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Jian-Min Wan

Publications and source records attributed to Jian-Min Wan.

At least 19 recordsLinked to original sources
PubMed2006

SSR mapping of brown planthopper resistance gene Bph9 in Kaharamana, an Indica rice (Oryza sativa L.).

The brown planthopper (BPH) is one of the most serious insects pests of rice, and the host resistance has been recognized as one of the most economic and effective measures for BPH management. In this study, we conducted a molecular-based genetic analysis of Bph9 in Kaharamana, a Sri Lanka rice variety resistant to BPH insects of East and Southeast Asia. An F2 segregating population composed of 180 plants was constructed from the cross between Kaharamana and 02428, and each F2 plant was self-crossed to obtain F2:3 family. The bulked seedling test method was used to evaluate the resistance of F2:3 families, and the genotype of each F2 plant was inferred from the phenotype of corresponding F2:3 family. Linkage analysis indicated that the resistant gene Bph9 in Kaharamana was located between SSR markers RM463 and RM5341 on chromosome 12 with linkage distances of 6.8 cM and 9.7 cM, respectively. The time- and money-saving SSR markers would be helpful in the application of Bph9 in breeding program via marker-assisted selection.

Animals↗
PubMed2006

Mapping and marker-assisted selection of a brown planthopper resistance gene bph2 in rice (Oryza sativa L.).

Nilaparvata lugens Stål (brown planthopper, BPH), is one of the major insect pests of rice (Oryza sativa L.) in the temperate rice-growing region. In this study, ASD7 harboring a BPH resistance gene bph2 was crossed to a susceptible cultivar C418, a japonica restorer line. BPH resistance was evaluated using 134 F2:3 lines derived from the cross between "ASD7" and "C418". SSR assay and linkage analysis were carried out to detect bph2. As a result, the resistant gene bph2 in ASD7 was successfully mapped between RM7102 and RM463 on the long arm of chromosome 12, with distances of 7.6 cM and 7.2 cM, respectively. Meanwhile, both phenotypic selection and marker-assisted selection (MAS) were conducted in the BC1F1 and BC2F1 populations. Selection efficiencies of RM7102 and RM463 were determined to be 89.9% and 91.2%, respectively. It would be very beneficial for BPH resistance improvement by using MAS of this gene.

Animals↗
PubMed2006

[Physical localization of ribosomal genes and chromosome DAPI banding by in situ hybridization in Medicago sativa L].

Physical localization of ribosomal genes in diploid and tetraploid alfalfa (Medicago. sativa) was studied using fluorescent in situ hybridization (FISH). It was revealed that 45s gene was only located at nucleolus organizer region (NOR) with a single locus in both diploid and tetraploid alfalfa, while 5s gene had 2-3 loci on chromosomes. Using the genomic DNA from M. coerulea and M. falcata as probe to hybridize with tetraploid species in alfalfa, both diploid species were successfully hybridized with tetraploid chromosomes, only showing the difference in hybridization signals in different numbers of chromosomes. Chromosomes of alfalfa exhibited DAPI banding by FISH analysis. In general, the patterns of distribution of DAPI banding were consistent with C-banding for M. coerulea. The possible origination of tetraploid alfalfa was discussed based on DAPI banding patterns and FISH analysis for ribosomal genes .

Chromosome Banding↗
PubMed2006

[Inheritance, expression and armyworm resistance of protease inhibitor II gene (Pin II) driven by different promoters in transgenic rice].

The inheritance of rice lines transformed by protease inhibitor II gene under control of different promoters was investigated by analysis of hygromycin resistance, PCR and Southern blot. For segregation patterns of foreign gene, 68.4% of the transgenic rice plants were conformed to a Mendelian ratio and in which the rate of transgenic plants with single copy was 63.6%. Quantitative analysis of Pin II protein expressed in transgenic rice plants showed that Pin II protein in fresh leaves was 160 microg/g for Act-Pin II-2x, 176 microg/g for Ubi-Pin II-2x, and 104 microg/g for PIN5'-Pin II-4x separately while in control rice plants was only 20 microg/g. The inhibitory activity against tryspin of Pin II gene driven by Actl and Ubi promoter reached 37.7% and 43.1%, much higher than that driven by PIN5' (29.2%). Bioassay for insect resistance to armyworm (Pseudaletia separata Walker) revealed that transgenic plants had increased their resistance to the pest but there was not significantly different from controls, and also there was no correlation between insect resistance to armyworm and quantity of Pin II protein as well as promoters in transgenic rice.

Animals↗
PubMed2006

[The inductive activation of the promoter of pib gene].

