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Biomedical subjects

J W Rippon

Publications and source records attributed to J W Rippon.

At least 55 records · Page 3Linked to original sources
PubMed1971

Determination of fungal elastase.

The elastin-agar plate is useful for determining elastolytic activity. Determination of fungal elastase, however, requires partial purification of culture extracts and the orcein elastin or gravimetric method.

Acetone↗
PubMed1968

Monitored environment system to control cell growth, morphology, and metabolic rate in fungi by oxidation-reduction potentials.

A device for the adjustment, maintenance, and monitoring of a desired oxidation-reduction potential in a liquid environment is described. The filamentous species, Penicillium lilacinum and Trichophyton schoenleinii, and the dimorphic species, Histoplasma capsulatum, developed budding yeastlike cells when grown in a liquid medium with a low oxidation-reduction potential. When the organisms were inoculated into fresh medium, they returned to the filamentous condition as the oxidation-reduction potential was restored to a more positive (oxidizing) environment.

Candida↗
PubMed1968

Extracellular collagenase from Trichophyton schoenleinii.

The dermatophyte Trichophyton schoenleinii elaborates an extracellular collagenase which is specific for native collagen and gelatin and is separable from an enzyme which is active against Azocoll. The fungal enzyme differs from the bacterial collagenases in its acid pH and small molecular weight. In addition, the fungal enzyme is irreversibly inhibited by ethylenediaminetetraacetic acid, whereas the bacterial enzymes can be restored by divalent cations, such as Mg(++) and Ca(++).

Chromatography, Gel↗
PubMed1967

Elastase: production by ringworm fungi.

Isolants of nine species of Trichophyton, one of Epidermophyton, and four of Microsporum were assayed for elastase activity. The species or isolants with elastase activity were obtained from patients with inflammatory ring-worm infection. In Nannizzia fulva (M. fulvum), plus-mating-type strains were elastase-positive and minus-mating-type strains elastase-negative. A genetic study of mating type and elastase activity indicated a monogenic basis for both mating type and elastase activity.

Epidermophyton↗