Biomedical subjects

J W Rasburn

Publications and source records attributed to J W Rasburn.

2 recordsLinked to original sources

PubMed2001

Fluorogenic substrates for the detection of microbial nitroreductases.

AIMS: To synthesize and evaluate fluorogenic substrates for the detection of microbial nitroreductases. These substrates, all based on 7-nitrocoumarin, may be reduced to form fluorescent aminocoumarins. METHODS AND RESULTS: Thirty pathogenic microbial strains, including both bacteria and yeasts, were examined for nitroreductase activity in a whole-cell assay. All strains readily reduced each of the seven substrates to generate fluorescence, suggesting the widespread presence of nitroreductase activity in pathogenic bacteria. CONCLUSIONS, SIGNIFICANCE AND IMPACT OF THE STUDY: These novel substrates facilitate the direct detection of nitroreductase activity and have potential as sensitive indicators of microbial growth.

Bacteria↗

PubMed1986

Sensitive reagents for detection of indole production by bacteria.

A number of substituted 4-aminobenzaldehydes were compared with more commonly employed reagents for the detection of indole-producing organisms among the Enterobacteriaceae. Factors such as rate of colour intensity and stability were compared in tests with reference organisms and clinical isolates, and with appropriate concentrations of pure indolic standards. 2-Methoxy 4-dimethylaminobenzaldehyde and 4-N pyrrolidylbenzaldehyde produced immediate and intense red to purple colourations when used in the indole test both in liquid media and on multipoint inoculated plates. Both reagents were considered to be superior to the traditional Ehrlich's reagent and also to 4-dimethylaminocinnamaldehyde which is often used as an alternative.

Benzaldehydes↗