Biomedical subjects

Jia Na

Publications and source records attributed to Jia Na.

9 recordsLinked to original sources

PubMed2005

[Neurolymphomatosis, a case report].

Neurolymphomatosis (NL) is characterized by lymphomatous infiltration of the peripheral nervous system. We report a case of neurolymphomatosis (NL) which was confirmed by sural nerve biopsy. Sural nerve specimen from a 49-year-old female patient with weakness of limbs was examined with routine histochemical and immunohistochemistry staining, in which the first antibodies against CD3, CD20, CD45, CD45RO and CD68 were used. Numerous T-lymphoma cells invaded in the adipose tissue of epineurium of sural nerve. The nerve biopsy showed marked axonal degeneration of myelinated fibers. The clinical and histopathologic findings confirmed the diagnosis of neurolymphomatosis.

Female↗

PubMed2004

[Histologic features and pathologic diagnosis in usual interstitial pneumonia].

OBJECTIVE: To study the pathologic features, differential diagnosis and role of open lung biopsies (OLB) in usual interstitial pneumonia (UIP). METHOD: The authors reviewed the pathologic, clinical and radiologic features of five cases of UIP (one autopsy case and four OLB cases), with follow-up information. RESULTS: The typical histologic features were a non-uniform distribution of alveolar inflammation, fibroblastic foci, interstitial fibrosis and honeycomb change. There also was associated metaplasia of bronchiolar epithelium, type II pneumocyte hyperplasia and accumulation of alveolar macrophages. CONCLUSIONS: Characteristically, UIP exhibits temporal heterogeneity under low-power light microscopy, which includes changes in both the early and end stages. Open lung biopsy is an important diagnostic adjunct for suitable patients with atypical radiologic features on computerized tomography. Correlation between clinical, radiologic and pathologic findings is also essential for a correct diagnosis.

Aged↗

PubMed2003

[Plain radiograph and CT features of 112 patients with SARS in acute stage].

OBJECTIVE: To retrospectively analyze the X-ray and CT features of patients with suggestive of SARS at the early stages. METHODS: The study comprised 112 patients (43 men, 69 women; aged from 16 to 82 year) with clinically proved SARS. Chest radiograph was performed in all the patients and CT of thorax in 22 patients during the first week of the onset. RESULTS: In the early stage, the most common findings were patchy ground-glass opacification (GGO) (79.5% on X-ray and 81. 8% on CT) and mixed lesions of GGO with irregular consolidation (21.4% on X-ray and 45.5% on CT) and/or granulomas (14.3% on X-ray and 27.3% on CT). A majority of lesions were located in the middle and lower field (70.9%) with peripheral distribution (69%). Other findings on CT included intralobular and interlobular septal thickening (carzy-paving pattern) (36.4%), bronchiectasis (18.2%) and small pleural effusion (22.7%). CONCLUSION: Common radiologic findings of SARS are ground-glass opacification and mixed lesions of GGO with irregular consolidation and/or granulomas and lower location and peripheral distribution.

Adolescent↗

PubMed2003

[Prognostic significance of Ki67 and p53 protein in small bowel stromal tumor immunopositive for CD117].

