expression of LIF, IL-6 and their signal transducer genes encoding LIF receptor (LIFR), IL-6 receptor (IL-6R) and gp130 in human medulloblastoma cells were investigated by multiple cellular and molecular biology approaches. The results revealed that LIF expression could be found in 26 out of 28 tumors/cell line and over 90% of the samples expressed LIFR, IL-6R and gp130. In contrast, none of the samples showed IL-6 expression. An established medulloblastoma cell line, Med-3, was used to evaluate the potential effects of LIF and IL-6 on the proliferation of medulloblastoma cells. The growth of Med-3 cells was efficiently inhibited either by anti-LIF antibody or by antisense LIF oligonucleotide. Addition of exogenous human recombinant IL-6 could dramatically enhance Med-3 cell outgrowth. Our data thus for the first time demonstrated the important role of LIF as an autocrinal and IL-6 as a paracrinal growth factor in the proliferation of medulloblastoma cells.

Blotting, Southern↗
PubMedDate not supplied

All trans-retinoic acid suppresses in vitro growth and down-regulates LIF gene expression as well as telomerase activity of human medulloblastoma cells.

Leukemia inhibitory factor (LIF) is prevalently expressed in human medulloblastomas in vivo and in vitro. Blockage of LIF expression or biological function can inhibit in vitro growth and induce differentiation-like phenotypes of medulloblastoma cells (Med-3), suggesting a redifferentiation potential of Med-3 cells when intrinsic dedifferentiation factor(s) is suppressed or external differentiation element(s) is exerted. All-trans retinoic acid (RA) can induce differentiation in some types of cancers but its effect on medulloblastoma cells has not yet been described. To address the above issues, three dosages (5, 8 and 10 mumol/L) of RA were used to treat Med-3 cells and their effects on the target cells were evaluated by multiple approaches. It was found that, in addition to induction of morphological changes and growth inhibition, RA could repress LIF expression as well as the telomerase activity of Med-3 cells in time- and dose-related fashions. Our data thus suggest that the growth inhibitory effect of RA on Med-3 cells may be correlated with the LIF down-regulation. Reduction of telomerase activity by RA indicates that this enzymatic activity is a potential differentiation indicator of medulloblastoma cells.

Antineoplastic Agents↗
PubMedDate not supplied

Secretion of an anti-inflammatory, immunomodulatory factor by Schistosomulae of Schistosoma mansoni.

Penetration and migration of schistosomulae of Schistosoma mansoni through the skin of mice is associated with reduced inflammatory responses especially after a moderate infection. Previous studies to identify the mechanisms by which schistosomulae of S. mansoni suppress inflammatory responses in human skin showed that the excretory/secretory (ES) products of schistosomulae of s. mansoni contain activities that induce production of the antiinflammatory cytokine IL-1ra from human keratinocytes. In the present study we have characterized the ES products of the schistosomulae of S. mansoni to identify the IL-1ra inducing activity. We demonstrate that this anti-inflammatory activity is associated with a protein of molecular mass 16.8 kDa (Sm 16.8). Depletion studies confirm that Sm 16.8 is the major IL-1ra inducing activity in the ES products. A comparison of the proteins in the ES products of schistosomulae of S. mansoni with those of Trichobilharzia ocellata, a bird schistosome that induces an acute inflammation in human skin, shows that Sm 16.8 is absent in the ES products of T. ocellata. Interestingly, ES products of the schistosomulae of T. ocellata were potent inducers of IL-1 alpha from human keratinocytes, whereas ES products from schistosomulae of s. mansoni induce little or no IL-1 alpha secretion from keratinocytes. Functional studies show that Sm 16.8 suppresses antigen induced lymphoproliferative responses in vitro. Addition of Sm 16.8 to spleen or axillary lymph node cell cultures resulted in a significant reduction in antigen induced IL-2 secretion. These studies show that schistosomulae of S. mansoni elaborate an anti-inflammatory, immunomodulatory factor that may help the parasite to evade host immune responses in the skin. Given the capabilities of Sm 16.8 to induce IL-ira and suppress lymphoproliferation, this protein may also have a potential use as a therapeutic agent for inflammatory skin disorders.

Animals↗