Biomedical subjects

Jan Sulc

Publications and source records attributed to Jan Sulc.

5 recordsLinked to original sources

PubMed2006

Cytokine gene polymorphisms and high-resolution-computed tomography score in idiopathic pulmonary fibrosis.

INTRODUCTION: Idiopathic pulmonary fibrosis (IPF) is a serious disease with unknown cause and the influence of cytokine gene polymorphisms is presumed in the etiology and pathogenesis of the disease. We used high-resolution computed tomography (HRCT) as a marker of disease stage and progression and compared the alveolar and interstitial score with IL-1, IL-4, IL-12, IL-1RA and IL-4RA cytokine gene polymorphisms. SUBJECTS AND METHODS: The IPF patients were all Caucasians from the Czech Republic and consisted of 20 females and 10 males, with a mean age of 65 years, range 36-85. The HRCT results were evaluated by an experienced viewer using the interstitial and alveolar score scales, which were based on the IPF HRCT description system from Gay SE, Kazerooni EA, Tows GB, Lynch JP, Gross BH, Cascade PN, et al. [Idiopathic pulmonary fibrosis. Predicting response to therapy and survival. Am J Respir Crit Care Med 1998;157:1063-72]. We evaluated the polymorphisms of cytokine genes utilizing a PCR with sequence-specific primers method. RESULTS: The HRCT alveolar score was more pronounced in IL-4 RA (+1902) AG heterozygotes. The HRCT interstitial score was less severe in the IL-12 (-1188) AA homozygotes. According to progression of the HRCT interstitial score, the CC homozygosity at IL-1 RA (mspa 111100), the AA homozygosity at IL-4 RA (+1902) and CC homozygosity at IL-4(+33) positions were more frequent in patients with stable disease compared to those with progressive disease. CONCLUSIONS: We assume from our data that the polymorphisms of IL-4, IL-4RA, IL-1RA and IL-12 genes (genes of cytokines with regulatory activity) might influence the phenotype of IPF as shown by measurable changes in HRCT investigations.

Adult↗

PubMed2004

X-ray opaque waveguide for dentistry.

OBJECTIVE: The aim of this study is the design, realization, and examination of a new x-ray contrast hollow waveguide which will be suitable for x-ray diagnostics or treatment required in medicine, as dentistry, maxiollofacial surgery or oncology. BACKGROUND DATA: With the growing usefulness of laser radiation therapeutic interventions, a strong demand for radiation delivery from the laser source to the interaction place has appeared. For more complicated surgery, especially for internal intervention, an x-ray is necessary. In such cases, as a minimum, the end of the delivery system must be x-ray contrasted to distinguish where the interaction of radiation with tissue would take place. Up to now, neither fiber, hollow waveguide, nor other delivery system have been x-ray opaque. MATERIALS AND METHODS: A new type of hollow glass waveguide was designed, fabricated, and examined as a delivery system for an endodontic treatment. The system is composed from the cyclic olefin polymer coated silver hollow glass waveguide with special covering for x-ray visibility. The inner diameter of the waveguide was 320 microm, the outer diameter was 630 microm, and its length was 100 mm. After the delivery system was created, the hollow waveguide was checked under an x-ray machine and its opacity was measured. For actual treatment, an Er:YAG laser system generating a mid-infrared radiation was used. The root canals of 10 molars were treated endodontically, and the result of that treatment was compared with the common cleaning methods. During treatment, digital x-ray images were taken with conventional files, using this new designed hollow waveguide. RESULTS: The position of a metal instrument demonstrates the quality of opacity after conventional step-back technique. The hollow waveguide is visible in the left tooth canal. A bone density analysis shows no differences between a left (waveguide) and right (tooth) canal. CONCLUSION: It was demonstrated that no differences exist between x-ray opacity of the metal instrument used for endodontic treatment and a special hollow waveguide. The digitalized, three-dimensional image helps to detect a precise position of customary instrument or waveguide in root canal.

