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Biomedical subjects

Jacek Rózański

Publications and source records attributed to Jacek Rózański.

At least 19 recordsLinked to original sources
PubMed2006

Peripheral blood lymphocytes P-glycoprotein (P-gp, gp-170) expression in allogenic kidney transplant patients.

AIM AND METHODS: P-glycoprotein (gp-170, P-gp) is a transmembrane transporter involved in drug, for example cyclosporine A, efflux from the cells thus limiting their intracellular concentration. Expression of the transporter on the surface of immune competent cells may be associated with poor prognosis in kidney transplant patients. The aim of the present study was to evaluate P-gp expression on the surface of CD4(+), CD8(+), CD19(+) and CD56(+) cells in kidney transplant patients treated with cyclosporine A as a main immunosuppressant, using flow cytometry. RESULTS: It was found that P-gp expression in kidney transplant patients with acute rejection did not differ significantly from transplanted patients without rejection studied in the same period after transplantation, as well as from the healthy controls. Administration of 3-day course of 1,000 mg/24 h methylprednisolone did not affect the expression of P-gp in the studied cells, except for significant elevation in CD56(+) cells, which disappeared at 2 weeks after cessation of steroid administration. CONCLUSION: Based on the results from the present study it can be concluded that P-gp expression is not a prognostic factor of acute kidney graft rejection.

ATP Binding Cassette Transporter, Subfamily B, Mem↗
PubMed2006

Early phase of reperfusion of human kidney allograft does not affect an erythrocyte anti-oxidative system.

BACKGROUND: Generation of reactive oxygen specimens is the basic mechanism leading to ischaemia/reperfusion injury of the kidney graft. Oxygen burst is a trigger for sophisticated biochemical changes leading to generation of oxygenated lipids and changes in microcirculation, which recruit recipient's neutrophils and contribute to delayed graft function. It has been shown that the free radicals generation correlates with the activity of anti-oxidative system. Superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione (GSH) are involved in protection against free radicals. AIM: To examine the activity of erythrocyte anti-oxidative system during reperfusion of the transplanted kidney allograft. METHODS: The study included 40 renal transplant recipients. Blood was taken from the iliac vein before transplantation and from the graft's renal vein immediately, as well as 2 and 4 min after total reperfusion. The authors assessed the process of reperfusion using ThermaCAM SC500 termovision camera. Spectrophotometric methods were used to measure superoxide dismutase, glutathione peroxidase and catalase activity as well as glutathione concentrations in erythrocytes. RESULTS: There were no statistically significant differences in the activities of superoxide dismutase, catalase and glutathione peroxidase as well as glutathione concentrations during the first 4 min after total graft reperfusion. Nevertheless, there was a positive correlation between the activity of superoxide dismutase and glutathione peroxidase. CONCLUSION: The results suggest that the erythrocyte anti-oxidative system is stable during the early phase after reperfusion. An association between some anti-oxidative enzymes was noted.

Antioxidants↗
PubMed2006

[Ablation of incisional right atrial tachycardia with critical region between surgical scar and crista terminalis in a patient with corrected transposition of great arteries after correction of Fallot-like pentalogy, Ebstein-like tricuspid valve anomaly and WPW syndrome].

In a 14 year old boy with corrected transposition of great arteries after correction of Fallot-like pentalogy, Ebstein-like tricuspid valve anomaly and WPW syndrome that was treated surgically, atrial tachycardia (AT) 260-280 ms was diagnosed and ablated. Due to earlier therapy it was impossible to insert catheters through femoral veins, and a subclavian and carotid veins access was used. The arrhythmia was a peri-incisional right AT. The isthmus was diagnosed with the use of entrainment pacing between a scar near VCI and the surgical incision along crista terminalis. Application in that region terminated AT and it was not inducible after the procedure.

Adolescent↗
PubMed2005

The relationship between blood pressure, pulse pressure and right ventricular function following an atrial switch procedure for complete transposition of the great arteries.

