Biomedical subjects

Jae-Il Lee

Publications and source records attributed to Jae-Il Lee.

9 recordsLinked to original sources

PubMed2006

Relationship between two-dimensional and three-dimensional bone architecture in predicting the mechanical strength of the pig mandible.

OBJECTIVES: To investigate the relationship between two-dimensional (2D) and three-dimensional (3D) bone imaging parameters. STUDY DESIGN: Bone specimens were obtained from the mandibles of five male pigs weighing around 110 kg each. A total of 111 samples were measured two-dimensionally with using solid state digital intraoral radiography. Of these 111 samples, 43 were selected for 3D analysis and measured by microcomputed tomography. Through destructive mechanical testing, strength parameters were obtained. RESULTS: Correlations between the 2D and 3D parameters were rare; however, both 2D and 3D parameters separately showed significant correlations with strength. Multiple linear regression analyses using both 2D and 3D parameters together showed greater predictability than those using only 2D or only 3D parameters. CONCLUSION: Architectural parameters in 2D and 3D independently affect trabecular strength; the combination of the two can be used to improve bone strength predictability.

Animals↗

PubMed2006

Oligonucleotide microarray analysis of ameloblastoma compared with dentigerous cyst.

BACKGROUND: Ameloblastoma is a benign, but locally invasive tumor known for its high rate of recurrence. However, few comprehensive genetic studies have been conducted about its tumorigenesis. Our aim was to identify possible genes involved in the development and progression of ameloblastoma, using microarray analysis with dentigerous cyst as a control. METHODS: Total RNA was extracted from two fresh dentigerous cysts and ameloblastoma specimens. Following microarray analysis, semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry were performed on selected genes. RESULTS: Seventy-three genes were overexpressed and 49 were underexpressed. These genes were divided into categories according to function. The microarray results for 13 selected genes were verified with semiquantitative RT-PCR. CONCLUSIONS: We identified important genes related to the development and progression of ameloblastoma through a large-scale gene expression analysis. This study will stimulate further investigations on genes significant for early diagnosis and prognosis of ameloblastoma.

Adolescent↗

PubMed2006

Serodiagnostic comparison of enzyme-linked immunosorbent assay and surface plasmon resonance for the detection of antibody to porcine circovirus type 2.

This paper describes the cloning and expression of the capsid protein of Porcine circovirus type 2 (PCV2) in an Escherichia coli expression system that was used to produce a fusion protein for subsequent immunologic studies: enzyme-linked immunosorbent assay (ELISA) and surface plasmon resonance (SPR). Polymerase chain reaction was used to amplify the gene encoding the capsid protein from the DNA of PCV2. The protein was then cloned into a pRSET prokaryotic expression vector. Western blot analysis revealed that the recombinant protein gave strong signals on a polyvinylidene difluoride membrane when exposed to the serum from a pig infected with PCV2. The expressed protein was purified and used as an antigen for the ELISA and SPR study. A protein chip based on SPR was developed, and the diagnostic potential of SPR was compared with that of ELISA with the use of 70 serum samples obtained from 6 pig farms. There was a strong positive correlation between the ELISA and SPR titers (r = 0.877, P < 0.01). Therefore, this recombinant capsid protein can be used as an antigen for serologic studies, and the SPR, a label-free method, appears to be a valuable and reproducible tool in the serodiagnosis of a PCV2 infection.

Animals↗

PubMed2005

Statistical correlation between pharyngitis and temporomandibular joint disease.

