Biomedical subjects

Jian-Hua Piao

Publications and source records attributed to Jian-Hua Piao.

4 recordsLinked to original sources

PubMed2006

[Comparison of transferrin receptor between child-bearing period women and children].

UNLABELLED: To compare plasma TfR, ferritin concentrations and their ratios in children and child-bearing period women and look for correlation between TfR concentration and other measures of iron status. METHODS: Our study groups were healthy 3-6-year-old preschool girls (n = 109), 11-12-year-old prepubertal girls (n = 133) and 20-45-year-old childbearing period women (n = 115). Plasma TfR concentration, SF concentration and TfR/SD were compared in different groups. The correlation of TfR and other indexes were studied. RESULTS: TfR concentrarions were higer in preschool girls [(23.34 +/- 6.78) nmol/L] than in prepubertal girls [(21.33 +/- 5.30) nmol/L] and higher in prepubertal girls than in childbearing period women [(19.86 +/- 4.83) nmol/L]. SF concentrations were higher in prepubertal girls [(60.37 +/- 33.39) microg/ L] than in child-bearing women [(57.17 +/- 29.81) microg/L] and higher in child-bearing women than in preschool girls [(47.83 +/- 24.49) microg/L]. TfR/SF ration were higer in preschool girls (0.72 +/- 0.83) than in prepubertal girls (0.48 +/- 0.47) and higher in prepubertal girls than in child-bearing period women (0.47 +/- 0.37). By multiple regression analysis, the predictors of TfR concentration were plasma ferritin, hemaglobin and age. CONCLUSION: Plasma ferritin, Hb and age, even within the normal physiologic SF, Hb, influence TfR concentrations. The lower the iron stores, the stronger the influence of ferritin on TfR. The TfR concentrations are higher in children than adults respongsing to physiologically low iron stores in children.

Adult↗

PubMed2006

[Assessment methods and evolution on vitamin A status].

Vitamin A is one of nutrients which are indispensable to all people, especially children and pregnant women. In the world, severe vitamin A deficiency (VAD) has been controlled efficiently. But to many countries, especially those developing, subclinical or marginal vitamin A deficiency is still a public health problem. It is very important and necessary to assess the status of vitamin A of the population who are in risk of VAD before drawing and carrying out the intervention strategy. Currently, clinical, histological, functional and biochemical methods can be used to evaluate the level of vitamin A.

Animals↗

PubMed2005

[Study on the food safety of the marker gene expressed protein HPT].

OBJECTIVE: This article studied on the food safety of HPT protein (Hygromycin B Phosphotransferase), a kind of plant selectable marker gene expression product, which mainly included the quantity and quality detection of HPT in the SCK transgenic rice plants, the possible dietary intakes and its stability in the simulative digestive system. METHODS: HPT cDNA fragment was inserted into the prokaryotic expressing vector pBV222 for the expression of 6His-HPT fusion protein. The inclusion bodies were denatured by 8 mol/L urea and purifiee with metal chelate affinity chromatography on Ni-NTA agarose under denaturing condition. The purified 6His-HPT were used to immunize rabbit and BALB/C mice for the preparation of the polyclonal and monoclonal antibodies, and the double-Ab sandwich ELISA to detect HPT was established then. Concerning the sensitivity assessment of HPT protein, the amino acid sequences of HPT was compared with the other allergens and the simulative digestive experiment in vitro was carried out too. RESULTS: 6His-HPT protein was obtained with the purity up to 95%. The specificity of antisera and ascites was detected by Western blot, which showed specific binding reaction between the antibodies to the purified 6His-HPT proteins and their expressed product. The double-Ab sandwich ELISA system was established successfully with the sensitivity of 30 ng/ml. The HPT expression level in the leaves of transgenic rice was proved in the range of 80 - 150 ng/ml, yet we cannot detect HPT protein in the seeds of the same rice. There showed no comparability in the amino acid sequences between HPT and other allergens, and HPT protein was digested quickly in the simulated stomach and intestines liquids. CONCLUSION: All the results showed that the expression level of HPT in the SCK transgenic rice plants is very low and they are not stable in the simulative digestive system. So we inferred that under the current detection level, HPT protein might not be able to induce any safety problem. Yet further study on the acute toxicity and sensitivity experiments by the animals need to be carried out, and the results will be reported in the near future.

Animals↗

PubMed2005

Disease risks of childhood obesity in China.

OBJECTIVE: To estimate the relative risks of dyslipidemia, hypertension, diabetes mellitus, and metabolic syndromes among overweight and obese Chinese children compared with their normal weight counterparts. METHODS: Overweight and obesity were defined by age- and sex-specific BMI classification reference for Chinese children and adolescents. Pediatric metabolic syndrome (MetS) and each risk factor for MetS were defined using the criteria for US adolescents. Definition of hyper-TC, LDL, and dyslipidemia for adults was applied as well. General linear model factor analysis and chi-square test were used to compare the difference in metabolic indicators among normal weight, overweight, and obese groups. Multiple logistic regression analysis was performed to estimate the odds ratio of metabolic abnormalities between obesity, overweight, and normal weight children, after adjustment for living area, family economic level, age, sex, and daily exercise time and TV watching time, as well as different dietary indices in the model. RESULTS: Significant increases in blood lipids, glucose, and blood pressure were found among overweight and obese children as compared with their counterparts with normal weight. By applying WGOC-recommended BMI classification, the risks for hypertriglyceridemia, low HDL and dyslipidemia among overweight children were 1.9, 1.4, and 1.5 times, and was 3.3, 1.5, and 1.8 times among obese groups compared to their counterparts with normal weight after adjustment for age, sex, region, socioeconomic status, physical activity, and dietary intakes. The overweight and obese children (15-17.9 years) had a high-risk of developing hypertension, which was 2.3 and 2.9 times higher than their counterparts with normal weight. Above 90% obese adolescents had abdominal obesity, while less than 1% normal weight ones had abdominal obesity. No obese adolescents were free from any risk factors for MetS, while 36.9% of normal weight adolescents were from the risk factors. 83.3% obese boys and all obese girls had metabolic syndrome, while only 15.5% normal weight boys and 18.8% normal weight girls had metabolic syndrome. Four risk factors for metabolic syndrome were found in 8.3% obese boys while none in normal weight boys and girls. The prevalence of MetS among normal weight, overweight, and obesity groups was 1.5%, 18.3%, and 38.1% respectively. CONCLUSION: The cardiovascular disease (CVD) risk factors are clustered in obese Chinese children. Our observations strongly suggest that efforts should be made to prevent the onset of overweight and its associated diseases during early childhood.

Adolescent↗