OBJECTIVE: To investigate the correlative factors of the prognosis by observing the immunohistochemical features of small bowel stromal tumors(SBST). METHODS: Thirty-three cases of stromal tumors with expressed CD117 were examined by the light microscope. The expressions of Ki67 and p53 protein with EnVision + staining were detected in the tumors and compared with the morphology. RESULTS: The group included 20 males and 13 females whose ages ranged from 21 to 71 years (median: 46 years); the tumors were situated in duodenum (5 cases), jejunum (22 cases) and ileum (6 cases). Grossly, tumors size was from 1.5 to 20 cm. In eleven cases tumors showed foci of tumor cell necroses. In all these cases, mitotic counts ranged from 0 to 62 mitotic figures per 50 HPF (high-power fields), six of 33 tumors had no mitotic figures per 50 HPF, 5 cases had 1-2 mitotic figures and 22 cases had > or = 3 mitotic figures. Histologically, 4 cases classified as benign, 4 cases as borderline and 25 cases as malignant. Follow-up of 7 cases with benign and borderline tumors were alive without tumor. In the malignant group of 23 cases, 6 patients died of disease, 10 patients were alive with evidence of metastatic disease, and 7 patients were alive without tumor. Immunohistochemically, 31 of 33 stromal tumors were positive for Ki67, 16 cases of malignant tumors showed significant staining (> 5% cells). 31 of 33 tumors were positive for p53 protein, and all the 25 (100%) malignant tumors showed staining. In 12 of the 25 cases, the staining was strong and diffuse(> 50% cells). When the Ki67 and p53 proteins were negative or showed focal expressions (Ki67 < 1% and p53 < 10%), the patients presented a good prognosis. And when there were strong expressions (Ki67 > 5% and p53 > 50%), the patients portended a poor prognosis. CONCLUSION: Immunohistochemical analysis of Ki67 and p53 protein was a better method in the prognosis. When Ki67 showed strong position, it was a bad sign. When p53 protein showed strong position, it might be the potential malignant parameter. When Ki67 and p53 proteins were all strong expressions, these patients portended a poor prognosis. In addition to the invasion and metastasis, tumor size, mitotic counts and tumor cell necrosis were also helpful parameters for diagnosis of malignancy.

Adult↗

PubMed2003

[Pathologic diagnosis of primary small round cell tumors of the bone].

OBJECTIVE: To study the diagnosis and differentiation of primary small round cell tumors of bone. METHODS: Thirty-nine cases were studied by light microscopy and immunochemistry of O13, NSE, S-100, Actin and LCA. RESULTS: 20/22 cases of EW/PNET were positive for O13.16/22 cases of EW/PNET and one case of small cell osteosarcoma were positive for NSE. S-100 was positive in EW/PNET, small cell osteosarcoma and mesenchymal chondrosarcoma. All malignant lymphomas were positive for LCA. 6 cases of small cell osteosarcoma have osteoid production. Islands of cartilage were found in mesenchymal chondrosarcoma. CONCLUSION: O13 is a specific valuable marker for the diagnosis of EW/PNET. Histologic features of the osteoid in osteosarcoma and the cartilage in mesenchymal chondrosarcoma are important for the diagnosis.

Adolescent↗

PubMed2003

[A clinicopathological study of Castleman's disease].

OBJECTIVE: To study the pathogenesis and the differential diagnosis of Castleman's disease. METHODS: Histopathology, immunohistochemical staining and clinical courses of 26 cases of Castleman's disease (CD) were studied with follow-up study of 16 cases. RESULTS: The present study included 6 cases of multicentric type, 20 cases of localized type in the clinical aspects and 19 cases with hyaline vascular type, 4 cases with plasma cell type, 3 cases with mixed type in the histologic aspect. The Multicentric type presented systemic lymphadenopathy, anemia, hyperglobulinemia, hepatosplenomegaly, skin changes, and lung disorder and kidney disfunction, of which 1 case died of respiratory and renal insufficiency. 13 of the 20 localized cases were of the hyaline vascular type, and with good prognosis. 7 of the 20 cases showed paraneoplastic pemphigus associated with hyperglobulinemia (4/7) and lung disease (5/7). The pathologic features composed of proliferation of the mantle zone B cell, follicular dendritic cell, plasma cell and small vessels. In immunohistochemical staining, kappa and lambda light chains were detected in each CD case. CONCLUSIONS: Many diseases are similar to CD clinicopathologically. It is important to make differential diagnosis through pathological study. Castleman's disease is a lymphoproliferative disorder. The pathogenesis of this multicentric disorder may be associated with autoimmune disease.

Adolescent↗

PubMed2002

[Localized Castleman disease with paraneoplastic pemphigus and pulmonary involvement: clinical features and histopathology].