BACKGROUND: The pressure overload may be responsible for the failure of the systemic ventricle. No study so far has evaluated the association between arterial blood pressure values and right ventricular function in adults with atrially corrected complete transposition. METHODS: This was a retrospective analysis. The studied population consisted of 60 patients with complete transposition, 11.5+/-2.7 years after atrial switch procedure, divided into subgroups according to the severity of systemic ventricular dysfunction (right ventricular ejection fraction < or = 0.40 vs. > 0.40), and the severity of perfusion abnormalities in the radionuclide study (absent or mild vs. moderate-to-severe). RESULTS: All patients had blood pressure values (systolic 109.1+/-11.7 mm Hg and diastolic 72.3+/-9.7 mm Hg) within the normal range. Systolic blood pressure values correlated inversely with right ventricular ejection fraction (r = -0.450; p < 0.001). Compared to patients with systolic blood pressure below median values, patients with "elevated" blood pressure had lower right ventricular ejection fraction (32.6+/-6.3 vs. 38.9.+/-7.2; p < or = 0.002) and more significant perfusion abnormalities (1.5+/-1.0 vs. 2.9+/-1.5; p < or = 0.001). In a multivariate backward logistic regression model age at surgery and at the time of the study, systolic blood pressure at rest predicted impaired right ventricular ejection fraction (p < 0.02). Greater pulse pressure at peak exercise female sex were associated with greater more severe perfusion abnormalities (p < 0.01). CONCLUSIONS: There is a significant correlation between blood pressure values and indices of right ventricular dysfunction in patients who underwent an atrial switch procedure for complete transposition. Blood pressure values might be considered as a surrogate end point in these patients.

Adolescent↗
PubMed2005

Detachment of the right coronary artery resulting in subsequent aneurysm of the ascending aorta and pulmonary artery fistula 7 years after a bentall procedure.

This report describes detachment of the right coronary artery, an extremely infrequent late complication of Bentall's procedure combined with Cabrol's maneuver for aneurysm of the ascending aorta. This resulted in subsequent formation of the progressive aneurysm of the ascending aorta and fistula entering the main pulmonary artery. Successful surgical treatment depended on meticulous echocardiographic investigation and careful physical examination.

Adult↗
PubMed2005

[Resistance to activated protein C in a patient with multiple myocardial infarctions and stroke--a case report].

A case of a 49-year-old female with a history of two myocardial infarctions (MI) and ischaemic stroke is presented. The patient was admitted to the hospital due to a third acute MI. Laboratory investigations revealed resistance to activated protein C due to factor V Leiden mutation. Diagnosis and treatment of patients with this condition are discussed.

Activated Protein C Resistance↗
PubMed2005

Cardiac amyloidosis diagnosed by endomyocardial biopsy. Clinical, histopathological, immunohistochemical and ultrastructural studies.

BACKGROUND: The heart is often involved by primary (AL) and familial transthyretin-related (ATTR) amyloidosis. Endomyocardial biopsy is a valuable diagnostic method, useful in detection and recognition of the type of amyloid. AIM: The aim of the study was to determine the type of amyloid deposits found in endomyocardial biopsies, using histochemical, immunohistochemical and ultrastructural methods. The correlations between morphological and clinical parameters were evaluated. METHODS AND RESULTS: During 1999-2004, endomyocardial biopsy was performed in 41 patients hospitalized in our institution due to heart failure of unknown etiology. Amyloid deposits were revealed in 3 patients, in 2 of them the intraoperative specimens were also taken. The deposits were located in myocardial interstitium as well as in the wall of small vessels. One patient showed deposits in the atrial and pericardial walls. Electron microscopy study revealed amyloid fibers adhering to the basement membranes of the myocardial cells and small vessels. Immunohistochemical staining determined two types of amyloid. A woman aged 42 showed transthyretin-related amyloidosis, most probably hereditary. A woman aged 69 exhibited the light chains amyloidosis (AL-lambda). In contrast, diagnosis was not certain (most probably ATTR) in a 35 year old woman. Essential involvement of the pericardium was observed in two younger patients. They underwent pericardiotomy. The survival time (from diagnosis to death) was one year in the patient with amyloidosis ATTR, 17 days in the patient with the AL type, and 2 years in the patient with amyloidosis of undetermined type. CONCLUSIONS: Cardiac amyloidosis is associated with poor prognosis. Better recognition of this disease may allow the early diagnosis and institution of modern therapy.

