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Biomedical subjects

Jean Claude Franquin

Publications and source records attributed to Jean Claude Franquin.

2 recordsLinked to original sources

Dentin permeability and eugenol diffusion after full crown preparation.

PURPOSE: To compare teeth prepared to receive a metallic versus a metal-ceramic crown with respect to (1) in vitro dentin permeability before and after using a desensitizing agent and (2) pulpal eugenol concentration after sealing a temporary crown with a zinc oxide-eugenol based cement. MATERIALS AND METHODS: The roots of 20 human mandibular molars were separated and the crowns were prepared to receive a metallic crown. The hydraulic conductance was recorded before and after using Protect dentin desensitizer. The crowns were then further reduced to receive a metal-ceramic crown and the hydraulic conductance was recorded under the same conditions, on deeper dentin, before and after using the desensitizing agent. Twenty additional teeth were prepared: 10 to receive a metallic crown and 10 teeth to receive a metal-ceramic crown. A tube filled with 1 mL phosphate buffered saline (PBS) was sealed to the cementoenamel junction. A temporary crown was sealed onto the preparation with Temp Bond. The amount of eugenol that diffused across dentin into PBS was spectrofluorimetrically measured at Day 1 and Day 7. The crown preparations were vertically sectioned and the dentin remaining thickness was recorded. RESULTS: The hydraulic conductance of teeth prepared for a metal-ceramic crown was twice as high as teeth prepared for a metallic crown (P < 0.001). The desensitizing agent reduced the hydraulic conductance in both groups (P< 0.001). The two groups showed the same hydraulic conductance after using Protect (ns). No significant difference was found in the amount of eugenol diffusion between the two groups of teeth although eugenol diffusion decreased with time (P < 0.01). No correlation was found between eugenol diffusion and remaining dentin thickness.

Analysis of Variance↗

Efficiency and cytotoxicity of resin-based desensitizing agents.

PURPOSE: To compare in vitro the efficacy of five resin-based desensitizing agents at reducing human dentin permeability and to compare their cytotoxicity. The tested hypothesis was that their different curing techniques cause variations in efficiency and cytotoxicity. MATERIALS AND METHODS: Dentin slices (0.5 +/- 0.05 mm thick) were prepared from human third molars (10 per group) and their hydraulic conductance was recorded before and after application of one of the desensitizing agents with a Flodec device. Six desensitizing agents were studied: one light curing agent (Seal and Protect); one self-curing agent (Pain Free); the resin-based agents without any polymerization initiator (Health-Dent, Gluma Desensitizer, Isodan); one oxalate-based agent served as a control (Protect). A MTT assay on L 929 fibroblasts was performed to measure the cytotoxicity of the six desensitizing agents applied onto additional dentin slices (10 per group). RESULTS: All the desensitizing agents resulted in a large decrease in dentin permeability. The best results were obtained with Gluma Desensitizer, Isodan, Pain Free and Protect. A statistically significant difference was found among the materials (P = 0.001). All the materials were non-cytotoxic. Cell viability ranged from 88% for Seal and Protect to 100% for Isodan. No difference was found among their cytotoxicity.

Analysis of Variance↗