PubMed Health⌕ Search

Biomedical subjects

J Wysocki

Publications and source records attributed to J Wysocki.

At least 19 recordsLinked to original sources
PubMed1992

Effect of T cell activation on lymphocyte-endothelial cell adherence and the role of VLA-4 in the rat.

Lymphocyte migration from the blood is in part controlled by lymphocyte surface adhesion molecules, such as VLA-4 and LFA-1. Small lymph node lymphocytes from rats adhere poorly to rat microvascular endothelial cells (EC), while lymphoblasts from antigen-challenged lymph nodes have an enhanced adherence to EC and preferentially migrate to inflamed tissues. This lymphoblast adherence is partially inhibited by anti-VLA-4. The effects of in vitro activation of lymph node lymphocytes on lymphocyte-EC adhesion were examined. In vitro stimulation of T cells with concanavalin A or calcium ionophore and phorbol myristate acetate (PMA) for 1-4 hr caused a marked (7- to 12-fold) increase in lymphocyte adhesion to unstimulated and IFN-gamma- or LPS-treated EC. This adhesion was partially inhibited by anti-VLA-4, was not associated with increased VLA-4 expression, was partially inhibited at 4 degrees C, and was virtually eliminated at 4 degrees C with anti-VLA-4. Anti-CD3 or IL-2 stimulation of T cells also enhanced lymphocyte adhesion but required 2-3 days of culture. This adhesion was not inhibited by anti-VLA-4 and was almost totally inhibited at 4 degrees C, suggesting a primarily LFA-1-mediated adhesion. In conclusion, stimulation of T cells with Con A or calcium ionophore plus PMA caused a rapid enhancement of lymphocyte-EC adhesion mediated in part through VLA-4, while stimulation of T cells with anti-CD3 or IL-2 enhanced lymphocyte adhesion apparently independent of VLA-4.

Animals↗
PubMed1992

[Difficulties in diagnosing a case of typhoid fever].

A sporadic case of typhoid fever of 41 years of age male was characterized clinically mainly with long duration of fever and a few enlargement of liver. Diagnosis was made relatively late. In this case an endoscopic picture of upper gastrointestinal tract and ultrasonographic findings of the organs of abdominal cavity was described.

Abdomen↗
PubMed1992

The influence of thymus extracts on the chemotaxis of polymorphonuclear neutrophils (PMN) from patients with insulin-dependent diabetes mellitus (IDD).

The main goal of this study was to evaluate the possible influence of thymus extracts on the chemotaxis of PMN isolated from diabetic patients. The analyzed group consisted of fifty patients with insulin-dependent diabetes mellitus (IDD), and twenty healthy adults were taken as controls. The results showed, that PMN isolated from patients with IDD manifested the impaired chemotaxis toward zymosan-activated plasma (ZAP), plasma incubated with cellophane and supernate from E. coli culture. Both thymus extracts did not change the directed migration toward control plasma and toward ZAP. THYMEX-L did improve the migration of diabetic PMN toward plasma incubated with cellophane, whereas TFX-THYMOMODULIN ameliorated the impaired chemotaxis toward supernate from E. coli culture. Both thymus extracts can attract PMN obtained from healthy donors, but not the cells isolated from diabetic patients.

Adolescent↗
PubMed1987

Effect of organophosphoric pesticides on the behaviour of NBT-dye reduction and E rosette formation tests in human blood.

Investigations have been undertaken to determine the effect of occupational exposure to organophosphoric pesticides on the behaviour of NBT-dye reduction and spontaneous E rosette formation tests. A significant decrease of the values of the examined cellular parameters of human immunity and of the absolute lymphocyte count in peripheral blood was found in 31 workers, as compared to 30 controls. Moreover, the NBT-dye reduction decrease showed a positive linear correlation with a reduced acetylcholinesterase (AChE) activity. The exposure time showed a negative linear correlation of the phagocytic index of the NBT-dye reduction test. It is concluded that depressed NBT-dye reduction and diminished spontaneous E rosette formation may be regarded as a probable mode of the effect of organophosphoric chemicals on metabolic and membrane damage of human cells. A possible role of endogenous acetylcholine poisoning in the development of these pathologic conditions is also discussed.

Acetylcholinesterase↗
PubMed1985

A person-environment analysis of job stress: a contingency model explanation.

The contingency model of leadership was applied in a field study of job stress. Fifty-one university administrators completed a series of questionnaires that assessed their leadership style, degree of situational control within their work setting, perceived job stress, physical health, and psychological well-being. Multivariate analyses of variance (MANOVAs) showed that administrators whose leadership style and level of situational control were "in match" reported significantly less job stress, fewer health problems, and fewer days missed from work than administrators who were "out of match." The results are discussed as supporting the person-environment fit model of job stress.

Female↗
PubMed1985

[Serum levels of immunoglobulins and C 3 component of complement in persons occupationally exposed to chlorinated pesticides].

Serum concentrations of IgA, M, G and C-3 complement have been compared between 51 men occupationally exposed to chlorinated pesticides and 28 men of the control group. IgG has been found to be statistically significantly increased, whereas the concentration of IgM and C-3 complement--lower in the whole test group, as compared to the control group. These protein changes have been particularly strongly marked in the subgroup exposed to polycyclic chlorinated hydrocarbons. In the authors' opinion, the elevated IgG concentration in the occupationally exposed subgroup may result from immunostimulating effects of chlorinated pesticides, thus the decreased C-3 component of the complement in the serum in the test groups seems to be indicative of immune-complexes contribution to the pathogenesis of diseases resulting from the occupational exposure concerned. Furthermore, the authors suggest that the cyclic conformation of chlorinated pesticides may affect the pathomechanism of those diseases.

Adult↗
PubMed1983

The effect of mechanical vibration and industrial noise on the behaviour of blood serum immunoglobulins and on the lymphocyte blastic transformation.

The investigations have been undertaken to determine the effect of mechanical vibration on the behaviour of blood serum immunoglobulins and on the lymphocyte blastic transformation. The concentrations of IgA, IgG, IgM have been determined with 174 workers of metallurgical plants who were exposed to both vibration and noise, and with 50 workers exposed to noise only. The lymphocyte blastic transformation has been determined with 70 workers exposed to both vibration and noise. The investigations revealed that mechanical vibrations cause the concentration of IgA and IgG to increase, and that of IgM - to decrease; they also stimulate the lymphocyte blastic transformation. The noise itself causes the level of the examined immunoglobulins to lower.

Adult↗