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Biomedical subjects

Jan Kotarski

Publications and source records attributed to Jan Kotarski.

At least 19 recordsLinked to original sources
PubMed2005

Leptin is not involved in the pathophysiology of endometriosis-related infertility.

OBJECTIVE: Changes in peritoneal fluid (PF) composition may affect fertilization as well as early embryonic development. Leptin, an adipocyte hormone, has been shown to act as a link between adipose tissue and the reproductive system. Therefore, we decided to assess peritoneal and serum leptin levels in infertile endometriotic patients. PATIENTS: Seventy-two women were studied, including 30 fertile and 18 infertile women with ovarian endometriotic cysts and, as a reference group, 24 patients with unexplained infertility. RESULTS: No significant difference in the peritoneal and leptin levels was found between the studied groups. Significantly higher PF leptin concentration was observed in patients with stages III and IV of endometriosis as compared to those with minimal stage of the disease. In fertile patients with endometriosis a positive correlation has been found between PF and serum leptin concentrations. CONCLUSIONS: No differences in peritoneal or serum leptin levels between infertile and fertile women with endometriosis suggest that this cytokine is not involved in pathophysiology of endometriosis-related infertility.

Adolescent↗
PubMed2005

Serum acute phase protein concentrations after hysterectomy with and without low-molecular-weight heparin thrombosis prophylaxis.

Background. Low-molecular-weight heparin (LMWH) is recommended for venous thrombosis prophylaxis. There are several lines of evidence that heparins exhibit anti-inflammatory properties in addition to their anticoagulant activity. Therefore, we decided to estimate the concentrations of C-reactive protein (CRP) and haptoglobin, as acute-phase proteins, in the blood serum of patients who underwent total abdominal hysterectomy with and without the LMWH administration. Method. Forty-two women were studied, including 26 patients treated with enoxaparin and a group of 16 women without thrombosis prophylaxis. Haptoglobin and CRP concentrations were measured in serum samples obtained before the operation and also 8, 24, 48, 72, 96, and 192 hr after surgery. Results. Serum CRP level measured 8 hr after the operation was significantly higher in the group without thrombosis prophylaxis, however, no statistically significant differences in the concentration of CRP between studied groups in the other time periods were found. There were no statistically significant differences in serum haptoglobin levels between the groups of patients in all time periods. Conclusion. Administration of low-molecular-weight heparin may affect CRP production, however, further studies are still needed in order to clarify the exact immunosuppressive action of heparins.

Acute-Phase Proteins↗
PubMed2004

Serum cortisol and acute phase protein concentrations after hysterectomy with and without peritoneal sutures.

OBJECTIVE: Although non-closure of the peritoneum still remains a controversial problem, to our knowledge there have been no studies comparing stress response in women who either have or have not had peritoneal sutures. Therefore, we decided to assess cortisol, C-reactive protein and haptoglobin levels in serum obtained following hysterectomy in both sets of patients. MATERIAL AND METHODS: Forty-nine women after total abdominal hysterectomy with bilateral salpingo-oophorectomy were studied, including 28 patients with non-closed peritoneum and 21 women after peritoneal sutures. Cortisol and acute phase protein levels were measured in serum samples obtained before the operation and also 8, 24, 48, 72, 96 and 192 h after surgery. RESULTS: There were no significant differences in the concentration of cortisol and C-reactive protein in blood serum between groups of patients in all time periods. Serum haptoglobin level was not statistically significant between the examined groups in 8, 24, 48, 72, 96 h, but after 192 h it increased significantly in a group of patients who had received peritoneal closure operations. CONCLUSIONS: Our results suggest that closure of parietal and visceral peritoneum does not provide any benefits during the postoperative period.

Acute-Phase Proteins↗
PubMed2004

The effect of oscillating low intensity magnetic field on the Na+, K+, Ca++, and Mg++ concentrations in the maternal and fetal circulation of the dually perfused human placental cotyledon.

