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Biomedical subjects

J Waddell

Publications and source records attributed to J Waddell.

11 recordsLinked to original sources

Occupational stress and coping strategies among female baccalaureate nursing faculty.

Considerable stress is involved in nursing education. However, the sources and extent have not been fully investigated, nor have ways of coping. The main purpose of the study was to explore the sources and levels of perceived stress, coping strategies and effectiveness among female baccalaureate nursing faculty. The second purpose was to examine the relationship between stress, coping and academic responsibilities of the faculty member such as teaching, research and community service. A sample of 70 subjects from eight Ontario universities completed the demographic questionnaire, the researcher-developed 'Stress-Coping Anxiety Inventory', and the 'State-Trait Anxiety Inventory' Form Y (STAI) (Spielberger et al. 1983). Analysis of data using frequencies, cross tabulations, ANOVA and Pearson product-moment correlation were computed. Perceived stressors chosen most frequently as high in stress (greater than or equal to 4) (range = 1-5), in rank order, were 'heavy workload (combined clinical and classroom teaching)', 'retaining failing students', 'failing clinically unsatisfactory students', 'meeting research requirements' and 'providing individual clinical supervision'. Active coping strategies were selected more frequently as being effective (greater than or equal to 4) than passive coping strategies. Age of the respondent, number of years of full-time teaching and tenure status were most often significant factors (less than 0.05) relating to the level of stress. Implications for nursing education administrators and their faculty are discussed.

Adaptation, Psychological

The effects of motion on the healing of cancellous bone.

In 20 dogs olecranon osteotomies fixed with tension band wires were studied histologically to reveal the pattern of healing. Different degrees of stability existed in different areas of the osteotomy. Under stable conditions cancellous bone healed by direct formation of woven bone. Instability resulted in an internal cartilaginous callus. Once stability was restored, the callus underwent endochondral ossification. The articular defect, which was in an area of maximum instability, healed by fibrocartilage.

Animals

Electron microscopic localization of the chloroplast DNA replicative origins in Chlamydomonas reinhardii.

Chloroplast DNA, isolated from a synchronized culture of Chlamydomonas reinhardii, was digested with restriction endonucleases and examined in the electron microscope. Restriction fragments containing displacement loops (D-loop) were photographed and measured to determine the position of replicated sequences in relation to the restriction enzyme sites. D-loops were located at two positions on the physical map of chloroplast DNA. One replication origin was mapped at about 10 kb upstream of the 5' end of a 16s rRNA gene. The second origin was spaced 6. 5kb apart from the first origin and was about 16.5 kb upstream of the same 16s rRNA. Initiations at those two sites were not always synchronized. Replication initiated with the formation of a D-loop resulting from the synthesis of one daughter strand. After a short initial lag phase, corresponding to the synthesis of 350 +/- 130 bp of one daughter strand, DNA synthesis then proceeded in both directions. Both D-loop regions were preferred binding sites of undetermined protein complexes.

Base Sequence

Cloning and delimiting one chloroplast DNA replicative origin of Chlamydomonas.

The EcoR1 restriction fragments containing D-loops which marked the replication origin of chloroplast DNA were identified in two different species of Chlamydomonas. Each fragment was cloned in the E. coli plasmid pBR325. The cloned fragments were compared by restriction endonuclease analyses and by heteroduplex analyses in the electron microscope. The relative position of the D-loop regions and the homologous regions between the 2 fragments was determined. The D-loops were located within one short homologous region of 0. 42kb in length between the 2 cloned restriction fragments. The homologous region was subcloned in pBR322. Closed circular plasmid DNAs containing the short homologous region showed preferred denaturation in the D-loop region under physiological salt concentration which suggested that D-loop region was AT rich. Sequence divergence was detected at both ends of the D-loop region. Southern blot analyses indicated the presence of species-specific repetitive sequences within the divergent regions.

Base Sequence

Transmission electron microscopic study of polytene chromosome 2R from Drosophila melanogaster.

A simple and rapid method for studying polytene chromosome squashes by transmission electron microscope (TEM) is described. This technique provides close correlation between the light microscopic image and the TEM image. Fine structures of the chromosomes are preserved. The band pattern of region 44 A to 50 F of the chromosome 2 R has been analyzed and compared with Bridges' map (1935) and Lefevre's photographic representation (1976).

Animals

Thermal data from helicopters operating in a sub-arctic environment.

Using automatic and observer-operated equipment for monitoring thermal data, observations have been made during Royal Navy Wessex 5 helicopter operations in a sub-Arctic climate. Measurements were taken in flight of front and rear cabin temperatures and aircrew deep body and skin temperatures. The results indicate that the "arcticised" aircraft used had more than enough cabin heating to alleviate cold stress on the aircrew.

Aerospace Medicine

The below-the-knee amputation for vascular disease.

Of 190 consecutive patients with below-the-knee amputation done for diabetic or arteriosclerotic vascular disease, 167 were successfully fitted with a prosthesis and used the prosthesis in some or all of the activities of daily living. The surgical failure rate was 4.2 per cent; only eight patients required surgical revision to a higher level of amputation. The technique of rigid plaster dressing followed by delayed application of a plaster cast and pylon was not detrimental to wound healing and did not increase the interval between surgery and the use of the prosthesis, nor did it depress the eventual level of function. When compared with our own previous experience with other flaps, the long posterior flap offered a significant advantage in healing rate.

Amputation, Surgical