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Biomedical subjects

J Ziaber

Publications and source records attributed to J Ziaber.

18 recordsLinked to original sources
PubMed2000

Increased expression of neutral endopeptidase (NEP) and aminopeptidase N (APN) on peripheral blood mononuclear cells in patients with multiple sclerosis.

We studied CD10 Ag of neutral endopeptidase (NEP) and CD13 Ag of aminopeptidase N (APN) expression on peripheral blood mononuclear cells (PBMC) as cells activation markers and for their transendothelial migration properties in three groups of MS patients (total 58); with acute exacerbation of MS (n = 18), primary progressive MS (n = 17) and with MS remission (n = 23). The control group (OND) consisted of 24 patients, suffering from other noninflammatory neurological diseases. CD10 Ag and CD13 Ag expression on PBMC was higher in clinically active MS (acute exacerbation and progressive MS) compared to MS remission and OND groups. Our study suggests that CD10 Ag and CD13 Ag can be useful mononuclear cell activation markers in the course of MS. CD13 Ag expression on PBMC may be also the sensitive marker of these cells transendothelial migration properties.

Adult↗
PubMed2000

Expression of aminopeptidase N (APN) on peripheral blood mononuclear cells' surface as a marker of these cells' transendothelial migration properties in the course of multiple sclerosis.

CD13 Ag and CD11a, CD11b, CD18 molecule expression on peripheral blood mononuclear cells (PBMC) were studied as these cells' adherent or transendothelial migration properties in three different multiple sclerosis (MS) patients groups (total 38): with clinically active MS (acute exacerbation of MS and primary chronic progressive MS (CP-MS)) and with MS remission. The control group consisted of patients, suffering from other non-inflammatory neurological diseases (OND). The results of our study suggest that CD11a/CD18 molecules expression on PB lymphocytes, although higher on these cells' surface in the course of MS as compared to OND, does not differentiate clinical forms of MS. CD11a molecule expression on monocytes did not differ significantly in all tested MS patient groups in comparison to OND. Although the expression of CD11b/CD18 molecules on monocytes' surface shows their activation in the course of MS, it does not differentiate them either. However, CD13 Ag of APN expression on PBMC surface may be an immunological marker of MS clinical form. CD13 Ag expression may also be a sensitive marker of these cells' transendothelial migration properties.

Adult↗
PubMed1998

The immunoregulatory abilities of polymorphonuclear neutrophils in the course of multiple sclerosis.

The polymorphonuclear neutrophils (PMN) possess sufficient potential to affect both immune response and inflammation, however it has not been yet described in the course of multiple sclerosis (MS). We have studied binding of fluorescein isothiocyanate (FITC)- stained TNF-alpha by PMN, the expression of CD11a, CD11b, and CD18 molecules of beta2-integrines and the expression of CD10 (neutral endopeptidase-NEP) and of CD13 (aminopeptidase N; APN) antigens on PMN in three different groups of MS patients. The control group included neurological patients (OND) with noninflammatory diseases. The obtained results have proved that during MS exacerbation and in the course of chronic progressive MS, PMN reveal several forms of preactivation, including significantly higher stained-TNF-alpha binding, higher expression of CD11b and CD18, as well as CD10 and CD13 antigens, in comparison with MS remission or OND. We suggest that the increased expression of these molecules on PMN of MS patients in exacerbation of the disease and to a lower degree in the course of CP-MS is a result of PMN priming, and directly prove the PMN involvement in the disease pathogenesis.

Adult↗
PubMed1997

Evaluation of myocardial muscle functional parameters in patients with multiple sclerosis.