Dual-sided perfusions of the human placental cotyledon in vitro were used to study effects of low intensity magnetic fields (MFs) of 2 mT, 50 Hz (E1, 10 perfusions) and 5 mT, 50 Hz (E2, 10 perfusions). In the control group C (10 experiments) no field was used. Perfusions lasted 180 min each. Increased release of calcium ions from the placental cotyledon was found in the fetal circulation during perfusion when the 2 mT, 50 Hz MF was used. No changes in the release of sodium and magnesium ions were observed compared to the control group. The 5 mT, 50 Hz oscillating MF intensified the release of sodium ions from the perfused cotyledon both to the fetal and maternal circulation up to the 150th min of the experiment. Increased release of magnesium ions was observed only to the fetal circulation between 120 and 180 min and of calcium ions to the fetal circulation between 60 and 180 min. No significant differences in K concentrations were found between the control and MF exposed cotyledons under conditions of these experiments.

Calcium↗
PubMed2004

[Oxygen transfer and consumption in human placenta exposed to variable magnetic fields in vitro].

The initial investigations concerning the influence of variable magnetic fields (MF) on transfer and oxygen consumption in isolated human cotyledon in vitro were performed. Ten dual closed perfusion of the human cotyledon were conducted in each group. The control group was not exposed to magnetic fields. In studied groups cotyledons were exposed to magnetic field: in the group B1 (B = 2 mT, f = 50 Hz), and in the group B2 (B = 5 mT, f = 50 Hz) for 180 min. Obtained results may suggest that variable magnetic field (B = 5 mT, f = 50 Hz) cause decrease of oxygen consumption in human placenta in 120 and 150 min of experiment.

Active Transport, Cell Nucleus↗
PubMed2004

[Decrease of paraoxonase activity in the first day after abdominal operations in women].

INTRODUCTION: Paraoxonase (PON1) is one of enzymes participating in antioxidative defence mechanism, which protects the organism from the results of action of reactive oxygen species. PON1 is an esterase contained in plasma high density lipoproteins (HDL) which protects HDL from peroxidation and plasma membranes from free radical injury. PON1 serum activity is diminished in some diseases (atherosclerosis, diabetes, coronary artery disease) and after cardiosurgery operations. But there is no information about changes in PON1 activity after abdominal operations. THE AIM: Of this study was the assessment of PON1 serum activity after the abdominal operations in women. MATERIALS AND METHODS: 40 women operated in the 1st Department of Gynaecology of Lublin Medical University were studied, including 34 patients after total abdominal hysterectomy with salpingo-oophorectomy and 6 patients after myomectomy or cyst enucleation. The blood to experiments was collected before operation, and 8 and 24 hours after. PON1 serum activity was assessed spectrophotometrically toward two synthetic substrates: paraoxon and phenyl acetate. RESULTS: PON1 activity toward paraoxon before operation was 146.8 +/- 17.3 U/ml, and after 8 and 24 hours after 107.6 +/- 11.9 U/ml and 119.9 +/- 16.9 U/ml, respectively (results statistically significant). PON1 activity toward phenyl acetate (arylesterase activity) before operation was 182.7 +/- 16.4 U/ml and did not significantly changed after the surgery. CONCLUSION: It is observed decreased PON1 serum activity in the early postoperative period after abdominal operations in women, what can be connected with the utilisation of PON1 in antioxidative defence mechanism.

Adult↗
PubMed2004

[Pre-pubic TVT in the treatment of urinary incontinence in women. Description of surgical technique and initial results].

Tension-free vaginal tape (TVT) has within the last decade become one of the most popular procedure for treatment of female urinary stress incontinence. It is still persistent the risk of complications peri- and postoperative in spite of high efficacy of this method. Investigators still have been looking for new variations of this method to diminish the risk of complications and to maintain the efficacy of the treatment. The aim of this study is the description of the pre pubic TVT method and the preliminary short time (3 month) results of treatment. The results of pre-pubic TVT treatment were compared with "classic" TVT method. The pre-pubic TVT technique was performed in 25 women, operated for stress incontinence. The average operation time was 19 min (10-30 min). During the operation in one of patients there was excessive bleeding from venous plexes of vagina during preparation of tissues and in 4 of them it was perforation of vaginal mucosa during introducing of needle with tape. All these complications were recognised and corrected during the surgery. The mean hospitalisation time was two days. Our 3-months observations allow to confirm that efficacy of pre-pubic TVT is similar to the "classic" method, but the frequency of complications is lower in the pre-pubic TVT. There were no postoperative complications and voiding dysfunctions.

