PubMed Health⌕ Search

Biomedical subjects

J W Surgeon

Publications and source records attributed to J W Surgeon.

8 recordsLinked to original sources
PubMed1981

Subacute toxicity of intravenous dimethyl sulfoxide in rhesus monkeys.

Daily iv doses of 3 g/kg dimethyl sulfoxide in a 40% solution were given to rhesus monkeys for 9 consecutive days. The monkeys were monitored before and after treatment for 4 mo for changes in blood chemistry, hematology, urine, and ocular, neurological, and cardiovascular systems. At the end of the study all animals were sacrificed and gross and microscopic pathological examinations were performed. No significant or long-lasting changes were recorded in any of the parameters studied when these data were compared to those for saline controls.

Animals↗
PubMed1976

A methodological approach to rapid and sensitive monoamine histofluorescence using a modified glyoxylic acid technique: the SPG method.

A modified approach of the glyoxylic acid (GA) condensation reaction for the visualization of biogenic amines in tissue is described. Cryostat sections are used from brain or extracerebral tissue in dog, monkey, rat and mouse and exposed for 3 s to a room temperature solution containing sucrose-potassium phosphate-glyoxylic acid (SPG). The tissues are air dried and heated in an oven for 5 min. The complete precessing time from fresh tissue to microscopic examineation takes 18 min. Morphologically sharp and brightly fluorescent monoamine-containing neurons, pre- and terminal axons are seen against a dark parenchymal background without drug pre-treatment. The SPG method retains the high specific sensitivity for monoamines previously described in the original technique but is, in addition, more rapid and simple and is easily accissible as a research tool to investigators inexperienced in histofluoresecence techniques.

Animals↗
PubMedDate not supplied

Dexamethasone and DMSO in experimental transorbital cerebral infarction.

Twenty rhesus monkeys were used to evaluate the effects of dexamethasone and dimethyl sulfoxide (DMSO) following experimental occlusion of the middle cerebral artery (MCA) for 17 hours. Animals were initially treated after four hours while the MCA remained occluded and a series of tests was used to evaluate the neurological and cerebral status of each monkey for seven days. The results show that the gross, microscopic and angiographical picture of dexamethasone and no-treatment controls was practically identical. In contrast, DMSO-treated monkeys showed significant protection from the severe neurological deficits seen in the other groups. It is concluded that DMSO has a positive effect in reducing the neurological deficits seen in this model and may be potentially useful in clinical emoblic stroke.

Animals↗