A 5.7 kb putative promoter region of pib gene was isolated from the pib genomic clone and substituted for the 35S promoter upstream of gus gene in plasmid pCAMBIA1301 to construct a new plant expression vector pNAR604 (putative pib promoter-GUS + 35S-hpt). From Agrobacterium-mediated transformation and hygromycin selective culture in vitro, hygromycin resistant calli and 36 transgenic rice (Oryza sativa L.) plants were obtained. Histochemical assays of GUS activity showed that no expression was observed in the resistant calli and roots from transgenic rice if cultured under light, but after 24 h dark treatment there was strong GUS staining. Fluorimetric quantitative analysis indicated that GUS expression was organ-specific in transgenic rice. Without the dark treatment, GUS activity in roots and stems were about 7 and 3 times higher than in leaves in which GUS activity was only trace detected. After 24 h dark treatment, GUS activity in roots, stems and leaves of transgenic plants were all promoted and the largest increase was observed in leaves. Twenty-four hours after spraying with 5 mmol/L SA (Salicylic Acid) or 0.3 mol/L NaCl, GUS activity in leaves of the transgenic plants was 2.7 or 3.6 times respectively higher than untreated control. It was confirmed that an inductive promoter was involved in this 5.7 kb upstream region of pib gene, and dark, SA and NaCl treatments were inductive factors for pib promoter.

Carrier Proteins↗
PubMed2005

[Analysis of heading time genotype for a rice photoperiod and thermo--sensitive male sterile line PeiAi64S].

PeiAi64S, a photoperiod and thermo-sensitive genic male sterile, has been wildly applied to hybrid rice seed production in China, but its photoperiod-sensitivity gene for heading date in this sterile line was still unknown. This definitely limited the further use of this sterile line in breeding practice and re-production of hybrid seeds. To solve this problem, using heading time nearly isogenic lines EGO - EG7, ER - LR, T65 - T65m and six heading date QTL-isogenic lines, NIL (Hd1) - NIL (Hd6) with the genes of Nipponbare but Hd1 - Hd6 genes from Kasalath respectively, we performed a genetic analysis of PeiAi64S with special reference to photoperiod-sensitivity loci, in natural long days at Nanjing (32 degrees N) where the average day-length is about 14 h and in natural short days at Linshui county, Hainan province(18 degrees 29'N), where the average day-length is about 11.6 h during the course of rice growing respectively. The F1 and F2 generations from the crosses 'PeiAi64S x heading time nearly isogenic lines' were subjected to genetic analyses. Experimental results showed that PeiAi64S carries photoperiod-sensitivity allele gene E1 and E3 and dominant earliness gene Ef-1 in E1 and E3 and Ef-1 loci, respectively, and a photoperiod insensitivity allele Se-1 degrees in Se-1 locus. Meanwhile, the photoperiod-sensitivity gene E1 and photoperiod-insensitivity gene Se-1e in PeiAi64S were also identified by crossing with the NIL(Hd1) and NIL(Hd4). In addition, a recessive inhibitor for photoperiod-sensitive gene E1 or Se-1(n) and other modified photoperiod-sensitive genes: i-Se-1, E3, Hd3 (En-Se-1), Hd5 and Hd6, were identified in PeiAi64S by crossing with QTL nearly isogenic lines: NIL(Hd2), NIL (Hd3), NIL (Hd5) and NIL( Hd6), The results indicated that the genotype of PeiAi64S's heading date was: E1E1e2e2E3E3Se-1(e)Se-1(e)Ef-1 Ef-1 i-Se-1 i-Se-1.

Breeding↗
PubMed2005

[Cloning, Characterization and Expression Vector Construction of Potato Proteas-inhibitor II Gene (PIN II-2x) from Diploid Potato (Solanum phurejia).].

Based on the published gene sequence of tetraploid potato (Solanum.tuberosum) protease-inhibitor II, a genomic DNA and a cDNA sequence of potato protease-inhibitor II gene were obtained from the cDNA library and the genomic DNA of a diploid potato IVP101 (Solanum.phurejia) using PCR method and named PINII-2x. Nucleotide sequencing confirmed that the full-length DNA of PINII-2x was 580 bp, including an 115 bp intron and two exons. cDNA was 462 bp ( stop codon TGA not included) and had 88% similarity to the tetraploid potato protease-inhibitor II. The PINII-2x open reading frame encodes a 154-amino acid polypeptide with a predicated size of 16.6 KD and a calculated PI of 6.08. The deduced proteins from PINII-2x cDNA had 93% homology with other tetraploid potato protease-inhibitor II, which contain the intact signal peptide and two active site similar to the potato protease-inhibitor II family. Test of the RT-PCR indicated that PINII-2x mRNA is wound- induced expression in potato leaves. Binary vector of PINII-2x cDNA drove by either rice Actin I promoter (ActI) or maize Ubiquitin promoter (Ubi) was constructed.