Adult↗
PubMed2003

Investigation of glutathione concentrations in peritoneal fluid from women with and without endometriosis.

UNLABELLED: Changes in the peritoneal fluid (PF) environment have been implicated in the pathogenesis of endometriosis as well as in the decrease of fertility. OBJECTIVE: To evaluate the concentration of glutathione in PF of women with endometriosis. PATIENTS: Twenty-one patients with endometriosis (I or II rAFS stage, n=11; III or IV rAFS stage, n=10), and 29 patients with follicular or dermoid ovarian cysts (n=17 and n=12, respectively). RESULTS: Mean (+/-S.D.) PF glutathione concentration was 0.22+/-0.01 micromol/ml in patients with minimal or mild endometriosis, 0.21+/-0.05 micromol/ml in women with III or IV stage of the disease, 0.24 +/- 0.03 micromol/ml in women with follicle ovarian cysts, and 0.23+/-0.05 micromol/ml in patients with dermoid tumors of ovaries. No significant difference in the peritoneal glutathione level was found between the groups. CONCLUSION: These results suggest that PF glutathione is not involved in the progression of endometriosis.

Adult↗
PubMed2003

Evaluation of myeloid and lymphoid dendritic cells in peritoneal fluid in women with non-malignant ovarian tumors.

PROBLEM: Identification of myeloid and lymphoid dendritic cells (DCs) in peritoneal fluid (PF) and peripheral blood (PB) of patients with ovarian pathology. METHOD OF STUDY: PF and PB were collected from 60 patients who underwent laparoscopy because of non-malignant ovarian tumors. Mononuclear cells were separated by gradient centrifugation. The cell surface antigens were determined by flow cytometry using monoclonal antibodies. RESULTS: Both myeloid and lymphoid DCs were detected in PF and PB of women with ovarian tumors. The percentage of myeloid DCs was significantly higher in PF than in PB. The concentration of PF myeloid DCs was the highest (P < 0.05) in patients with dermoid cysts (0.67 x 10(6)/mL PF) in comparison with the other studied groups, excluding patients with normal pelvis. CONCLUSIONS: Domination of myeloid and not lymphoid cells in PF may support the hypothesis that local PF immune disturbances may play a role in some non-malignant ovarian pathology.

Adolescent↗
PubMed2003

[Identification of myeloid dendritic cells in peritoneal fluid by flow cytometry].

The myeloid lineage-derived DCs are the most potent antigen-presenting cells (APC), which are highly specialized in capturing antigens and triggering adaptive immune responses. The purpose of our study was to identify of the myeloid DCs in peritoneal fluid of women with nonmalignant ovarian tumors. With use of flow cytometry we detected myeloid dendritic cells in peritoneal fluid of the studied patients. Myeloid DCs were identified as a discrete population of mononuclear cells expressing high levels of HLA-DR and CD33, but without expression of CD14 and CD16. The number of DCs was shown as a percentage of mononuclear cells. Dendritic cells comprised 3.02% (0.35%-8.41%) in patients with ovarian tumors and 1.72% (0.51%-6.2%) of peritoneal fluid mononuclear cells in women with unexplained infertility. The percentage of DCs in peritoneal fluid was significantly higher than in peripheral blood.

Adult↗
PubMed2003

[Uterine arteries embolization as a treatment of uterine leiomyoma].

Uterine artery embolization is a new method of treating uterine leiomyomata, first carried out in France in the early 90s. The procedures involve placing a small catheter into an artery in the groin and directing it to the blood supply of the fibroid. Little plugs of polyvinyl alcohol are injected through the catheter to block these arteries. This cause the fibroid to shrink. Indications for uterine fibroid embolization include menorrhagia, pelvic pain or pressure, other "bulk" syndrome (low-back pain, urinary frequency and constipation. The fluoroscopic-guided procedure is performed under local anesthesia. Most patients are discharged within 72 hours. Post-embolization syndrome including severe pain is managed with morphine via patient-controlled pump. Paper reviews long term outcomes. Uterine artery embolization has several advantages: high efficacy, less invasiveness, ability to treat multifocal changes, uterine preservation, shorter hospitalisation and recovery (low cost) and disadvantages: postembolic syndrome (pain and fever), unknown relations to pregnancy and lack of long term results.

