Jia-jun Wang | PubMed Health

PubMed2006

[The quality assessment of cigarette paper by SIMCA and PLS combined with near infrared spectrum].

By using algorithm of SIMCA and partial least squares(PLS) combined with Fourier transform near infrared spectra (FT-NIR), the classification methods were established for the discrimination of cigarette paper. Meanwhile, the calibration models were established for the determination of the grammage, thickness, permeability, moisture and ash of cigarette paper. Correlation coefficients of the models were 0.9768, 0.9664, 0.9470, 0.956 3 and 0.9759, and the root mean square errors of cross validation (RMSECV) were 0.5614, 0.0960, 1.2741, 0.0967 and 0.2603 respectively. The methods has been applied to the determination and discrimination of unknown samples with satisfactory results.

English Abstract↗

PubMed2005

[A study on the inhibitory effect on transplanted tumor growth expressing HCV NS3 protein by HCV minigene vaccine based on invariant chain substitution in mice].

OBJECTIVES: To establish a transplanted tumor producing HCV NS3 protein in mice and study the therapeutic effect of minigene vaccine based on invariant chain substitution. METHODS: SP2/0-NS3 cells expressing HCV NS3 antigen were injected subcutaneously into BALB/ c mice. After three days of inoculation, different therapeutical reagents were injected intramuscularly into different groups of mice. The boost immunization was carried out two weeks after the first immunization. The efficiency of HCV NS3 Th1 minigene vaccine was estimated after 60 days observation. RESULTS: For saline, pCI-neo, pHCV-NS3 and pHCV-NS3-Th1 treated groups, the induction period needed for tumor growth was 16.17+/-2.55, 14.40+/-1.82, 16.75+/-2.36, and 24.00+/-5.57 days (t =2.623, P =0.034 vs saline, t =3.713, P =0.010 vs pCI-neo and t =2.425, P =0.045 vs pHCV-NS3) respectively. The tumorigenesis rates were 100%, 100%, 57.1% (8/14, chi2 = 6.190, P = 0.013 vs saline and chi2 = 6.608, P = 0.010 vs pCI-neo) and 46.7% (7/15, chi2 = 9.707, P = 0.002 vs saline and chi2 = 10.311, P = 0.001 vs pCI-neo ) respectively. The survival rates were 0, 0, 50.0% (7/14, chi2 = 5.787, P = 0.016 vs saline and chi2 = 9.333, P = 0.002 vs pCI-neo) and 53.3% (8/15, chi2 = 6.651, P = 0.010 vs saline and chi2 = 10.311, P = 0.001 vs pCI-neo) respectively. The average tumor diameter of the pHCV-NS3-Th1 treated group was significantly smaller compared with the control groups and the pHCV-NS3 treated group (P =0.001). Moreover, the average survival time of tumor-bearing mice immunized with pHCV-NS3-Th1 was 6 days longer compared with the saline treated group, 12 days longer compared with the pCI-neo treated group (P =0.001), and 6 days compared with the pHCV-NS3 treated group. CONCLUSION: HCV NS3 Th1 epitope vaccine might be a potential biotherapy candidate against HCV infection.

Animals↗

PubMed2003

[Interaction between polyamide and zinc salt].

X-ray diffraction and FTIR spectroscopic method have been used to investigate the interaction between the amide group of nylon 6 and zinc chloride. The nylon sample obtained by adding formic acid solution of nylon into aqueous solution saturated by zinc chloride is significantly different from the nylon precipitated after mixing formic acid solution of nylon and water. X-ray diffraction results demonstrate that nylon precipitated from water has crystallization while the sample acquired from saturated solution of ZnCl2 remains amorphous. FTIR spectroscopic study shows that the interaction between zinc ion and the amide group of nylon 6 brings about significant variation of amide I and II bands and prevents the crystallization of nylon. The interaction between nylon and zinc ions may be helpful in the development of a new method of manufacturing new nylon material with superior performance.

Chlorides↗