Biomedical subjects

Jean-Claude Jouanin

Publications and source records attributed to Jean-Claude Jouanin.

7 recordsLinked to original sources

PubMed2006

Modafinil-induced modulation of working memory and plasma corticosterone in chronically-stressed mice.

The original aims of our study were to investigate the dose-effect relationship of modafinil administration on working memory performance, in parallel with the measurement of plasma corticosterone in chronically-stressed mice, as compared to control mice. Memory performance was evaluated by spontaneous alternation in a T-maze. Vehicle or modafinil (8, 16 or 32 mg/kg) were administered after or without chronic stress (immobilization and exposure to light) for 15 min/day over a period of consecutive 14 days. Immediately after behavioral testing, blood was sampled to measure plasma corticosterone levels. Under non-stress conditions, corticosterone significantly increased with 16 and 32 mg/kg modafinil administration. Interestingly, optimal working memory performance was revealed at the 16 mg/kg dose. Moreover, no correlation was evidenced between working memory performance and plasma corticosterone level in modafinil-treated animals. Under stress conditions, corticosterone level was lowered at 8 mg/kg and remained unchanged at 16 and 32 mg/kg modafinil. An optimal working memory performance was evidenced at 8 mg/kg, which indicated a decrease in the efficiency threshold of modafinil under stress. Furthermore, an inverse correlation emerged between working memory performance and corticosterone level. Our study evidenced for the first time the interaction between stress and memory, in the emotional modulation of working memory performance, as a function of the administered dose of modafinil.

Animals↗

PubMed2005

Cerebral oxygenation declines despite maintained orthostatic tolerance after brief exposure to gravitational stress.

We examined the effect of a single 120 s of exposure to +3Gz (head-to-foot inertial forces) centrifugation as orthostatic stress on cerebral oxygenation (oxy-Hb) and cerebral blood volume (CBV) changes in response to stand test, in order to relate the occurrence of altered cerebral oxygenation control to any increase in sympathetic activity. Frontal near-infrared spectroscopy and mean arterial blood pressure at brain level (MAPbrain) were recorded in 14 subjects in supine and then in standing (10 min) position, before and after +3Gz centrifugation. The decrease in oxy-Hb (-7 +/- 5 a.u. versus -27 +/- 4 a.u., P<0.001) and in CBV (-6 +/- 10 a.u. versus -15 +/- 8 a.u., P<0.05) upon standing was more important after +3Gz centrifugation, with unchanged MAPbrain (-8 +/- 8 mmHg versus -3 +/- 11 mmHg). Upon standing, the high-frequency component of heart rate was lower (1090 +/- 460 ms2 versus 827 +/- 412 ms2, P<0.05) after +3Gz centrifugation. These findings suggest a downward shift in the static cerebral autoregulatory curve. We conclude that cerebral vasoconstriction might have occurred without centrally mediated increase in the entire peripheral sympathetic activity of the body.

Adult↗

PubMed2005

EEG and ECG changes during simulator operation reflect mental workload and vigilance.

BACKGROUND: Performing mission tasks in a simulator influences many neurophysiological measures. Quantitative assessments of electroencephalography (EEG) and electrocardiography (ECG) have made it possible to develop indicators of mental workload and to estimate relative physiological responses to cognitive requirements. OBJECTIVE: To evaluate the effects of mental workload without actual physical risk, we studied the cortical and cardiovascular changes that occurred during simulated flight. METHODS: There were 12 pilots (8 novices and 4 experts) who simulated a flight composed of 10 sequences that induced several different mental workload levels. EEG was recorded at 12 electrode sites during rest and flight sequences; ECG activity was also recorded. Subjective tests were used to evaluate anxiety and vigilance levels. RESULTS: Theta band activity was lower during the two simulated flight rest sequences than during visual and instrument flight sequences at central, parietal, and occipital sites (p < 0.05). On the other hand, rest sequences resulted in higher beta (at the C4 site; p < 0.05) and gamma (at the central, parietal, and occipital sites; p < 0.05) power than active segments. The mean heart rate (HR) was not significantly different during any simulated flight sequence, but HR was lower for expert subjects than for novices. The subjective tests revealed no significant anxiety and high values for vigilance levels before and during flight. CONCLUSIONS: The different flight sequences performed on the simulator resulted in electrophysiological changes that expressed variations in mental workload. These results corroborate those found during study of real flights, particularly during sequences requiring the heaviest mental workload.

Aerospace Medicine↗

PubMed2005

Aerobatic flight effects on baroreflex sensitivity and sympathovagal balance in experienced pilots.

