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Jane Mills

Publications and source records attributed to Jane Mills.

4 recordsLinked to original sources
PubMed2006

The malonyl transferase activity of type II polyketide synthase acyl carrier proteins.

Acyl carrier proteins (ACPs) play a fundamental role in directing intermediates among the enzyme active sites of fatty acid and polyketide synthases (PKSs). In this paper, we demonstrate that the Streptomyces coelicolor (S. coelicolor) actinorhodin (act) PKS ACP can catalyze transfer of malonate to type II S. coelicolor fatty acid synthase (FAS) and other PKS ACPs in vitro. The reciprocal transfer from S. coelicolor FAS ACP to a PKS ACP was not observed. Several mutations in both act ACP and S. coelicolor FAS ACP could be classified by their participation in either donation or acceptance of this malonyl group. These mutations indicated that self-malonylation and malonyl transfer could be completely decoupled, implying that they were separate processes and that a FAS ACP could be converted from a non-malonyl-transferring protein to one with malonyl transferase activity.

Acyl Carrier Protein↗
PubMed2006

Mentoring matters: developing rural nurses knowledge and skills.

Mentoring, the alliance of two people with varying degrees of experience in order to create opportunities for support and learning, is being encouraged as a response to current workforce issues in Australian rural nursing. The nursing profession is beginning to recognise the benefits of mentorship in providing psychosocial support and role acquisition skills to novice practitioners. These outcomes are valuable for enhancing the recruitment and retention of staff. The Mentor Development and Support Project of The Association for Australian Rural Nurses (AARN), established in 2003, was a strategy for increasing mentoring in the rural nursing workforce. This project aimed to develop the capacity of rural nurses to mentor effectively, as well as provide support for them for the duration of the mentoring partnership. This article reports on aspects of the AARN project. It includes a discussion of the need for planned mentor development opportunities and provides the rationale for the curriculum content of project workshops. It also includes evaluated outcomes for participants in the project.

Australia↗
PubMed2006

Adopting a constructivist approach to grounded theory: implications for research design.

Grounded theory is a popular research methodology that is evolving to account for a range of ontological and epistemological underpinnings. Constructivist grounded theory has its foundations in relativism and an appreciation of the multiple truths and realities of subjectivism. Undertaking a constructivist enquiry requires the adoption of a position of mutuality between researcher and participant in the research process, which necessitates a rethinking of the grounded theorist's traditional role of objective observer. Key issues for constructivist grounded theorists to consider in designing their research studies are discussed in relation to developing a partnership with participants that enables a mutual construction of meaning during interviews and a meaningful reconstruction of their stories into a grounded theory model.

Abstracting and Indexing↗