Biomedical subjects

Jae Ho Shin

Publications and source records attributed to Jae Ho Shin.

8 recordsLinked to original sources

PubMed2006

Poly(vinylpyrrolidone)-doped nitric oxide-releasing xerogels as glucose biosensor membranes.

Nitric oxide (NO)-releasing xerogel membranes were prepared as coatings for an electrochemical glucose biosensor to allow for enhanced biocompatibility while maintaining adequate response times and sensitivity. Formation of the NO-donor species was found to drastically decrease the permeability of the aminosilane-based xerogels to both hydrogen peroxide and glucose. The addition of poly(vinylpyrrolidone) (PVP) polymer enhanced the membrane permeability even after exposure to high pressures of NO (necessary for NO-donor synthesis). The analytical response and NO release of PVP-doped NO-releasing xerogels as glucose sensor membranes were further investigated and found to be enhanced via polymer doping. Doping of the polymer into the xerogel did not compromise the stability of the xerogel as evaluated by silicon leaching studies. Despite the addition of PVP, the NO-releasing xerogels maintained reduced bacterial adhesion characteristics analogous to previous reports for NO-releasing xerogels.

Biosensing Techniques↗

PubMed2006

Improving the biocompatibility of in vivo sensors via nitric oxide release.

The continuous, real-time monitoring of clinically important analytes (e.g., PO2, PCO2, pH, K+, Na+, glucose, and lactate) is of great importance to human health care. Despite considerable efforts spanning several decades, the use of in vivo sensors clinically remains limited due to inadequate biocompatibility. The discovery of nitric oxide (NO) as an effective inhibitor of platelet and bacterial adhesion has opened a new direction of research related to designing the next generation of in vivo sensors. In this Highlight article, recent progress in designing more biocompatible in vivo sensors is described, with a particular focus on preparing interfaces that resist biofouling via controlled NO release.

Bacterial Adhesion↗

PubMed2005

Sol-gel derived amperometric nitric oxide microsensor.

An amperometric sol-gel derived nitric oxide microsensor is described. Several silicon-based xerogel membranes are evaluated to identify the optimum composition for maximizing NO permeability while providing sufficient selectivity for NO in the presence of common interfering species. Xerogel permeability and selectivity are further manipulated as a function of reaction/processing conditions. In addition, the effects of incorporating Nafion into the xerogel matrix on sensor performance and the stability of the ensuing xerogel/Nafion hybrid film are evaluated. The optimal permselective membrane is achieved by catalyzing polycondensation of the xerogel composed of methyltrimethoxysilane and (aminoethylaminomethyl)phenethyltrimethoxysilane and Nafion with NO gas. The resulting NO microsensor exhibits a sensitivity of 0.17 +/-0.02 pA/nM (from 25 to 800 nM, r = 0.9991), detection limit of 25 nM (S/N = 3), response time of 9 s (t(95%), a NO concentration change from 400 to 500 nM), selectivity (log K(NOj) amp) of -5.8, <-6, <-6, and <-6 for j = nitrite, ascorbic acid, uric acid, and acetaminophen, and a lifetime of 8 d (82% of initial sensitivity without serious deterioration in selectivity).

Electrochemistry↗

PubMed2004

Influence of surfactants and antibody immobilization strategy on reducing nonspecific protein interactions for molecular recognition force microscopy.

Specific and nonspecific interactions between antibody-modified probes and substrate-immobilized proteins were monitored by atomic force microscopy (AFM). Probes were modified with anti-ovalbumin IgG antibodies immobilized in either an oriented or a random manner. The oriented immobilization of whole IgG was accomplished through the use of Protein A, and random immobilization was carried out with glutaraldehyde. Nonspecific interactions may lead to false detection of antibody-antigen binding events even when the antigen binding sites are properly positioned by an oriented immobilization strategy. Thus, nonionic and zwitterionic surfactants, including Tween 20, Tween 80, Triton X-100, and CHAPS, were evaluated to determine if nonspecific binding events could be reduced without compromising the desired specific antibody-antigen binding. Enzyme-linked immunosorbent assay and surface plasmon resonance assays were also employed to study antibody-antigen binding as a function of immobilization strategy and surfactant concentration. The data from these studies indicate that Protein A can be used to immobilize whole IgG onto AFM probes for force measurement experiments and that a surfactant is useful for improving the selectivity for such measurements.

Antibodies↗

PubMed2004

Nitric oxide-releasing sol-gel particle/polyurethane glucose biosensors.