BACKGROUND: Aerobatic flights subject pilots to accelerations and, therefore, to heavy physical workloads. OBJECTIVE: Our aim was to document changes in spontaneous baroreflex sensitivity and disturbances of sympathovagal balance after exposure to "push-pull" accelerations. METHODS: During 30-min flights, five aerobatic pilots performed five series of descending spirals: first, 30 s under negative (-3 Gz max), and then 30 s under positive (+4 Gz max) G loading, climbing between each series to regain altitude. A stand-test was performed before (T0), immediately postflight (PF), 1 h (PF1), and 2 h after (PF2) the flight. A Finapres apparatus recorded heart rate (HR) and BP during the stand-tests. RESULTS: Resting HR was higher at PF than T0 in supine (11.2 +/- 5.3%, p < 0.01) and standing (11.0 +/- 4.9%; p < 0.05) positions. Sequence analysis of spontaneous baroflex sensitivity (BRS) and spectral analysis of HR variability showed that: a) supine spontaneous BRS did not differ between preflight and postflight, while parasympathetic modulation of HR variability tended to increase; and b) supine spontaneous BRS was higher at PF1 than PF (PF: 0.011 +/- 0.0014 ms x mmHg(-1), PF1: 0.015 +/- 0.0012 ms x mmHg(-1); p < 0.05) and parasympathetic modulation of HR variability (high frequency component) was higher at PF2 than PF (PF: 0.014 +/- 0.007, PF2: 0.039 +/- 0.009; p < 0.001). CONCLUSIONS: These findings may reflect a change in the sympathovagal balance during the second hour of recovery from repeated push-pull maneuvers.

Acceleration↗

PubMed2004

Declarative memory impairments following a military combat course: parallel neuropsychological and biochemical investigations.

The aim of this study was to investigate the impact on several forms of memory and metabolism of a 5-day combat course including heavy and continuous physical activities and sleep deprivation. Mnemonic performance and biochemical parameters of 21 male soldiers were examined before and at the end of the course. Our results showed that short-term memory (memory span, visual memory, audiovisual association) and long-term memory were significantly impaired, whereas short-term spatial memory and planning tasks were spared. Parallel biochemical analysis showed an adaptation of energy metabolism. The observed decrease in glycaemia may be partly responsible for the long-term memory impairment, whereas the decreases in plasma cholinesterases and choline may be involved in the short-term memory deterioration. However, there are also many other reasons for the observed memory changes, one of them being chronic sleep deprivation.

Adult↗

PubMed2004

Analysis of heart rate variability after a ranger training course.

We studied the effects of prolonged physical activities on resting heart rate variability (HRV) during a training session attended by 23 cadets of the French military academy. This course lasts 1 month and is concluded by a 5-day field exercise simulation with physical and psychological stress. Data collection took place before (B) and immediately at the end (E) of the course. It included HRV recordings during a stand test (5 minutes lying down and 5 minutes standing), with a Polar R-R monitor, followed by blood sampling to assay plasma testosterone. The results (B and E) showed that the testosterone level fell by approximately 28.6 +/- 7%, indicating a high level of fatigue. During the stand test, the total power (TP) of the HRV spectrum increased in a supine position. The TP of B was 5,515.7 ms2 (SE, 718.4) and of E was 13018.9 ms2 (SE, 2,539.2; p < 0.001). High-frequency (HF) normalized values increased and low-frequency (LF) normalized values fell, regardless of position (HF normalized values and LF normalized values: supine, p < 0.01, p < 0.05; standing, p < 0.05, p < 0.01, respectively). LF:HF ratio fell 66.2 (SE, 12.9%; p < 0.01) in a lying position. During the time-domain analysis of HRV, differences between adjacent normal R-R intervals more than 50 milliseconds, expressed as a percentage, and differences between the coupling intervals of adjacent normal RR intervals increased in the lying position (p < 0.001). These results as a whole suggest that parasympathetic nervous system activity increases with fatigue.

Adult↗

PubMed2004

EEG and ECG changes during selected flight sequences.

BACKGROUND: Mental workload has become a critical factor in the design and use of modern aircraft. Because of the complexity of the human-machine system, it is necessary to determine workload, fatigue, and level of performance using noninvasive electrophysiological measures. OBJECTIVE: Our objective was to identify the electrophysiological indicators of mental workload during piloting tasks. METHODS: Electroencephalographic (EEG) and electrocardiographic (ECG) activity was recorded during actual flight, with a profile planned to produce different levels of mental workload. RESULTS: In-flight EEG and ECG recordings enabled us to document mental workload levels. During active segments, delta and theta band activity increased (p < 0.05 or greater); results showed an increase of 22.5% for theta band activity during active flight segments compared with in-flight rest periods. Inversely, alpha band activity diminished: the decrease between ground baseline and all flight sequences was 30% (p < 0.05 or greater). These variations were reversed during the in-flight rest sequences. Instrumental flight caused an increase in the theta and alpha frequency band activity in the parietal-occipital area (p < 0.05 or greater); the alpha/beta ratio also increased. Heart rate increased during the active segments and fell during the in-flight rest periods. The mean difference between active segments and in-flight rest periods was of 8.89 bpm (i.e., an increase of 11.5%; p < 0.01). Heart rate was correlated to the EEG activity of the delta and beta bands in the central area (C3, Cz, C4, p < 0.05). CONCLUSIONS: The electrophysiological measures recorded provide useful indicators of the mental workload required by different flight sequences.

Adult↗