OBJECTIVE: To investigate the clinical, histopathologic and CT features of localized Castleman disease complicated with paraneoplastic pemphigus (PNP) and pulmonary involvement. METHODS: The clinical, laboratory, histopathologic records and data of computed tomographic (CT) images of 4 patients of Castleman disease with PNP were reviewed. RESULTS: All of the four patients presented with PNP which was confirmed by pathological and immunological studies and also developed lung abnormalities, including bronchiolitis obliterans (n = 3), lung abscess (n = 1) and multiple lung infarction (n = 1), 2 to 5 months after the onset of PNP. Castleman's tumor, which was found incidentally during routine examination, manifested as a solitary and huge mass (5 to 14 cm in size) which markedly enhanced homogeneously on CT in the retroperitoneum (n = 3) and mediastinum (n = 1). Histologically, three cases were of hyaline-vascular type and the other one was of mixed type. Complete remission of PNP occurred 2 weeks to 8 months after surgical resection of the tumor. The condition of two patients with bronchiolitis obliterans failed to be improved. They had been presenting dyspnea and irritable cough 3 to 15 months after surgery. CONCLUSION: PNP and lung abnormalities are rare and severe complications of localized Castleman's disease. Early diagnosis and surgery are very important for prognosis.

Adolescent↗

PubMed2002

Study of gastrointestinal stromal tumors by light microscopy, electron microscopy and immunohistochemistry.

OBJECTIVE: To investigate the morphological features, immunohistochemical speciality of the gastrointestinal stromal tumors (GISTs), and its histogenesis as well. METHODS: The morphologic characteristics of GISTs were studied in 65 cases using light microscopy and 17 cases by electron microscopy. The expression of c-kit (CD117), CD34, vimentin, SMA, actin, desmin, S-100 and MBP were detected in all the cases with EnVision trade mark staining. RESULTS: Among 65 cases of GISTs, 46 were spindle cell type, 6 epithelioid cell type and 13 mixture type, equivalent to 85.5% (65 of 76) of all of the mesenchymal tumors of gastrointestinal tract admitted in the same period. The epithelioid cell type tumors composed of mainly the epithelioid cells, predominantly short spindle, oval or round in pattern, with an overall eosinophilic cytoplasm by hematoxylin-eosin stain. Focal cytoplasmic vacuolization was often seen. Sometimes signet-ring like cells and cells with a clear cytoplasm were seen in the epithelioid stromal tumor. The tumor cells arranged in interlacing fascicles forming whorls or sometimes cell clusters. Electronic microscopy showed the presence of interdigitating cell processes, in some areas, synapse-like structure and numerous desmosome-like junctions as well as a few gap junctions and small round neurosecretory granules. There were also abundant intermediate filaments and thin filaments of actin-type with longitudinal condensations (dense bodies). All of the 65 stromal tumors were strongly positive for vimentin (100%), 61 out of 65 tumors positive for CD117 (c-kit) (93.8%) and 51 out of 65 positive for CD34 (78.5%). Some cases also expressed SMA, actin, S-100 and MBP. CONCLUSIONS: GISTs were the most frequent mesenchymal tumor seen in the gastrointestinal tract. Under light microscope, the morphology of stromal tumors looks sometimes like a leiomyoma and Schwannoma. The application of immunohistochemical markers (particularly CD117 and CD34) and ultrastructural study are considered necessary for the differential diagnosis. GISTs may originate from the pluripotential precursor cells like the interstitial cells of Cajal.

Gastrointestinal Neoplasms↗

PubMed2002

[A study of the expression of CD(44) in pneumocytes in pulmonary sarcoidosis].

OBJECTIVE: To study the expression of CD(44) in pneumocytes in pulmonary sarcoidosis. METHODS: CD(44) and AE(1)/AE(3) were detected by immunostaining in lung tissues from 21 cases of pulmonary sarcoidosis, 5 cases of tuberculosis, 4 cases of idiopathic interstitial fibrosis, 3 cases of Wegener's granulomatosis and 8 normal controls. The numbers of CD(44) positive cells and AE(1)/AE(3) positive cells (total alveolar epithelial cells) per three high-power fields were counted, and the ratio of CD(44) cells to AE(1)/AE(3) cells (84 +/- 6) were calculated. RESULTS: The total number of alveolar epithelial cells (110 +/- 32) and the density of CD(44) cells were significantly higher in sarcoidosis than in the normal lungs. CD(44) (18 +/- 4) staining was localized in the pneumocyte membrane and the positive cells were mostly type II pneumocytes as confirmed by histology. CONCLUSION: Proliferation of pneumocytes and increased expression of CD(44) in pneumocyte membrane, especially in the type II pneumocytes, may play an important role in lung inflammation and repair in pulmonary sarcoidosis.

Adult↗