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Biomedical subjects

James J Greene

Publications and source records attributed to James J Greene.

2 recordsLinked to original sources

Redox-mediated bypass of restriction point via skipping of G1pm.

BACKGROUND: It is well known that cancer cells bypass the restriction point, R, and undergo uncontrolled cell proliferation. HYPOTHESIS AND EVIDENCE: We suggest here that fibrosarcoma cells enter G1ps directly from M, skipping G1pm, hence bypassing R, in response to redox modulation. Evidence is presented from the published literature that demonstrate a shortening of the cycle period of transformed fibroblasts (SV-3T3) compared to the nontransformed 3T3 fibroblasts, corresponding to the duration of G1pm in the 3T3 fibroblasts. Evidence is also presented that demonstrate that redox modulation can induce the CUA-4 fibroblasts to bypass R, resulting in a cycle period closely corresponding to the cycle period of fibrosarcoma cells (HT1080). CONCLUSION: The evidence supports our hypothesis that a low internal redox potential can cause fibrosarcoma cells to skip the G1pm phase of the cell cycle.

Bone Neoplasms↗

Host cell compatibility in protein expression.

The expression of cloned genes in prokaryotic or eukaryotic host cells provides the means not only for the study of gene function but also for the production of substantial amounts of protein and nonprotein molecules for commercial and investigational use. In the case of proteins, strategies for determining the most appropriate vector-host combination for the expression of an exogenous gene depend on a diverse range of factors that relate ultimately to the properties of the gene and its product. The approach used in the downstream purification of the product is another factor that impinges on this selection. However, among the most important considerations in the choice of vector and host in ensuring the maximal amount of expression is the compatibility of the host cells to translate the RNA transcript, to ensure the proper folding of the product, and to sustain the protein in the intact and functional state.

Bacteria↗