Structural and functional parameters of myocardium were evaluated with 2D+ Doppler echocardiography in two similar age groups of MS patients: S1: 12 subjects in 3-4 Degrees EDSS (Expanded Disability Status Scale), S2: 12 subjects in 5-7 degrees EDSS. The control group comprised 12 healthy subjects temporarily (at least 1 month) immobilized due to lower limb fractures. Investigations were performed in the supine position and 3 min after tilting to the erect position. Symptoms of organic myocardial injury which might have caused its insufficiency were not observed in any subjects. All structural parameters evaluated by this method did not differ significantly in the examined groups. Symptoms suggesting myocardial insufficiency were found in patients from group S2. Statistically significant decrease of ejection fraction (EF) and cardiac output (CO) was observed in the supine position of S2 patients as compared to S1 and the controls. These symptoms intensified in the erect position in S2 patients and they were accompanied by the decreased values of stroke volume (SV). The fact that in the majority of patients orthostatic hypotonia was not observed and that those disorders were not compensated by significant intensification of heart rate suggest to us that besides disorders resulting from autonomic nervous system dysfunction they may have been caused by secondary myocardial injury in the course of MS.

Adult↗
PubMed1995

[The glossopharyngeal neuralgia with the coexisting cardiac rhythm disturbances].

We present the case of 61 years old man with idiopathic glossopharyngeal neuralgia. In the Holter's e.c.g often appeared the episodes of asystolia with the clinical symptoms of daze and confusion occurring during changing the position of the body (standing up). They were no accompanied by losses of consciousness. The patient was treated with carbamazepine during the cardiac disorders. The episodes of asystolia in e.c.g disappeared already 3 months after the end of the glossopharyngeal neuralgia.

Arrhythmia, Sinus↗
PubMedDate not supplied

[TNF-alpha binding ability as a sign of peripheral blood neutrophils preactivation in the course of multiple sclerosis].

Usually neglected is the role of neutrophils in causing of immunological disturbances in patients with multiple sclerosis (MS). Nevertheless, it has been indicated over the recent years that these cells possess a sufficient potential to affect both immune response and inflammation. This potential may result in MS through the process of priming of these cells by proinflammatory cytokines like TNF. We studied TNF and its soluble receptors sp55 and sp75 serum levels and binding of fluorescein isothiocyanate (FITC)-stained TNF by neutrophils. We studied three different groups of MS patients: 10 patients in relapse of the disease, 13 in its remission, and 11 in its chronic progressive form (CP-MS). The control was provided by 14 neurological patients (OND) with non-inflammatory diseases. The performed studies showed higher TNF sp55 and sp75 soluble TNF receptors serum levels in the patients with relapse, comparing with other MS patients and OND. TNF binding by neutrophils of MS patients during relapse was also higher, than other MS patients and OND. These result suggest the preactivation of neutrophils in the relapse of MS.

Adult↗
PubMedDate not supplied

[Increased expression of aminopeptidase N on lymphocytes in multiple sclerosis].

Aminopeptidase N is an ectoenzyme. Its expression on lymphocytes is the effect of cell activation. We studied APN expression on peripheral blood lymphocytes of multiple sclerosis (MS) patients and in the control group of patients with other neurological diseases (OND). We observed increased APN expression on lymphocytes of MS patients during acute exacerbation and in the course of chronic progressive MS compared to MS remission and OND groups. No such differences were found in CD11a molecule of LFA-1 integrin expression on lymphocytes. We suppose that APN expression on lymphocyte surface can be a sensitive marker of these cell activation in the course of MS.

Acute Disease↗
PubMedDate not supplied

Tumor necrosis factor-alpha binding by peripheral blood lymphocytes and polymorphonuclear neutrophils in patients with multiple sclerosis.

Tumor necrosis factor-alpha (TNF-alpha) in multiple sclerosis (MS) is responsible for peripheral blood leukocyte priming. The aim of this study was to evaluate fluorescein isothiocyanate (FITC)-labelled TNF binding ability by peripheral blood lymphocytes and polymorphonuclear neutrophils (PMNs) of MS patients, measured using flow cytometry (FACScan). Three groups of MS patients (total 34) were examined. Higher serum levels of TNF soluble receptors sp55 and sp75 were found in the MS patients during MS acute exacerbation (n = 10) and in chronic progressive forms (n = 11) as compared to MS remission (n = 13) and other neurological diseases (n = 14). Peripheral blood lymphocytes and PMNs of patients with acute exacerbation of MS bound TNF-FITC more effectively (p <0.01) as compared with the chronic progressive forms of MS, MS remission and other neurological diseases. The obtained results suggest a greater enhancement of TNF activity during MS acute exacerbation.

