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Biomedical subjects

Ji-yan Zheng

Publications and source records attributed to Ji-yan Zheng.

8 recordsLinked to original sources
PubMed2005

[Effects of phytoestrogens on gap junctional intercellular communication].

OBJECTIVE: To observe effects of phytoestrogens quercetin (QC), Genistein (GEN), coumestrol (COM), and enterolactone (ENL) on gap junctional intercellular communication (GJIC) in HaCaT cells. METHODS: HaCaT cells were exposed to QC, GEN, COM, and ENL at 0.1, 1.0, 10.0 and 100.0 micromol/L for 24 hours. The effects of phytoestrogens on GJIC were determined by fluorescence redistribution after photobleaching (FRAP) technique of using a laser scanning confocal microscope (LSCM). RESULTS: QC did not affect the GJIC at 0.1-10.0 micromol/L, whereas, GEN, COM, and ENL exhibited inhibition on the GJIC in some extent at 0.1-10.0 micromol/L without showing significant cytotoxicity. The ratio of fluorescence recovery were between 31.77% to 37.06%, which were significantly decreased compared the vehicle control (44.74%). CONCLUSION: The phytoestrogens GEN, COM, and ENL, but not QC, could inhibit the GJIC function in HaCaT cells at concentrations could be reached in human serum in some instance, indicating they could, under certain conditions, be cancer promoters. Therefore, it should be prudent to use these chemicals as pharmaceuticals or dietary supplements.

Cell Communication↗
PubMed2003

[Relationship among serum cortisol and insulin and blood glucose in the asphyxiated neonates].

OBJECTIVE: To explore the dynamic change of serum cortisol and insulin levels, and their relation with blood glucose concentration in asphyxiated neonates. METHODS: The levels of serum cortisol and insulin at d1,d3 and d7 of birth were measured by radioimmunoassay and the concentration of blood glucose was measured with glucose oxidase method in 43 asphyxiated neonates. RESULTS: The levels of serum cortisol at d 1, d 3 and d 7 of birth were gradually decreased (P<0.01). At d1, the incidence of hyperinsulism (>20 mIU/L) was 60.5%. The level of serum insulin reached normal level (<or=20 mIU/L) at d 3. At d 1, the level of serum cortisol was positively related with the level of blood glucose (r=0.432, P<0.01), the ratio of serum insulin and cortisol in hypoglycemia group (0.71+/-0.71) was significantly higher than that in normal blood glucose group (0.19+/-0.36, P<0.01). The incidence of hypoglycemia in hyperinsulism group was markedly higher than that in normal serum insulin group (P<0.05). CONCLUSION: There are temporary hyperinsulism in asphyxiated neonates. Hypoglycemia in asphyxiated neonates is related with hyperinsulism and low serum cortisol level.

Asphyxia Neonatorum↗