A hybrid sol-gel/polyurethane glucose biosensor that releases nitric oxide is developed and characterized. The biosensor consists of a platinum electrode coated with four polymeric membranes including the following: (1) sol-gel with immobilized glucose oxidase (GOx); (2) polyurethane to protect the enzyme; (3) NO donor-modified sol-gel particle-doped polyurethane; and (4) polyurethane. This configuration was developed due to the drastic reduction in sensitivity observed for NO donor-modified sol-gel film-based glucose sensors. For the hybrid sol-gel/polyurethane biosensor, sol-gel particles are first modified with the NO donor and then incorporated into a polyurethane layer that is coated onto the preimmobilized GOx electrode. In this manner, the GOx layer is not exposed to the harsh conditions necessary to impart NO release ability to the biosensor, and only a minimal decrease in sensitivity due to the NO release is observed. The glucose response of the NO-releasing glucose biosensor and its NO generation profiles are reported. In addition, the stability of the sol-gel particles in the supporting polyurethane membrane is discussed.

Biosensing Techniques↗

PubMed2004

Characterization of epoxy resin-based anion-responsive polymers: applicability to chloride sensing in physiological samples.

The potentiometric properties of an epoxy resin-based anion-responsive polymer and its feasibility as a chloride sensing membrane are described. The response mechanism of the epoxy resin-based membrane is studied by varying the types and contents of epoxy components (e.g., resin, hardener, diluent, and plasticizer). It is found that epoxy resins cured with polyamine-type hardeners have excellent characteristics as chloride-sensing membranes, in terms of their selectivity, stability, sensor lifetime, and adhesion for constructing an all-solid-state sensing device. In addition, effects of the diluent or plasticizer added in an epoxy resin/hardener system on the potentiometric behaviors are investigated. To demonstrate the practical analytical utility of the epoxy resin-based electrodes, chloride levels in various controls and biological specimens (e.g., human sera and whole blood) are determined with a flow-cell system.

Journal Article↗

PubMed2004

Assessing estrogenic activity of pyrethroid insecticides using in vitro combination assays.

Pyrethroid insecticides are among the most commonly used classes of insecticides worldwide, but their endocrine disrupting activities remain unclear. Therefore, in the present study, we examined the estrogenic activities of pyrethroid insecticides in E-screen and competition binding assays. In addition, we measured estrogen receptor (ER) protein and pS2 mRNA levels in human breast cancer cells (MCF-7 BUS) to clarify the mechanism of their estrogenicity. Seven pyrethroid insecticides (bioallethrine, cypermethrin, deltamethrin, fenvalerate, permethrin, sumithrin, and tetramethrin) were tested because of their worldwide usage. In addition, 17beta-estradiol was tested as a positive control. As expected, 17beta-estradiol significantly increased MCF-7 BUS cell proliferation at concentrations of 10(-11) M and above. Of the pyrethroid insecticides tested, only sumithrin increased MCF-7 BUS cell proliferation in a dose-dependent manner; the maximum induction of cell proliferation was observed at a dose of 10(-5) M. In the anti-estrogenic activity test, bioallethrin, fenvalerate, and permethrin significantly inhibited 17beta-estradiol-induced MCF-7 BUS cell proliferation at 10(-6) M, a concentration comparable to the effective dose (10(-9) M) of ICI 182,780, a pure ER antagonist. However, none of the pyrethroid insecticides competitively inhibited the binding of [(3)H]estradiol to rat uterus ERs in competition binding assays. Both 17beta-estradiol (10(-10) M) and sumithrin (10(-5) M) decreased the levels of cytosolic ERalpha and ERbeta protein expression significantly as compared with the vehicle control. In addition, 17beta-estradiol (10(-10) M) increased pS2 mRNA expression markedly, and sumithrin significantly increased pS2 mRNA levels in a dose-dependent manner. The other six compounds tested in the present study did not affect ER protein levels or pS2 mRNA levels. These results suggest that certain pyrethroid insecticides may be considered to be estrogen-like chemicals that act through pathways other than direct ER binding, and may function as endocrine modulators in both wildlife and humans.

Animals↗

PubMed2002

Determination of oceanic carbon dioxide using a carbonate-selective electrode.

Potentiometric properties of the PVC membrane-based electrodes prepared with molecular tweezer-type neutral carriers, 3,12-bis(TFAB)CA and deoxy-3,12-bis(TFAB)CA, and trifluoroacetyl-p-decylbenzene (TFADB) were measured in buffered electrolytes (0.1 M Tris-H2SO4, pH 8.6 and 8.0) and artificial seawater. It was observed that the deoxy-3,12-bis(TFAB)CA-based electrode provides greatly enhanced carbonate selectivity over chloride (log K(CO3(2-), Cl-)POT approximately -6) and other minor anions present in seawater. Thus, we explored the possibility of applying this new carbonate-selective electrode for direct determination of oceanic carbon dioxide. The total carbon dioxide (TCO2) level in surface Yellow Sea water was determined with the deoxy-3,12-bis(TFAB)CA-based electrode, Severinghaus-type CO2 gas sensor, and the traditional potentiometric titration methods. The results showed that the carbonate-selective electrode provides accurate oceanic TCO2 determination comparable to that obtainable with the other two methods. The analytical procedure based on a carbonate-selective electrode is clearly advantageous over other conventional methods: it does not require any sample pretreatment and extra reagents other than the standard calibration solutions, while providing the measured results directly and immediately.

Carbon Dioxide↗