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Biomedical subjects

J Yamada

Publications and source records attributed to J Yamada.

At least 469 records · Page 26Linked to original sources
PubMed1981

Long-term observation of canine brucellosis: excretion of Brucella canis into urine of infected male dogs.

Six male dogs orally inoculated with 3.2 X 10(8) Brucella canis and 2 male dogs naturally infected with the organisms were examined weekly for the presence of the organisms in urine and blood as well as for agglutinin titers in serum. Excretion of the organisms into urine started on 1 to 3 weeks after onset of bacteremia, i.e. 4 to 8 weeks after inoculation, and lasted for about 1 to 1.5 years. Non-bacteremic intervals, intermittent or lasting for about 1 to 6 months, followed the initial period of abundant excretion of viable organisms in urine. The highest urinary concentrations of the organisms, 2.5 X 10(4) to 1.5 X 10(6) cells per ml, were obtained in all dogs between the 6th and 14th weeks after the oral inoculation. Serum agglutinin titer rose on the 3rd to 5th weeks after the inoculation and then the titers remained at 1 : 640-1 : 2,560. The titers showed a downward trend over the 40 th to 56th weeks after the inoculation. In one of the spontaneously infected cases, the organisms were demonstrable only from urine for half a year with suspicious serum agglutinin titer of 1 : 160 after the bacteremic phase.

Agglutination Tests↗
PubMed1981

Segi's cap, a large aggregation of endocrine cells on the intestinal villi, occurs also in cattle and pig fetuses.

Segi's cap, a huge aggregation of basal-granulated cells on the top of the intestinal villi has been known only in human fetuses. This study reveals that the peculiar structure occurs also in cattle and pig fetuses. Segi's cap in these animals mainly consists of argyrophil endocrine cells and, in the cattle fetus, includes gastrin-, somatostatin-, motilin- and secretin-immunoreactive cells.

Animals↗
PubMed1980

Linkage analyses among five esterase loci in the laboratory rat (Rattus norvegicus).

A cluster of esterase loci has been identified on a segment of a rat linkage group V; however, the linear order of all the loci has not been established. We estimated the recombination frequencies of two locus combinations among five esterase loci (Es-1, Es-2, Es-3, Es-4, and Es-Si) and the linear order of the loci by using three sets of backcross matings: (1) (K:W x IS) x IS, (2) (K:W x IS) x IS, and (3) (SHR x W) x W). The linear order was determined to be ES-1-ES-4-ES-2-ES-3-ES-Si, although the order of ES-2 and Es-4 remains tentative. The sex-influenced esterase (Es-Si) was demonstrated to be distinct from Es-1 and was proposed to be Es-Si locus with two alleles of Es-Si(a) (positive) and Es-Si(b) (null).

Alleles↗
PubMed1980

Ultrastructure of avian gastrin cell granules.

Gastrin cells in the pigeon, quail, gull and kite were identified at ultrastructural level by immunocytochemistry, using the consecutive semithin/ultrathin section technique. In contrast to the gastrin granules known in mammals, the avian gastrin granules generally contained a consistent dense core accompanied by a clear halo between the core and limiting membrane. The mean diameters of gastrin granules in the pigeon, quail, gull and kite were 211 +/- 37, 247 +/- 45, 331 +/- 61 and 353 +/- 73 nm respectively. The carnivorous birds seemed to possess larger gastrin granules than the grain-eating birds. The species differences in the size and fine structures of the gastrin granules were discussed.

Animals↗
PubMed1979

Descending pathways of the nucleus of the optic tract in the rat.

The descending pathways of the nucleus of the optic tract (pars lateralis) (NOTL) in the rat were examined in Nauta-Gygax impregnated sections and further confirmed by the horseradish peroxidase labeling technique. Following stereotactic lesions in the NOTL, visual cortex, and superior colliculus, projections which orginate in the NOTL were identified by elimination of the tectofugal and tectopetal fibers of the superior colliculus. The descending fibers from the NOTL appeared to be organized into three bundles. (1) The NOTL-contralateral NOTL fibers, which connect bilateral NOTLs through a part of the posterior commissure. (2) The NOTL-nucleus tegmenti pontis and inferior olivary nucleus fibers, which project to the ventromedial part of the nucleus tegmenti pontis and the dorsal cap of the inferior olivary nucleus, ipsilaterally to the NOTL. (3) The NOTL-pontine nuclei fibers, which terminate within the medial one-third of the pars lateralis of the ipsilateral pontine nuclei with topical relations.

Animals↗
PubMed1979

Genetic variability within and between outbred Wistar strains of rats.

The allelic frequencies at 6 isozyme loci (Es-1, Es-2, Es-3, Es-4, Es-Si and Amy-1) were examined in 4 outbred Wistar strains and additionally 2 SD strains as controls. From the allelic frequencies, the averages of the heterozygosities in individual strains and the geometric genetic distances between every pair of all possible strain combinations were calculated. The averages of the heterozygosities in 2 SD strains were both intermediate (around 0.2) and the genetic distance between them was rather short. But among the Wistar strains, the averages of the heterozygosities were highly variable and the genetic relationships among them were very variable in their genetic distances. From these results, it was suggested that the outbred Wistar strains were different each other in their genetic constitutions and that no suggestion was obtained to descriminate genetically the Wistar strains from the SD strains.

Alleles↗
PubMed1979

Distribution and frequency of gastrin cells in the digestive tract of the Japanese quail.

The gastrin cells of the Japanese quail were studied histologically and immunocytochemically. Cells reacting with antiserum to gastrin (gastrin cells) were demonstrated by the peroxidase-labelled antibody method and showed brownish cytoplasm. They also were stained argyrophil by the Grimelius' silver method. Gastrin cells were found in the epithelium of the pyloric region and small intestine and not in any other regions. They were the most numerous in the pyloric region (382.14 +/- 12.77/1.25mm2), next in the ileum (3.79 +/- 1.24/1.25mm2) and duodenum (2.93 +/- 0.62/1.25mm2), and the least in the jejunum (0.93 +/- 0.62/1.25mm2). Remarkable concentration of gastrin cells in the pyloric region has thus been demonstrated.

Animals↗