Chemoembolization, Therapeutic↗
PubMed2003

Successful treatment with recombinant factor VIIa for intractable bleeding at pelvic surgery.

BACKGROUND: Postoperative bleeding may be complicated by failure to identify the site of bleeding, multiple bleeding points, and persistent hemorrhage despite surgical intervention. CASE: Activated recombinant factor VII was used successfully in a patient with life-threatening postsurgical bleeding after resection of two large extraperitoneal pelvic sarcomas. CONCLUSION: In severe hemorrhage, hemostasis can be achieved with activated recombinant factor VII.

Factor VIIa↗
PubMed2003

[Non-closure of the peritoneum after hysterectomy--postoperative clinical assessment].

Dynamic development in modern medicine permanently brings the modifications of operation techniques. Both closure and non-closure of the peritoneum after gynecologic operations has been discussed recently. According to clinical observations provided by many authors, the controversial problem of non-closure of the peritoneum is still under discussion. Thus we decided to assess the influence of closing or non-closing of the peritoneum on short-term postoperative morbidity. The patients operated because of uterine myomae were the participants of our study. Total abdominal hysterectomy with suturing or non-closing of visceral and parietal peritoneum was performed. Our study revealed that non-closing of the peritoneum did not increase the amount of postoperative complications and it seemed to be a safe procedure.

Adult↗
PubMed2003

[Application of unabsorbable polypropylene mesh in patients with vaginal prolapse after hysterectomy].

OBJECTIVES: Vaginal prolapse is one of delayed complications of hysterectomy. DESIGN: The aim of this work is estimation of efficiency of the application of unabsorbable polypropylene mesh in patients with vaginal prolapse after a prior operation of hysterectomy. MATERIALS AND METHODS: In the work the authors have described the operation procedure. It was performed in 6 women aged 49-73. Patients were inspected in 1, 3 and 6 months after the operation. RESULTS: The authors have estimated anatomical relations and postsurgical complications. CONCLUSION: The operation with the use of unabsorbable polypropylene mesh is an easy and efficient method of treatment of vaginal prolapse in patients after hysterectomy.

Aged↗
PubMed2003

[Evaluation of the morphology of the human placental cotyledon following dual in vitro perfusion in variable magnetic field].

UNLABELLED: Objectives and the aim of the study was electron-microscopy morphological estimation of the human placental cotyledon after 180 minutes of dual closed perfusion in vitro. MATERIALS AND METHODS: In the experimental group the cotyledons were exposed to variable magnetic field of 2 mT magnetic induction and 50 Hz frequency. The control group K (10 perfusions) was not subjected to magnetic field while the experimental group B (10 perfusions) was influenced by magnetic field. RESULTS: It was found that homogeneous variable magnetic field disturbs the ultrastructure of the nuclei and cytoplasma and it increases the density of the vascular-epithelial membrane of villi cells of human placenta in vitro. CONCLUSION: Variable sinusoildal, magnetic field of 2 mT magnetic induction and 50 Hz frequency disturbs the ultrastructure of the nuclei and cytoplasma and it increases the density of the vascular-epithelial membrane of villi cells of human placenta in vitro after 180 minutes of dual closed perfusion in vitro.

Cell Nucleus↗
PubMed2003

[Assessment of serum lipid peroxide levels and antioxidant status in females who had undergone total abdominal hysterectomy without closing of the peritoneum].