Amino Acid Sequence↗
PubMed2005

[QTL analysis of low-temperature-sensitive pollen sterility in Indica-japonica hybrid rice (Oryza sativa L.)].

There existed a number of biological constraints in exploiting the heterosis of indica-japonica hybrid rice. The low-temperature-sensitive sterility (LTSS) of indica-japonica hybrid has become one of the major problems in indica-japonica hybrid rice breeding after the solution of poor fertility of the hybrids by the finding of wide-compatibility gene. Previous studies revealed that the LTSS might be caused by low-temperature-sensitive pollen sterility (LTSPS). However, the genetic basis of LTSPS remained unclear. To explore the genetic basis of LTSPS in indica-japonica hybrid rice, an F2 genetic population derived from 3037 (indica) and 02428 (japonica) was developed. At the booting stage, pollen fertility of F2 population together with parents were surveyed after the treatment with low temperature daily average of 21-23 degrees C. The linkage map was constructed containing 108 SSR markers distributed throughout the whole 12 chromosomes with average marker interval of 16.26 cM. Using software MapMaker/QTL, two putative QTLs, namely qLTSPS2 and qLTSPS5 on chromosomes 2 and 5 were detected by interval mapping, which could explain the phenotypic variation 15.6% and 11.9% respectively. The additive effects were 0.021 and 0.045, dominant effects were -0.246 and -0.215, and the degrees of dominance were 11.7 and 4.8, respectively for the two QTLs. Therefore, the mode of gene action in response to low-temperature stress was overdominance and LTSPS was mainly the result of interaction between the indica and japonica alleles within each locus. In addition, two-way ANOVA showed that the two QTLs acted essentially independent of each other in conditioning LTSPS.

Alleles↗
PubMed2005

[Genomic imprinting and seed development].

The endosperm, a seed tissue that mediates the transfer of nutrients from the maternal parent to the embryo, is an important site of imprinting in flowering plants. In Arabidopsis thaliana, three genes were identified that prevent fertilization-independent seed development: FIS1/MEDEA, FIS2 and FIS3/FIE. MEDEA (MEA), a master regulator of endosperm development, is known to be imprinted in the endosperm. FWA is also imprinted in the endosperm of the model plant Arabidopsis. The following aspects were included in the present review: the imprinting mechanism in angiosperms, the latest progress in the control of MEA and FWA imprinting, the parental conflict theory to explain imprinting, the imprinting methods and other imprinted genes found in plants.

Alleles↗
PubMed2005

[QTLs mapping and genetic analysis of tiller angle in rice (Oryza sativa L.)].

Tiller angle is one of the most important morphological characters that has a significant effect on the formation of rice high-yield population. The tiller angles were measured in a japonica/indica RIL population with 71 lines and a genome-wide chromosome segment substitution line (CSSL) population with 65 lines at two experimental sites. A transgressive segregation was observed in both populations. QTL analysis of tiller angle was conducted based on the saturated RFLP marker linkage map and the CSSL graphical genetype. Five main-effect QTLs and three pairs of epstatic loci were detected in the RIL population. A main QTL, qTA-9, located on chromosome 9 at XNpb108 - C506, was identified at both experiment sites. The positive allele TA-9(I) on qTA-9 was contributed by indica rice IR24 with 28.6% average contribution to variance. Meanwhile, an analysis of CSSL graphical genetypes also showed that there was a positive allele on the IR24 chromosome substitution segment delimited by RFLP marker C609 and C506 with approximate 15 cM interval, which proved the existence of qTA-9. The TA-9(I) could increase tiller angle by about 15 degrees in japonica Asominori background under the two environments. The measurement of the F1 from the cross between background parent and CSSL AIS68 with TA-9(I) and the analysis of F2 population indicated that the TA-9(I) was an incomplete dominant gene. Genetype x environment interaction(G xE) was not widely present except a pair of epistatic loci with 5.32% contribution to variance of tiller angles and a relatively small additive effect. The combining action of the additive effect of the genes from both parents and the two-loci epistasis-effect may be responsible for the transgressive segregation of tiller angle in rice population. The value and approach of application of TA-9(I) in hybrid rice breeding program were discussed.

Chromosome Mapping↗
PubMed2005

[QTL analysis for traits associated with photosynthetic functions in rice (Oryza sativa L.)].

