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Biomedical subjects

J Winther

Publications and source records attributed to J Winther.

26 records · Page 2Linked to original sources
PubMed1987

Characterization of an intraocular retinoblastoma-like tumour.

A transplantable intraocular retinoblastoma-like tumour growing in F 344 rat eyes and tissue cultures suitable for therapeutic experiments is characterized. The tumours grew in the vitreous and infiltrated the internal layers of the retina. Feeder vessels from the retina supplied the tumours. Few and often incomplete rosettes were seen, and calcification was sparse in areas of necrosis. Stainings for glial fibrillary acidic protein, S-100 protein, neuron-specific enolase and desmin were negative. Ultrastructural analysis brought out a close correspondence between the tumour cell and the human retinoblastoma and other virus-induced retinoblastoma-like tumours. The experimental tumour cells lacked cilia, but had neurosecretory granules. Chromosome analysis showed a modal chromosome number in the triploid range, and 6 marker chromosomes were demonstrated. Flow-cytometric analysis showed a S-phase cell fraction of 42%, and chromosome stability was suggested by a tumour-cell DNA index which remained stable for more than 3 months.

Animals↗
PubMed1987

Porphyrin photodynamic therapy in an experimental retinoblastoma model.

The effect of photodynamic therapy (PDT) with dihematoporphyrin ether and light irradiation (632.8 nm) was investigated in a fast growing intraocular rat retinoblastoma-like tumor. Complete remission was achieved in 15-25% of the tumors. Ocular side effects were: risk of intraocular hemorrhage and reversible cloudy cornea. The results suggest that PDT may be suitable for treatment of retinoblastoma, alone or in combination with radiotherapy, but further investigations are required before the PDT can be introduced in the clinic.

Animals↗
PubMed1986

In vitro and in vivo growth of an intraocular retinoblastoma-like tumour in F-344 rats.

Retinoblastoma-like cells grew in colonies on the bottom of tissue culture flasks. The population doubling time was 19 h. Tumour cells from cell cultures had a 39% plating efficiency, and fresh tumour cells from intraocular tumours had a 32% plating efficiency in colony forming assays. Inoculation of 1.5 X 10(4) tumour cells in the vitreous of F-344 rats resulted in a 100% tumour take and regularly growing tumours with a doubling time of 3 days. The tumour take-rate was not changed in wholebody immunosuppressed animals. The tumour volume was assessed under a stereo microscope, and it was possible to divide the tumours into 4 groups according to the number of intraocular tumour cells. Tumour growth caused eye perforation in 89% of the inoculated eyes. Spontaneous tumour regression was not seen in non-perforation groups. Immunosuppression with whole-body irradiation and dense traumatic cataract had no significant effect on the growth. It is concluded that this animal retinoblastoma-like tumour is suitable for quantitative therapy studies in vivo and in vitro.

Animals↗
PubMed1984

The combined effect of haematoporphyrin derivative and light on the normal mouse retina.

Combined treatment with haematoporphyrin derivative (Hpd) and light irradiation has been reported to be an effective method of killing cancer cells. This would be a very suitable treatment of intraocular tumours because the light can be controlled by the refractive media of the eye. Light and electron microscopy of Hpd and light treated normal mouse retina showed that only the irradiated area was damaged. Tissue destruction increased with greater concentrations of Hpd and longer light exposure. The outer layers of the retina were most sensitive. Hpd was retained in the tissue for 6 days.

Animals↗
PubMed1982

Dacryocystorhinostomy a.m. Iliff. A long-term follow-up.

Twenty-eight patients with dacryocystorhinostomies (DCR) a.m. Iliff were examined. 70% of the DCR in patients with idiopathic stenosis and 33% of the DCR in patients with post-traumatic stenosis had an open DCR. When the catheter was removed 3 weeks or later after surgery the success rates were 93% and 50%, respectively. It is concluded that DCR a.m. Iliff is a simple and reliable method.

Adolescent↗
PubMed1982

Central corneal thickness in patients with retinal detachment.

Central corneal thickness was measured in 20 patients with retinal detachment and in an age-matched control group. Central corneal thickness was significantly lower in the detachment eye and the contralateral eye. It is hypothetically suggested that the thin central cornea and the retinal detachment are ocular manifestations on a generalized connective tissue defect.

Adult↗