Adult↗
PubMed2005

[Anomalous pulmonary artery as a cause of wide, polycyclic pulmonary hilus--case report].

Changes of lymphatic system, vascular disease during pulmonary hypertension or aneurysms are very often considered to be a cause of wide pulmonary hilus. However, we should remember developmental anomaly of pulmonary artery, too. We present a case of a patient hospitalized very often due to necessity of diagnostic wide, polycyclic pulmonary hilus. Only digital subtraction angiography allowed us to know the reason of wide pulmonary hilus as the developmental anomaly of pulmonary artery.

Angiography, Digital Subtraction↗
PubMed2005

[Quality of life in patients after the Ross operation for aortic stenosis].

The pulmonary autograft provides ideal conditions regarding hemodynamics. Its additional value is durability and low thromboembolic hazard. We postulated that the Ross technique results in minimal restrictions on patient's lifestyle as compared to normal controls and we expected the patients to have good quality of life (QoL). On the other hand QoL may be affected by the risk of the homograft degeneration and the need of reoperation in the future. The aim of the study was to assess the QoL of patients after Ross operation because of aortic valve stenosis. Postoperative condition of the patients was judged by resting transthoracic echo, 24 hour ECG recording, maximal exercise test on bicycle ergometer. The mean age of patients was 31.1 (N=50; 18 female, 32 male). The mean follow-up was 3.6 years. We applied the Short Form Health Survey Questionnaire (SF-36) for assessment of QoL. The results of psychological tests were compared with age, gender and education matched control group without underlying cardiac disease. Overall QoL (QoL index) did not vary between patients and control group. The only significant difference concerned physical sum score and subtests of physical functioning (scale 1) and general health perception (scale 8). There were no differences in psychological health sum score. We concluded that in general, patients after the Ross operation enjoy a normal life style. The results of our study are consistent with well known advantages of Ross procedure. This study also implies the need for more detailed information concerning patient's lifestyle after the operation. Patient's family and doctors who are to take care of the patient after leaving hospital ought to be instructed as well. Education should specify patient's ability to perform different everyday abilities, especially physical ones. The aim of the education is to eliminate negative self-assessment of patient's own physical abilities which probably originate from preoperative period and stress connected with operation.

Adolescent↗
PubMed2004

[Tuberous sclerosis--symptoms, diagnosis and treatment].

Tuberous sclerosis (Bourneville-Pringle disease) is an inherited disease with a prevalence rate ranging from 1:10,000 to 1:23,000. It is inherited as an autosomal dominant with a variable gene penetrance. However about 60% of cases represent new mutations. This disease is characterized by a defect in cell migration, proliferation and differentiation in organs like skin, brain, kidneys, heart, lungs and eyes. The mechanism involves formation of hamartoma tumours responsible for the functional impairment of these organs.

Humans↗
PubMed2004

[Vascular malformations].

Aneurysms, hemangiomas, congenital arteriovascular and lymphatic fistulas often formed as a result of genetically determined defects in the vessel wall structure. They contribute to the development of sysytemic diseases like hypertension or circulatory failure. The author concentrates on the definition of malformation and its types, the review of clinical studies and guidelines for diagnosis and treatment.

Aneurysm↗
PubMed2004

[Mitochondrial syndrome].

Mitochondrial syndrome is a metabolic disease, caused by punctiform mutations or depletions in nuclear and mitochondrial DNA. These genetic lesions alter mitochondrial oxidative phosphorylation, what leads to reduction in energy produced for cell activity. Particular severity of symptoms can be observed in organs and tissues highly dependent on oxygen metabolism. Symptoms can be seen in central and peripheral nervous system, skeleton muscles, pancreas, endocrine glands, kidneys and gastrointestinal tract. Diagnostics is difficult and expensive. It is based mainly on genetical assays and respiratory chain investigations in bioptate of skeleton muscles. So far the treatment is based on decreasing symptoms intensity. Presently studies are being conducted on gene treatment of this disorder, which aim is to provide evidence for efficacy of using respiratory chain co-factors in the treatment of mitochondrial syndrome.

DNA, Mitochondrial↗