A mapping population of 81 F11 lines (Recombinant Inbred Lines, RILs), derived from a cross between a japonica variety Kinmaze and an indica variety DV85 by single-seed descent method, was used to detect quantitative trait loci (QTL) for traits associated with photosynthetic functions. Total leaf nitrogen content (TLN), chlorophyll a/b ratio (Chl. a:b) and chlorophyll content (Chl) were measured in leaves of rice (Oryza sativa L.) at the 7th day after heading. A total of six putative QTLs were detected with percentage of variance explained (PVE) ranging between 11.2% -29.6%, and LOD of QTLs 2.66-4.81. Of those putative QTLs, three for TLN were detected on chromosomes 1,2 and 11, with PVE of 17.3%, 15.3% and 13.7%, respectively;Two controlling Chl. a:b on chromosomes 3 and 4, PVE of 13.8% and 29.6%, one for Chl on chromosome 1, PVE of 11.2%. Four of those detected QTLs were newly reported in this study. Interestingly, the QTL controlling chlorophyll content,namely qCC-1 reported here,was detected in the region of the RFLP marker C122 on chromosome 1, where harbored NADH-glutamate synthase structure gene according to a previous study. Because the biosynthesis of chlorophyll begins with glutamate, qCC-1 would play a vital role in photosynthetic functions. Whereas,no QTL controlling chlorophyll content were detected at the 30th day after heading, suggesting that the effect of the QTL controlling chlorophyll content decreased during leaf senescence.

Chlorophyll↗
PubMed2005

QTL for yield and its components responded to elevated CO2 in rice (Oryza sativa L.).

FACE (free air carbon dioxide enrichment) technology may provide a means by which the environment around growing plants can be modified to realistically simulate the concentration of atmospheric CO2 in the future. The plant growth and its yield of plant species can be enhanced under FACE. Identification of genomic regions influencing the response of yield and its components to elevated CO2 will be useful for understanding the genetics of active response to changed CO2 environment and developing higher yield cultivars, which will be adapted to future enriched atmospheric CO2 environment. A mapping population of 65 indica (IR24) chromosome segment substitution lines (CSSLs) in japonica (Asominori) background and their parents were used to detect QTLs for yield and its components, e. g. number of fertile tillers per plant (FT), 1000-grain weight (TGW), number of grains per panicle (GP) and grain yield per plant (GY) under FACE (200 micromol CO2/mol above current levels) and current CO2 concentration (Ambient, about 370 micromol CO2/mol) in the field experiment. The results showed that, GY, GP and FT of two parents under FACE were significant greater than that under Ambient. The transgressive segregation of the four traits was observed in the CSSLs population under both FACE and Ambient. A total of 20 QTLs for the four traits were detected on chromosome 1, 2, 4, 6, 7, 9 and 12 with LOD (Log10-likelihood ratio) of QTLs ranging from 2.5 to 5.7. Three QTLs were detected under both FACE and Ambient. However,other QTLs were detected only under one level of CO2, which indicated that these loci were sensitive to CO2 concentration. Additionally, two QTLs qFT12 and qGP4 were found for the QTL x Environment (QE) interaction effects. It is suggested that there is a high possibility to improve the yield of rice under elevated CO2 through marker-assisted selection.

Biomass↗
PubMed2005

[SSRHunter: development of a local searching software for SSR sites].

Progress in genome research has made it possible to develop new SSR markers by bioinformatics in a relatively narrow region of genome. To realize it, the first thing is to search for potential SSR sites. Any known methods have more or less defects. Efforts were made to develop a local SSR sites searching software in this study. The resultant software, SSRHunter, could accomplish this task perfectly. Furthermore, SSRHunter could provide automatic pretreatment of sequences, routine arrangement of sequences, sequence transformation and convenient report output.

Microsatellite Repeats↗
PubMed2005

Mapping of QTLS for ferrous iron toxicity tolerance in rice (Oryza sativa L.).

Ferrous iron toxicity is the main factor limiting the productivity of rice in gleyic paddy soils. In this study, an F2 and an equivalent F3 populations derived from a japonica/indica cross of rice, Longza8503/IR64, were raised under iron-enriched solution cultures, and used to map QTLs controlling ferrous iron toxicity tolerance. A genetic linkage map consisting of 101 SSR markers was constructed to determine the position and nature of quantitative trait loci (QTLs) affecting Fe2+ toxicity tolerance. Three characters, i.e., leaf bronzing index (LBI), plant height (PH) and maximum root length (MRL) were evaluated for the F2 plants and F3 lines and the parents at the seedling stage in nutrient solution. A total of 20 QTLs for LBI, PH and MRL under the Fe2+ stress were detected over 10 of the 12 rice chromosomes, reflecting multigenic control of these traits. QTLs controlling LBI were located at the region of RM315-RM212 on chromosome 1, RM6-RM240 on chromosome 2 and RM252-RM451 on chromosome 4. Compared with other mapping results: (1) the QTL for LBI located at the region of RM252-RM451 on chromosome 4 was identical with the QTL for decreased chlorophyll content on a rice function map. Another QTL for LBI located at the region of RM315-RM212 on chromosome 1 was linked with the QTL for chlorophyll content which located at the region of C178-R2635 on a rice function map. (2) The third QTL for LBI located at the region of RM6-RM240 on choromosome 2 was linked with the QTL for potassium uptake located at the region of RZ58-CDO686 under potassium deficiency stress.

