PubMed Health⌕ Search

Biomedical subjects

Jian-hua Gao

Publications and source records attributed to Jian-hua Gao.

17 recordsLinked to original sources
PubMed2006

[Establishment of immortalized lymphoblastoid cell bank of a keloid pedigree].

OBJECTIVE: To investigate a method for establishing immortalized lymphoblastoid cell bank of keloid pedigree so as to provide a long-term source of specimens for keloid research. METHODS: With Epstein-Barr virus transformation, fresh and frozen blood samples collected from all members of the keloid pedigree were used respectively to establish the immortalized lymphoblastoid cell lines of B lymphocytes. RESULTS: Twenty-seven immortalized lymphoblastoid cell lines of the keloid pedigree were obtained successfully, and all cell lines survived cryopreservation in liquid nitrogen. CONCLUSIONS: The immortalized lymphoblastoid cell bank of the keloid pedigree can preserve the whole gene information and provide long-term DNA sources for keloid research. Preparation of the cell lines with fresh blood is more efficient than that with frozen blood.

Adolescent↗
PubMed2006

[Differential gene expression profile of keloids: a study with cDNA microarray].

OBJECTIVE: To investigate the differentially expressed genes in keloids in comparison with normal skin using cDNA microarray. METHODS: The cDNA microarray consisting of 8064 clones of human genes was employed to detect and screen the differentially expressed genes in keloid and normal skin tissues. Semi-quantitative RT-PCR was applied to verify the results of gene microarray. RESULTS: Totally 277 differentially expressed genes were identified in keloids in comparison with normal skin tissue, including 163 up-regulated genes and 114 down-regulated ones according to the designed data filter criteria. These differentially expressed genes belonged to 26 different functional gene families involving different biological processes. RT-PCR yielded results were consistent with those of microarray study. CONCLUSION: A variety of genes are involved in the formation of keloids. The 277 differentially expressed genes comprise the differential gene expression profile of keloids and describe the general changes in the gene expressions in keloid at transcriptional level. Further analysis of the identified genes might help reveal the molecular mechanism of abnormal scarring.

Connective Tissue Growth Factor↗
PubMed2006

[Linkage analysis of keloid susceptibility loci on chromosome 7p11 in a Chinese pedigree].

OBJECTIVE: To investigate whether the keloid susceptibility loci occur on chromosome 7p11 in a Chinese pedigree. METHODS: Peripheral venous blood samples from 32 members significant for hereditary investigation from a large Chinese keloid pedigree (with 5 affected generations) were collected for extracting genomic DNA. Four microsatellites on chromosome 7p11 with known maximal two-point LOD scores were selected as the genetic markers according to the method described in a recent report of the similar study. These markers were subsequently amplified by PCR, and all the PCR products were genotyped and linkage analyses were conducted. RESULTS: With the theta value of 0-0.1, the two-point LOD scores for these markers were all less than -2, which excluded the linkages of these markers to chromosome 7p11. CONCLUSION: This study provides the first genetic evidence that keloid susceptibility loci in Chinese pedigree do not locate on chromosome 7p11, suggesting the heterogeneity of familial keloid susceptibility loci.

Adolescent↗
PubMed2006

Adipose tissues differentiated by adipose-derived stem cells harvested from transgenic mice.

OBJECTIVE: To induce adipocyte differentiation in vitro by adipose-derived stromal cells (ASCs) harvested from transgenic mice with green fluorescent protein (GFP)and assess the possibility of constructing adipose tissues via attachment of ASCs to type I collagen scaffolds. METHODS: Inguinal fat pads from GFP transgenic mice were digested by enzymes for isolation of ASCs (primary culture). After expansion to three passages of ASCs, the cells were incubated in an adipogenic medium for two weeks, and the adipocyte differentiation by ASCs in vitro was assessed by morphological observation and Oil Red O staining. Then they were attached to collagen scaffolds and co-cultured for 12 hours, followed by hypodermic implantation to the dorsal skin of nude mice for 2 months. The newly-formed tissues were detected by HE staining. RESULTS: The cultured primary stem cells were fibroblast-like and showed active proliferation. After being incubated in an adipocyte differentiation medium, the lipid droplets in the cytoplasm accumulated gradually and finally developed into mature adipocytes, which showed positive in Oil Red O staining. A 0.5-cm3 new tissue clot was found under the dorsal skin of the nude mice and it was confirmed as mature adipose tissues by fluorescent observation and HE staining. CONCLUSIONS: ASCs can successfully differentiate adipose tissues into mature adipocytes, which exhibit an adipocyte-like morphology and express as intracytoplasmic lipid droplets. It is an efficient model of adipose tissues engineered with ASCs and type I collagen scaffolds.