OBJECTIVE: The objective of this study was to evaluate the upper respiratory infection (URI) as an aggravating factor in the established temporomandibular joint (TMJ) disease. PATIENTS AND METHODS: Four hundred seventeen patients suffering from temporomandibular disorder (TMD) were selected and investigated by means of questionnaires and clinical examinations. After excluding the patients with only muscle disorders, 283 patients were included for the association study between TMJ disease and infectious conditions. The screened infectious conditions were otitis media, maxillary sinusitis/rhinitis, and pharyngitis/tonsillitis. The chi-square test was used to determine the association between variables and stepwise logistical regression was then used. RESULTS: The prevalence of maxillary sinusitis/rhinitis in TMD patients was 7.0%. The patients who had mouth-opening limitation were 9.93 times more likely to have maxillary sinusitis/rhinitis than those without ( P = .0004). The prevalence of tonsillitis/pharyngitis in TMD patients was 9.1%. The patients who had mouth-opening limitation were 3.50 times more likely to have tonsillitis/pharyngitis than those without it ( P = .0028). The patients who had TMJ capsulitis were 3.91 times more likely to have tonsillitis/pharyngitis than those without it ( P = .0028). CONCLUSION: The conclusion is made that pharyngitis/sinusitis is significantly associated with some clinical symptoms of TMD. The infection of closely related anatomical structures with TMJ may have an influence on TMJ symptoms. Thus, pharyngitis/sinusitis in the established TMD patients can be a significant warning sign for TMJ symptoms to appear shortly thereafter.

Adult↗

PubMed2004

A multi-layered application for the gross description using Semantic Web technology.

OBJECTIVE: Development of a Semantic Web technology based system for the formalization of the gross description. METHOD: A system is developed using the Java-2 platform. It is based on a light-weight version of the Galen top level ontology. Web technologies like XML, SAX en DOM have been used. RESULT: Three system components have been developed to support the semantic, the object and the syntax layers of the PathOnt architecture. CONCLUSION: The PathOnt approach provides a tool for the communication among clinicians and technicians involved in pathology examinations. This tool also provides a foundation for linking the specimen-specific data with the controlled medical ontology so that the stored information can be used in different circumstances.

Internet↗

PubMed2004

Mutational hot spot in the DSPP gene causing dentinogenesis imperfecta type II.

The current system for the classification of hereditary defects of tooth dentin is based upon clinical and radiographic findings and consists of two types of dentin dysplasia (DD) and three types of dentinogenesis imperfecta (DGI). However, whether DGI type III should be considered a distinct phenotype or a variation of DGI type II is debatable. In the 30 years since the classification system was first proposed, significant advances have been made regarding the genetic etiologies of inherited dentin defects. DGI type II is recognized as an autosomal dominant disorder with almost complete penetrance and a low frequency of de novo mutations. We have identified a mutation (c.52G-->T, p.V18F) at the first nucleotide of exon 3 of the DSPP (dentin sialophosphoprotein) gene in a Korean family (de novo) and a Caucasian family. This mutation has previously been reported as causing DGI type II in a Chinese family. These findings suggest that this mutation site represents a mutational "hot spot" in the DSPP gene. The clinical and radiographic features of these two families include the classic phenotypes associated with both DGI type II and type III. Finding that a single mutation causes both phenotypic patterns strongly supports the conclusion that DGI type II and DGI type III are not separate diseases but rather the phenotypic variation of a single disease. We propose a modification of the current classification system such that the designation "hereditary opalescent dentin" or "DGI type II" should be used to describe both the DGI type II and type III phenotypes.

Child, Preschool↗

PubMed2002

Expression of membrane type I-matrix metalloproteinase in oral squamous cell carcinoma.

A local invasion and lymph node metastasis (LNM) of an oral squamous cell carcinoma (OSCC) has a poor prognosis, and involves the degradation of the extracellular matrix mediated by multiple proteolytic enzymes including membrane type I-matrix metalloproteinase (MT1-MMP). This study aimed to determine the role of MT1-MMP in OSCC, to evaluate the immunohistochemical expression of MT1-MMP with regard to the invasiveness and LNM of the OSCC, and to evaluate the major source of MT1-MMP mRNA and its protein using immunohistochemistry and in situ hybridization. MT1-MMP expression was examined in 46 OSCCs via immunohistochemistry and non-radioisotope in situ hybridization. The relationship between MT1-MMP expression and LNM, as well as the histological invasiveness, was statistically analyzed. The results showed that whereas 12 out of the 18 OSCCs (66.7%) with LNM showed moderate to strong MT1-MMP expression, only nine of the 28 OSCCs (32.1%) without LNM expressed MT1-MMP strongly. MT1-MMP expression was significantly higher with regard to LNM (P=0.022). As the invasion grade became stronger (from grade a to grade d), MT1-MMP was significantly more strongly expressed (P=0.033). These results suggest that MT1-MMP is primarily secreted in the OSCC cells and is involved in the invasiveness of the OSCC and LNM. Moreover, MT1-MMP combined with other markers may be used to predict the metastatic potential of an OSCC.