Chromosome Mapping↗
PubMed2005

[Changes in some defensive enzyme activity induced by the piercing-sucking of brown planthopper in rice].

The effects of brown planthopper on the activities of some key enzymes involved in defense response and the H(2)O(2) content were investigated in rice. From the fact that after piercing-sucking of brown planthopper, malondialdehyde (MDA) content of rice plant was increased rapidly (Fig.3), one can conclude that it induced lipid peroxidation; lipoxygenase (LOX), hydroperoxide lyase (HPL) and phenylalanine ammonia-lyase (PAL) activities were increased significantly in plants of resistant and susceptible varieties by piercing-sucking of brown planthopper (Figs.1,2,4); whereas the catalase activity and the H(2)O(2) content were significantly different between resistant and susceptible plants by piercing-sucking of brown planthopper, the former being decreased and the latter increased in resistance plants of variety RH (Oryza sativa L. cv. Rathu Heenati), while the reverse was true in the susceptible plants of variety TN1 (Oryza sotiva L. cv. Taichung Native 1) (Figs.5,6). The effect of brown planthopper on the PAL activity and the H(2)O(2) content were systemic (Figs.4,6), but the effect on the LOX activity occurred only in stem, the organ which brown planthopper sucking (Fig.1).

Aldehyde-Lyases↗
PubMed2004

[QTL analysis for rice stripe disease resistance gene using recombinant inbred lines (RILs) derived from crossing of Kinmaze and DV85].

Rice stripe disease transmitted by small brown planthopper (Laodelphax striatellus Fall.) is one of the most serious viral diseases in East Asia. The disease is severely epidemic in most rice growing areas where the main cultivars are susceptible or moderately susceptible to rice stripe virus. In this research, a recombinant inbred lines (RILs) population of 81 lines derived from a cross of Kinmaze (japonica)/DV85(indica) by the single seed descent method was used to detect quantitative trait loci (QTL) conferring resistance to rice stripe virus(RSV). The response of the two parents and 81 RILs to RSV were investigated by inoculating seedlings with viruliferous small brown planthopper insects, and scored by the disease rate index. The quantitative trait loci for rice stripe disease resistance were analyzed by QTL Cartographer software. Three QTL controlling RSV resistance were detected on chromosomes 1, 7 and 11, respectively. Individual QTL accounted for 19.8%-30.9% of the phenotypic variance in the RILs population. The direction of the additive gene effects at two loci qStv7 and qStv11 coincided with that predicted by phenotypes of the parents. At these two loci, the DV85 alleles increased the resistance to RSV, while at qStv1, the Kinmaze alleles increased the resistance to RSV.

Chromosome Mapping↗
PubMed2004

Inheritance and QTL mapping of low temperature germinability in rice (Oryza sativa L.).

Quantitative trait loci (QTL) controlling low temperature germinability (LTG) in rice were identified using 81 recombinant inbreed lines (RILs) derived from a cross between a japonica variety Kinmaze and an indica variety DV85. The accurate condition of LTG evaluation was assumed at 15 degrees C for 10 d after removing the effect of dormancy and the second dormancy. By setting check, the scores of LTG in this study were evaluated. The germination rate at 15 degrees C for 10 d was scored to represent the LTG. The LTG of the RILs ranged from 0 to 99%. By single point analysis, simple interval mapping, and composite interval mapping, 5 putative QTL, qLTG-2, qLTG-6, qLTG-7, qLTG-LTG-11 and qLTG-12 were detected on chromosomes 2, 6, 7, 11 and 12 respectively. At the regions of qLTG-2, qLTG-6 and qLTG-11, DV85 alleles increased the LTG, while Kinmaze alleles increased it at the regions of qLTG-7 and qLTG-12. Among the five QTLs reported here, qLTG-2, qLTG-7 and qLTG-12 were newly detected, while the other two QTL-containing regions were close to those previously reported. Epistatic QTL were also detected in this paper.

Cold Temperature↗