Biomedical subjects

Jian-Jun Yu

Publications and source records attributed to Jian-Jun Yu.

4 recordsLinked to original sources

PubMed2005

[Kidney transplantation improves spermatogenic function in patients with uremia].

OBJECTIVE: To study the changes of testicular spermatogenic function after kidney transplantation. METHODS: Thirty male patients with uremia underwent kidney transplantation. Semen analyses were made and sperm ultrastructural changes were studied before and after the operation. RESULTS: Sperm viability, motility and movement velocity were significantly higher in the postoperative group than in the preoperative group [(70.08 +/- 2.86 vs (36.04 +/- 8.01)], [(76.15 +/- 3.75)% vs (39.27 +/- 4.14)%], [(16.80 +/- 1.11) microm/s vs (8.43 +/- 2.01)] micro/s, respectively (P < 0.01). Sertoli cell, acrosome and mitochondria structures inclined to normal after kidney transplantation. CONCLUSION: Kidney transplantation can significantly improve spermatogenic function in patients with uremia.

Adult↗

PubMed2002

[A comparative study of computer assistant sperm analysis with rontine sperm analysis]

OBJECTIVE: To compare computer assisted sperm analysis (CASA) with routine sperm analysis (RSA) in evaluation of male fertility. METHODS: The results of CASA were compared with the RSA of 27 males normal fertility and 317 males with infertility. RESULTS: Using the CASA, deformity rate was lower than RSA, it was 25.10+/-8.57 and 31.48+/-12.81 in the fertile and infertile groups respectively, whereas, RSA was 35.56+/-9.58 and 54.56+/-13.47. Fertile group motility had a significant difference, it was 61.35+/-7.62 and 83.96+/-6.67. Sperm vitality of grades A was 9.10+/-5.50 and 30.33+/-8.85. The vitality of grades B was 31.16+/- 12.35 and 49.93+/-8.56, the vitality of grades C awas 21.06 +/-14.19,and 3.70+/-2.12, the vitality of grades D was 38.66+/-7.62 and 16.04+/-6.67. When two techniques were adopted, the mobility had no difference in infertile group (42.53+/-14.95 and 43.09+/-13.96), the sperm vitality was decreased in grades B (CASA 22.67+/-14.96,RSA 33.32+/-13.47), increased in grades C(CASA 17.08+/-13.47,RSA 7.68+/-5.82), they had significant differences (P<0.01). The grade B sperms in the non-forward moving group, CASA was 2.77+/-6.01, RSA 0. In addition, VSL, VCL, VAP, ALH, sperm trace and static diagram were adopted. CONCLUSION: Using the CASA,the distinguished ability to sperm shape and vigor was lower than RSA, but the quantize marker (such as straight line velocity, curved velocity, average path velocity and ALH) and moving, static images could reflect on sperms quality more objectively.

Journal Article↗

PubMedDate not supplied

Ultrastructural defects of acrosome in infertile men.

To study the ultrastructural changes of acrosomes in fertile and infertile males and to identify the morphological alterations closely related to male infertility, forty spermatozoa from 8 fertile males and 60 spermatozoa from 12 infertile males were collected and observed by scanning electron microscope. There were significant changes in the morphology of the circular sulcus, acrosome membrane and head of the infertile sperm. The circular sulcus of spermatozoa was more serrated (39/60 vs 2/40) and blurred (40/60 vs 4/40) in the infertile group than in the fertile group. The acrosome of infertile group occupied a bigger proportion of sperm head (70+/-8% vs 57+/-6%) than in the fertile group. The acrosome membrane in the infertile group was less intact (18/60 vs 31/40) and less smooth (19/60 vs 32/40) than in the fertile group. More droplets were attached to the acrosome membrane in the infertile (39/60 vs 11/40) than in the fertile groups. The abnormality in acrosome ultrastructure seems to be significantly higher in infertile than in fertile males, suggesting a potential role of such defects for male fertility defect.

Acrosome↗

PubMedDate not supplied

Decreased testicular expression of cAMP response element modulator (CREM) in rat with varicocele.

This study examines the hypothesis that varicocele would impair the testicular expression of cAMP response element modulator (CREM) in experimental rats. Thirty (30) rats were selected at random, of 20 were operated as varicocele's group; of 10 were for sham-operation as controlled group, testes were removed, fixed and stained in three months. Makler Score was adopted to analyze the bore, limitan's thickness, layer number of cell, the degree of cell's maturity and average score in 200 and 100 seminiferous tubulae, respectively. CREM, HSP60 was determinated by hybridization in situ, the difference between two groups were compared. In varicocele group, seminiferous tubule's bore was decreased (101 +/- 2.2) vs 146 +/- 4.1), limitan became thicker (3.5 +/- 0.1 vs 1.9 +/- 0.2), cell's layer number was reduced (3.0 +/- 0.2 vs 5.5 +/- 0.1), cell's maturity turned to disturbance (3.6 +/- 0.3 vs 4.9 +/- 0.1), the average score was lower than controlled group (8.5 +/- 0.6 vs 16.0 +/- 1.2), they had significant differences (P < 0.001). The testicular CREM expression was significantly lower in varicocele group than in controlled one (VG2.0 +/- 0.32, SoG3.90 +/- 0.32) (p < 0.001), which was located in spermatogenous cell to spermatocyte stage. However, HSP60 expression in VG was higher than in SoG (3.85 +/- 0.3 versus 2.1 +/- 0.32) with significant differences (P < 0.001), the expression located in spermatid. Varicocele could lead to lower testicular CREM expression and breeding sperm functional lesion.

Animals↗