PubMed Health⌕ Search

Biomedical subjects

Jacek Szamatowicz

Publications and source records attributed to Jacek Szamatowicz.

6 recordsLinked to original sources
PubMed2005

Matrix metalloproteinase-13 and membrane type-1 matrix metalloproteinase in peritoneal fluid of women with endometriosis.

OBJECTIVE: It is becoming more and more evident that different types of matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) are involved in the pathogenesis of endometriosis. The aim of the present study was to measure levels of the active forms of MMP-13 and membrane type-1 matrix metalloproteinase (MT1-MMP)/MMP-14 as well as TIMP-2 in the peritoneal fluid of women with endometriosis. STUDY DESIGN: We determined the levels of the active forms MMP-13 and MT1-MMP/MMP-14 as well as TIMP-2 in the peritoneal fluid of 20 women with endometriosis and 18 controls by different types of enzyme-linked immunosorbent assay. RESULTS: We found that the concentrations (mean +/- standard deviation) of total active MMP-13 and endogenous active MT1-MMP/MMP-14 in the peritoneal fluid of patients with endometriosis were 1.69 +/- 0.67 and 3.12 +/- 1.07 ng/ml, respectively. In control women the corresponding values were 3.02 +/- 0.43 and 4.45 +/- 1.03 ng/ml. The differences were statistically significant (p < 0.0001 and p < 0.0004 for MMP-13 and MMP-14, respectively). Levels of TIMP-2 did not differ significantly. CONCLUSIONS: Decreased concentrations of active MMP-13 and MT1-MMP/MMP-14 may imply that the proteolytic activity of the peritoneal milieu of women with endometriosis is disturbed, which may have implications in the pathogenesis of the disease.

Adult↗
PubMed2005

[Contemporary methods of treating age-related infertility].

Age-related infertility that is caused by diminished reproductive potential is both medical and social problem of a great concern. Changes in modern ways of life make women postpone the decision of starting up a family, what may reduce their chances of having a healthy baby. It is also a problem of subfertile couples who after many years of insufficient treating approach the period where the age imposes on primary causes of infertility, what even more reduces the chance of pregnancy. The main reason of this is increased number of chromosomal abnormalities resulting in lower quality of oocytes. There is a lot of research being held on the reconstruction of gametes by transferring the nucleus or cytoplasm among the oocytes derived from young and older patients. However, it has not brought the expected results so far. The method of preimplantation genetic diagnosis--aneuploidy screening (PGD-AS) in patients older than 35 gives some hope for improving the efficiency of infertility treatment. Nevertheless, oocyte donation still remains the most effective and applied method of assisted reproduction in the group of older patients.

Adult↗
PubMed2004

Cystic fibrosis as a cause of infertility.

Cystic fibrosis (CF) is one of the autosomal recessive diseases, caused by mutations in a gene known as cystic fibrosis transmembrane regulator (CFTR). The majority of adult males with CF (99%) is characterized by congenital bilateral absence of vas deferens (CBAVD). CBAVD is encountered in 1-2% of infertile males without CF. Females with CF are found to be less fertile than normal healthy women. In females with CF, delayed puberty and amenorrhoea are common due to malnutrition. CFTR mutations are also associated with congenital absence of the uterus and vagina (CAUV). The National Institutes of Health recommend genetic counseling for any couple seeking assisted reproductive techniques with a CF male or obstructive azoospermia which is positive for a CF mutation.

Cystic Fibrosis↗
PubMed2002

[Two treatment methods of infertility associated with polycystic ovarian syndrome].

OBJECTIVES: 30 patients treated for infertility associated with PCO were analyzed. Anovulatory cycles and US scans of ovaries were taken as criteria for PCO. MATERIALS AND METHODS: Patients were treated either by the laparoscopic electrocauterization (n = 16) of ovaries or by the ultrasonographic punction of follicles (n = 14). Number of ovulatory cycles, cumulative pregnancy rate and hormonal changes were analyzed. RESULTS: We observed the same efficacy of both methods in terms of ovulatory cycles and pregnancy rates. The cumulative pregnancy rate was 37.5% in laparoscopic and 35.7% in ultrasonographic groups during the 3 months' observation. CONCLUSIONS: Ultrasonographic punction has appeared to be easier to do and safer for the patient and have the same pregnancy rate. It also gives opportunity for in vitro culture and maturation of obtained oocytes.

Adult↗