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Biomedical subjects

J Zambernard

Publications and source records attributed to J Zambernard.

At least 19 recordsLinked to original sources
PubMed1985

Clinical radiographs of the cadaver as a teaching aid in anatomy.

The use of clinical histories and radiographs of cadavers as a method for teaching anatomy was explored. This method was found to generate student interest in both anatomy and radiology, to make anatomy clinically relevant, and to supplement the laboratory manual by guiding the student through the dissection of medically or surgically complicated cases. It is an effective and workable method that is easily implemented and virtually cost free.

Anatomy↗
PubMed1985

Annular pancreas.

Explore the source record for details and available documents.

Adolescent↗
PubMed1983

Density and ultrastructure of mast cells in lung vessels of aging rats exposed to and recovering from chronic hypoxia.

A decrease in pulmonary vascular responsiveness in aging animals during exposure to chronic hypoxia has been previously reported; however, morphological documentation is lacking. Lungs from young (3-5 months) and aging (12-14 months) Sprague-Dawley rats, exposed to and recovering from chronic hypoxia, were morphometrically analyzed at the light-microscopic level for changes in perivascular mast cells, and at the electron-microscopic level for cellular alterations. While young rat lungs showed proliferation of mast cells around elastic and muscular pulmonary arteries and arterioles, perivascular mast cell density in lungs of aging rats was significantly greater than in young rat lungs. At the ultrastructural level, perivascular mast cells in aging hypoxic rats showed numerous profiles of cellular extensions that contained remnants of discharged secretory vesicles. The results suggest that increased proliferation of perivascular mast cells as well as increased secretory activity of vasoactive substances in aging animals might represent a humoral determinant of the hyporesponsiveness of pulmonary vessels that occurs with increasing age during chronic hypoxia.

Aging↗
PubMed1982

Fine structural changes in pulmonary veins of chronically hypoxic mice.

The pulmonary veins of male Swiss albino mice, exposed to simulated high altitude (380 mm Hg) for four weeks were studied for structural alterations. Endothelial cells contained invaginated nuclei and an accumulation of vesicles on the adluminal surfaces. In the subendothelial region, cells which contained filaments and dense bodies were noted. Increased muscular contraction, evidenced by decrease in the width of the I-band of the sarcomere, caveolae formation, sarcolemmal invaginations, and glycogen deposition were the most evident changes in the cardiac muscle layer. These alterations were interpreted as possible morphologically adaptive changes of pulmonary venous pulmonary vasoconstriction in response to increased metabolic demands as a result of changes in pulmonary hemodynamics with chronic hypoxia.

Altitude↗
PubMed1982

Glycogen localization in pulmonary vascular smooth muscle of chronically hypoxic rats.

The pulmonary trunk, muscular arteries, and arterioles of male Sprague-Dawley rats exposed to simulated high altitude hypoxia (380 mm Hg) for six weeks were studied for glycogen localization. As early as three days exposure time, glycogen particles were mobilized in the smooth muscle cells of muscular arteries and arterioles. Weekly sacrifice of animals showed increasing preferential accumulation of glycogen, near the sarcoplasmic reticulum, in the subsarcolemmal region, adjacent to micropinocytotic vesicles, and near the mitochondria in the smooth muscle cells of muscular arteries and arterioles. However, in the smooth muscle cells of the pulmonary trunk and newly muscularized vessels (arteries and arterioles), glycogen storage was not readily noted. These results suggests that the vascular energetics responsible for maintaining the pulmonary hypertensive state differ depending on the class of vessel. Also the muscular pulmonary arteries and arterioles which exhibited the greatest change in glycogen metabolism, may be primarily responsible for the maintenance of pulmonary hypertension.

Altitude↗
PubMed1982

Ultrastructural changes in rat alveolar macrophages exposed to oxides of copper and cadmium.

The morphological effects of single intratracheal injections of copper oxide (CuO) and cadmium oxide (CdO) (5 mg) on rat alveolar macrophages were studied. Rats sacrificed after a one week recovery period showed distinct morphological changes induced by CuO and CdO. Copper oxide induced hypertrophy of both the alveolar macrophages as well as the epithelial lining. The macrophages contained polymorphic nuclei with margination of the chromatin, crystalloid-like inclusions, concentric and parallel lamellar structures, lattice formationa and degenerative membranous structures. After CdO treatment, a slight increase in the number of alveolar macrophages and an attenuated thickening of the epithelial wall were noted. Two types of macrophages were identified: one type was large in size, with numerous vacuoles, electron dense aggregations, phagocytized mitochondria and fragmented chromatin. The other type was smaller in size, with fewer vacuoles, a distinct rough endoplasmic reticulum, mitochondria, and a highly convoluted plasma membrane. The results were suggestive of degenerative morphological alterations due to CuO and CdO treatments which were incompatible with normal macrophage function.

Animals↗
PubMed1982

Changes in pulmonary alveolar macrophages in rats exposed to oxides of zinc and nickel.

Pulmonary alveolar macrophages (PAM) from rats exposed to a single dose of ZnO or NiO showed distinct changes in size and ultrastructure. ZnO exposure brought about considerable reduction in the size of the cells, which can be distinguished into two types of macrophages. The macrophages contained a prominent nucleolus, several primary and secondary lysosomes, membrane formations, electron dense structures, and small dense mitochondria. In addition, electron dense and membrane bound structures were seen in the interstitial macrophages and in the intercellular spaces. NiO exposure induced hypertrophy and hyperplasia of the alveolar walls. The macrophages contained invaginated bizarre-shaped nuclei, pigment aggregations, and membrane bound structures. The results seem to suggest that after a one week recovery period following injections with ZnO and NiO, ZnO-related accumulations were transferred from alveolar to interstitial macrophages, however NiO-associated structures were contained within the alveolar macrophages.

Animals↗
PubMed1975

Fluorescent cytochemistry of calid and algid normal and Lucké tumor-bearing kidneys.

Fluorescent cytochemical techniques, chiefly end-group methods, have been employed in the characterization of cells in calid and algid normal and LUCKE tumor-bearing kidneys of the leopard frog, Rana pipiens. Cytoplasmic RNA and glycogen distribution reflect the known patterns of general metabolic activity of the respective cells in question. Protein sulfhydryl distribution is similar in normal distal tubules and tumors while both differ significantly from proximal tubules. Viral inclusions in nuclei of algid tumor cells contain demonstrable DNA, protein sulfhydryls and disulfides, arginine and lysine-rich basic proteins, also both C-terminal and side chain carboxyl groups. Significant amounts of DNA are demonstrable in the cytoplasm of algid tumors. Fluorescence cytochemical methods directed toward end-groups or specific classes of macromolecules offer a particularly favorable option, because of increased sensitivity and higher levels of contrast, for comparative cytological investigations of the LUCKE tumor and of similar systems.

Adenocarcinoma↗