Biomedical subjects
Publications and source records attributed to J Yelle.
Chromatin-associated DNA topoisomerase I activity was measured in human diploid fibroblasts during in vitro aging. No difference was detected as a function of cell age in the nicking and the closing activities of the DNA-unwinding enzyme. The capacity of type-I topoisomerase to relax superhelical DNA molecules was, however, increased in aged cells. An age-related increase in nucleoprotein content was also observed.
Standard or newly developed DNA transfection procedures were compared for both innocuity and efficiency using simian virus 40 (SV40) DNA and monkey kidney (Vero) cells as an indicator system. Optimal results have been obtained by treating the delicate cell monolayers with a solution of glycerol before infection with DNA-calcium phosphate complexes.
Viral deoxyribonucleic acid extracted from a limited number of cells infected with canine adenovirus type 1 or type 2 was cleaved with several restriction endonucleases. Agarose gel electrophoresis of the limit digests showed stable differences between the canine adenovirus type 1 and type 2 cleavage patterns. Rapid and accurate typing of large numbers of clinical isolates may thus be done by deoxyribonucleic acid restriction-endonuclease analysis.
Three types of virus-like particles (VLP) were observed in three of seven gerbil cell lines. One of these cultures was in a non-transformed state and contained R and C-type VLP, while the two others began to express R-type or intracisternal A-type VLP only after spontaneous transformation. These particles seem to be endogenous to the gerbil but do not appear to be directly involved in cell transformation.
A method is presented for the detection of virus-host interactions at the chromosomal level. This method relies on the analysis of recombinant plasmids carrying specific regions of a viral DNA molecule. Human cytomegalovirus HindIII subgenomic fragments were used here as a target for recombination events.