Adult↗

PubMed2002

Tissue levels of matrix metalloproteinases in pulps and periapical lesions.

The purpose of this study was to evaluate the tissue levels of matrix metalloproteinase (MMP)-1, -2, -3 and their distributions in inflamed human dental pulps and periapical lesions. Samples were subjected to enzyme-linked immunosorbent assay and/or immunohistochemistry by using specific antibodies to MMP-1, -2, and -3. Results from enzyme-linked immunosorbent assay were analyzed by using the Mann-Whitney U test and presented as p values. The concentrations of MMP-1 in all experimental groups were significantly higher than in the control (p < 0.05). The acute pulpitis and control groups were significant different in terms of their MMP-2 levels (p < 0.05). The concentration of MMP-3 in acute pulpitis was significantly higher than the control and chronic pulpitis groups (p < 0.05). Immunohistochemically, MMP-1 and MMP-3 were localized in the infiltrating neutrophils, macrophages, and extracellular matrix of the acute pulpitis group. These results suggest that MMPs play an important role in the pulp tissue destruction of acute, inflamed pulp.

Acute Disease↗

PubMed2002

Characterization of novel cell lines established from three human oral squamous cell carcinomas.

Human oral squamous cell carcinoma cell lines (KOSCC-11, -25A, -25B, -25C, -25D, -25E, -33A, and -33B) were established by explantation culture from these oral squamous cell carcinomas. The histopathology of the primary tumors, in vitro growth characteristics, epithelial origin, in vitro anchorage-independency, in vivo tumorigenicity, the frequency of human papillomavirus (HPV) infections, and the status of proto-oncogenes, tumor suppressor genes, DNA mismatch repair genes, and microsatellite instability were investigated in the cell lines. KOSCC-11 is a well-differentiated oral squamous cell carcinoma (OSCC) derived from mandibular gingiva. KOSCC-25A, -25B, -25C, -25D, and -25E cell lines were derived from the same OSCC. KOSCC-33A and -33B were established from the same tumor that originated from the maxillary sinus. All tumor lines studied grew as monolayers and showed: i) epithelial origin by the presence of desmosome and keratin; ii) in vitro anchorage-independent growth ability; and iii) tumorigenic potential in nude mice. The cancer cell lines did not contain HPV DNA and did not express viral genes. Northern blot analysis revealed: i) overexpression of EGFR in four cell lines, ii) overexpression of c-H-ras in four cell lines, iii) overexpression of c-myc in three cell lines, iv) decreased expression of TGF-alpha in seven cell lines, and v) decreased expression of c-jun in five cancer cell lines compared with normal human oral keratinocytes. In all KOSCC cell lines and their corresponding tumor tissues, mutations were identified in highly-conserved functional regions of the p53 gene. The KOSCC-11 cell line contained a frameshift mutation and the other cell lines harbored an identical p53 mutation at codon 175 from CGC (Arg) to CTC (Leu). In five cell lines, a significant reduction of p21WAF1/Cip1 protein was evident. Cancer cell lines expressed higher level of Rb protein than normal human oral keratinocytes. DCC, a tumor suppressor gene, was not detected in KOSCC-25C. The KOSCC-33A cell line displayed microsatellite instability and showed a loss of hMSH2 expression. These well-characterized human OSCC cell lines should serve as useful tools for understanding the biological characteristics of oral cancer.

Adaptor Proteins, Signal Transducing↗