Adult↗
PubMedDate not supplied

[Increased percentage of "double phenotype" form T lymphocytes in blood of patients with multiple sclerosis].

We studied the percentage of double positive (CD4+CD8+) form of T-cells in a group (total 77) multiple sclerosis (MS) patients, measured by means of monoclonal antibodies anti-CD3, CD4/FITC, CD8/RPE and flow cytometry FACScan (Becton Dickinson). In our study we have shown that the percentage of the double positive T cells is higher (p < 0.05) in the peripheral blood of patients with acute exacerbation of MS (n = 21), and in the course of chronic progressive MS (n = 27) comparing to MS remission (n = 29) and other neurological diseases (n = 25) groups. In the study we have shown that the percentage of double positive T-cells in peripheral blood depends mainly on disease activity.

Adult↗
PubMedDate not supplied

[Acute and subacute autonomic neuropathy].

Acute and subacute autonomic neuropathies are rarely observed disease syndromes. Their symptoms result from the involvement of autonomic cholinergic fibres (cholinergic dys-autonomy) as well as cholinergic and noradrenergic ones (pan-dys-autonomy). The paper presents symptoms of both forms of the disease and methods of their diagnostics. Up-to-date trials of the treatment of orthostatic hypotonia a leading symptom of pan-dys-autonomy, have also been discussed.

Acute Disease↗
PubMedDate not supplied

[Calcification of the basal ganglia and cerebellum].

The basic clinical signs of calcifications of the basal ganglia and cerebellar dentate nuclei are discussed with separation of various forms of that disease. Particular attention was given to the factors which might predispose to the development of the so called idiopathic calcifications (Fahr syndrome), and secondary calcifications in various forms of parathyroid hypofunction.

Adolescent↗
PubMedDate not supplied

[Hormonal disturbances in patients with multiple sclerosis].

The results of certain tests for the relationship between the hormonal system and the immune system may shed some light on the aetiology of autoimmune diseases, including multiple sclerosis. In patients with that disease signs are found of various hormonal disturbances. It is supposed that they are caused mainly by hypothalamic lesions, but they may influence the course of the disease. The paper is trial of a broader presentation of these problems.

Autoimmune Diseases↗
PubMedDate not supplied

[Comparability of CT tomography and EEG in the evaluation of posttraumatic sequelae ].

Seventy-eight patients after craniocerebral trauma complicated with brain contusion, intracerebral or paracerebral haematoma, and multiple posttraumatic changes were subjected to control examinations for establishing the frequency of focal signs in neurological examination, in EEG and in psychological tests. The agreement was analysed also between the results of these examinations and those of control CT tomography 12-14 months after trauma. Not infrequently considerable divergences were noted between the obtained results of these examinations, particularly in patients after brain contusion.

Adolescent↗
PubMedDate not supplied

[Sequelae of craniocerebral traumas: CT tomography with digital image analysis].

Using the method digital analysis of CT image (ASOT programme) the cerebrospinal brain index was analysed in a group of 78 patients after past craniocerebral trauma. The studied subjects had had craniocerebral trauma complicated with focal brain contusions and intracerebral and paracerebral haematomas, as well as posttraumatic multiple changes. The control group comprised 12 subjects who had had cerebral concussion. A significant statistically increase of the index was found in the groups with past craniocerebral trauma complicated with intracerebral haematoma or multiple posttraumatic lesions.

Adolescent↗
PubMedDate not supplied

[Profiles of daily secretion of ACTH, beta-endorphins and cortisol in patients after minor craniocerebral trauma].

In 12 men aged 20-25 men who had minor craniocerebral trauma the profiles of daily secretion of ACTH, beta-endorphin and cortisol were studied. The studies were carried out on the second day after the trauma. Plasma concentrations of the hormones were determined on 8.00, 12.00, 17.00 and 22.00 hours. Statistically significantly increased, in relation to healthy persons, (p < 0.01) values of plasma hormone concentration were found at all time points. In 50% of the patients (six cases) a disturbance was found of the profile of daily secretion of ACTH and beta-endorphin. The disturbance of the profile of cortisol secretion was found only in one patient.

Adrenocorticotropic Hormone↗