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J W MacInnes

Publications and source records attributed to J W MacInnes.

At least 19 recordsLinked to original sources
PubMed1973

A quantitative analysis of the genetics of resting blood lactic acid levels in mice.

Resting blood lactate levels were measured in inbred mouse strains, their F(1), and several of their segregating generations to determine whether the level of lactic acid is influenced by genetic factors. The inbred strains in each of the two sets used differed significantly from one another for this character. Only one strain showed a significant sex difference. The data could not be fully analyzed because of the failure to fulfill Mather's first criterion for an adequate scale. Nonallelic interactions, in particular, additive x dominance and dominance x dominance, were found to influence the generation means. Genotype x environment interaction was detected and eliminated by log transformation. Negative heterosis was exhibited by all but one noninbred generation.-The data suggest that genes influencing the character are dispersed between the parental lines and that interactions are predominantly of the duplicate kind. A buffering system by which lactate levels are kept at a minimum is proposed.

Analysis of Variance↗
PubMed1967

Thermal depolarization of fluorescence from polytene chromosomes stained with acridine orange.

The degree of polarization of fluorescence from stretched Chironomus thummi polytene chromosomes, stained with low concentrations of acridine orange (AO), decreases with increasing temperature. The "half temperature" of this decrease (T((1/2)R)) is lower than the expected DNA thermal denaturation temperature (T(m)) by about 20 degrees C. T((1/2)R) is lowered as histone is removed from chromosomes. Balbiani ring regions of the fourth chromosome have T((1/2)R)'s much lower than other regions, and nearly as low as chromosomes which had been extensively pretreated with trypsin to remove histone and other proteins. Measurements of the thermal change in the rotational diffusion rate of AO in solution with DNA indicate that the temperature at which the DNA-AO bonding changes from a "rigid" to a "loose" mode varies with the GC percentage of the DNA, and in the same fashion as T(m), although 20 degrees C lower.

Acridines↗