Equipment Design↗

PubMed2004

Diode laser-activated bleaching.

This study describes a preclinical investigation with laser-activated bleaching agent for discolored teeth. Bleaching techniques involve a broad-spectrum approach utilizing hydrogen peroxide (3-38%) with or without heat or laser, carbamide peroxide (10-30%), or a mixture of sodium perborate and hydrogen peroxide. Extracted human maxillary central incisors were selected. In the bleaching experiment, 38% hydrogen peroxide was used. Two different laser systems and light emission diodes for activation of the bleaching agent were used: diode laser, wavelength 970 nm, and infra-red diode laser, wavelength 790 nm, with eight blue light emission diodes, wavelength 467 nm. The enamel surface was evaluated with the scanning electron microscope. The method of chemical oxidation resulted in a 2-3 shade change in one treatment (15 min). Shorter time (5 min) was not effective. The diode laser, wavelength 970 nm, and the bleaching agent produced the same effect but with a shorter time of bleaching process (5 min - 1 W, 2.5 min - 2 W). Infra-red diode laser, wavelength 790 nm with eight blue light emission diodes, wavelength 467 nm, and the bleaching agent reached the desired color shade also after a shorter time (5 min - 40 mW). Slight surface modification after the bleaching process was detected with scanning electron microscopy. Currently, the laser has been proven the most valuable energy source for power bleaching with simple and short application in the dental office.

Hydrogen Peroxide↗

PubMed2003

Erbium:YAG laser lithotripsy by use of a flexible hollow waveguide with an end-scaling cap.

An Er:YAG laser light delivery system composed of a polymer-coated silver hollow waveguide and a quartz sealing cap has been developed for calculus fragmentation. Sealing caps with various distal-end geometries were fabricated, and the focusing effects of these caps for Er:YAG laser light were measured both in air and in water. Owing to the high power capability of the quartz a beam of sealing caps, Er:YAG laser light with an output energy of 200 mJ and a repetition rate of 10 Hz was successfully transmitted in saline solution by use of the system. Calculus fragmentation experiments conducted in vitro showed that the delivery system is suitable for medical applications in lithotripsy. We also found that the cap with a focusing effect is more effective in cutting calculi. The deterioration of the sealing caps after calculus fragmentation is also discussed.

Equipment Design↗

PubMed2002

Endodontic treatment with application of Er:YAG laser waveguide radiation disinfection.

OBJECTIVE: The objective of this study is to examine the ability of Er:YAG laser radiation. Using a movable waveguide helps to obtain an antibacterial effect, not only in root canal walls but also in the surrounding tissues. BACKGROUND DATA: Conventional endodontic treatment is not fully effective due to microbial colonization of root canal walls dentin in premolars and molars. Various laser systems seem to be effective to kill the remaining microbial content in the root canal. The problem is in the flexibility of laser system tips. MATERIALS AND METHODS: The Er:YAG laser system was designed with a fluorocarbon polymer-coated silver hollow glass waveguide. Root canal systems of 44 premolars and molars were treated endodontically, using a step-back technique; 10 teeth were then treated with calcium hydroxide paste, and 22 teeth were irradiated by a movable waveguide. Before and after treatment, the colony-forming units were counted to determine 21 various microorganisms. RESULTS: Classical enlargement and shaping of the root canal is effective in 60%. Application of calcium hydroxide prepares sterile root canal in 80%. Er:YAG laser irradiation via movable waveguide (energy of 100 mJ, 30 pulses, repetition rate 4 Hz) can ensure residual disinfection of the root canal. CONCLUSION: Application of Er:YAG laser radiation through a flexible waveguide helps to attain antibacterial effect, not only in the root canal walls, but also in the surrounding tissues. Therapeutic doses of laser radiation guarantee one-step disinfection, including of anaerobic microorganisms.

Dental Pulp Cavity↗