INTRODUCTION: Reactive oxygen species (ROS) generated after the trauma caused by surgical intervention, have the capacity to react in an indiscriminate manner leading to damage to almost any cellular component (proteins, nucleid acids) and to lipid peroxidation. The assessment of ROS generation intensity process may help in understanding of molecular processes observed in the intracellular environment after operations. The evaluation of antioxidative level may give an answer about the efficiency of antioxidative defence mechanism. The peritoneum is an organ of high metabolic activity, connected with ROS generation and participated in healing process. The modern opinions suggest closing of postoperative wound without closing of peritoneum. The aim of this study was assessment of antioxidant status and serum lipid peroxide levels in early postoperative period, in females who had undergone total abdominal hysterectomy without closing of peritoneum. MATERIALS AND METHODS: 30 women after total abdominal hysterectomy with salpingooophorectomy were studied, including 13 patients after peritoneal suturing (control group--C) and 17 women with nonclosed peritoneum (study group--S). The total serum antioxidant status (FRAP) was evaluated spectrophotometrically. The concentration of lipid peroxidation products was assessed in serum collected before operation and 8 and 24 hours after, as the concentration of lipid hydroxyperoxides (HPETE), malonyl dialdehyde (MDA) and 4-hydroxyalkenals (4-HDA). RESULTS: The antioxidative level before operation was 1147.09 +/- 93.6 microM/l in S group; 1022.04 +/- 115.4 microM/l in C group and did not changed significantly in early postoperative period. MDA + 4-HDA level before operation was 1.08 +/- 0.2 microM/l in S group and 1.06 +/- 0.2 microM/l in C group; HPETE level before operation was 4.58 +/- 0.1 microM/l in S group and 4.72 +/- 0.1 microM/l in C group, 8 and 24 hours after the operation MDA + 4-HDA level increased respectively to 1.34 +/- 0.2 microM/l (by 27%; p > 0.05) and 1.46 +/- 0.2 microM/l (37%) in S group and to 1.16 +/- 0.2 microM/l (by 8%) and 1.62 +/- 0.2 microM/l (by 52%; p < 0.05) in C group. HPETE level decreased to 4.56 +/- 0.1 microM/l (by 1%) and increased to 5.23 +/- 0.2 microM/l (by 14%) in S group and increased respectively to 6.0 +/- 0.2 microM/l (by 27%; p < 0.01) and to 6.43 +/- 0.2 microM/l (by 36%; p < 0.01) in C group. CONCLUSIONS: The concentration of lipid peroxidation products after the operation was lower in study group, where peritoneum was left without closing. Total antioxidative level was the same in both groups. Obtained results suggest smaller ROS generation in study group.

Biomarkers↗
PubMed2003

[Color Doppler blood flow measurements and microvessel density assessment in ovarian tumors].

OBJECTIVE: The aim of our study was to compare ultrasound vascular blood flow indices with the expression of CD-34 in women with benign and malignant adnexal tumors. MATERIALS AND METHODS: Transvaginal color and pulsed Doppler sonography was performed before surgical procedure in 105 women with adnexal tumors. Blood flow indices: pulsatility index (PI) and resistive index (RI) in blood vessels with the highest flow velocity were measured within each tumor. Immunohistochemical analysis of microvessel density (MVD) was performed on representative highly vascular tumor specimens fixed in 10% formalin and paraffin embedded. The primary monoclonal mouse anti-human CD-34 antibody (1:25, DAKO, Denmark) was used. Statistical calculations included group comparison with Kruskal-Wallis ANOVA on ranks and correlation analysis (Statistica for Windows 6.0, Statsoft Poland). RESULTS: Mean age of the studied women was 55.6 +/- 11.5 yrs. Of 104 tumors, 35 (33.6%) were benign and 69 (66.4%) were malignant. The latter included 14 FIGO stage I cases. Low resistance or pulsatility indices (RI < 0.5 or PI < 0.8) were found in 58 of 69 (84%) malignant masses and in 21 of 35 (60%) benign tumors. Substantial overlap in measured indices was found between both studied groups. Median number of microvessels per high power field in the benign and malignant tumors were 37 (range: 14-156) and 68 (range: 25-177), respectively. These differences were statistically significant (p = 0.007, Mann-Whitney U test). Also, a significant correlation was found between MVD and low resistance to blood flow as measured by the PI or RI (r = 0.43, p < 0.05). However, no statistically significant differences in MVD were found between FIGO stage I and other stages of ovarian cancer. CONCLUSION: Low resistance to blood flow as measured by the resistive or pulsatlity indices used in color Doppler sonography may by positively correlated with the microvessel density in the malignant ovarian tumors.

Adult↗