Adipogenesis↗
PubMed2005

[A study of making use of computer-aided projection fringe system to measure breast dimension].

OBJECTIVE: To measure breast basic dimension by using computer-aided projection fringe system. METHODS: A system has been developed for measuring breast basic dimension based on computer-aided projection fringe measurement and programming software. Plastic manikins breast's SN-N (sternal notch to nipple distance), N-ML (nipple to midline distance), N-N (internipple distance), MBW (base width of breast) and N-IMF (nipple to inframammary fold distance) are measured with this system. At the same time, these items are also measured with routine ruler. RESULTS: This study indicate that the system has some merits: (1) non-touching measurement; (2) it is very rapid, the patient measured need hold his breath only 0.5 second, and all the time it takes is about 2.5 minutes; (3) the measurement's sensitivity is as high as to 0.6 mm, which meets the clinic requirement entirely; (4) the measurement's accuracy of the system is not significantly when comparing to the routine ruler's. CONCLUSIONS: Computer-aided projection fringe system for measuring breast basic dimension is feasible and advanced.

Breast↗
PubMed2005

[p53 gene codon 72 polymorphism and susceptibility to keloid].

OBJECTIVE: To investigate the relationship between p53 codon 72 polymorphism and susceptibility to keloid in a southern Chinese population. METHODS: The p53 genotypes were determined by polymerase chain reaction-reverse dot blot (PCR-RDB) and DNA direct sequencing in 45 patients with keloid and 60 unrelated healthy controls. RESULTS: The frequency of the p53 Pro allele among keloid patients was significantly higher than that among healthy controls (chi2 = 4.485, P = 0.034). The Pro/Arg and Arg/Arg genotype distribution among keloid patients was not significantly different from that among healthy controls (chi2 = 0.949, 1.346; P = 0.330, 0.246, respectively). However, the Pro/Pro genotype frequency among keloid patients was significantly higher than that among healthy controls (chi2 = 4.375, P = 0.036). The p53 Pro/Pro genotype significantly increased the risk for developing keloid, compared to the combination of Pro/Arg and Arg/Arg genotypes,with the odds ratio (OR) of 2.400 (95%CI: 1.048-5.498). CONCLUSIONS: Determination of the p53 codon 72 genotype may be used as a stratification marker to predicate high-risk individuals for keloid.

Adolescent↗
PubMed2005

[Analysizing and quantitatively evaluating the organic matter source at different ecologic zones of tidal salt marsh, North Jiangsu Province].

The indicators including TOC, TN, C/N, delta 13C and delta 15N of surface sediments, core sediments and plants of tidal salt marshes in North Jiangsu Province are analysized.Subsequently, distribution regularities of these measurement indicators are discussed, and the biogeochemistry processes between sediments and plants are also analysized. Lastly, the organic matter sources of different ecologic zones in tidal salt marsh are evaluated, and the organic matter accumulations in different ecologic zones induced by their plants are also compared. The results indicate that TOC, TN, C/N and delta 13C show obviously zonal distribution. The organic matter sources are dominated by marine input in silt flat, Artemisia schrenkiana flat, and the transition zone between silt and Spartina alterniflora flat, and controlled by terrigenous input in Spartina alterniflora flat. Spartina alterniflora plays an important role in the accumulation of organic matter in the whole tidal salt marshes ecosystem. In study area, the annually increased TOC, organic matter and TN in Spartina alterniflora flat, Artemisia schrenkiana flat and reed flat respectively reach 6 451t, 16 595t and 536t. The amount of TOC, organic matter and TN accumulated in Spartina alterniflora flat are more than those in other ecological zones, which show that the Spartina alterniflora flat exert nonreplaceable effect on the material cycle and exchange in the whole tidal salt marshes ecosystem.

Carbon Isotopes↗
PubMed2005

[A cross-sectional survey on hepatitis C virus infection among residents aged 18-59 years in a former commercial blood donating community, Shanxi Province].

OBJECTIVE: To determine sero-prevalence of and risk factors for hepatitis C virus infection (HCV) among residents aged 18-59 years in a former commercial blood donating community, Shanxi province. METHODS: A community-based cross-sectional survey was conducted among 660 randomly selected residents aged 18-59 years from 12 villages in a rural county of Shanxi province. Structured questionnaire was administered to collect socio-demographic,medical and risk behavioral information. 7 ml-volume venous blood was collected for HCV antibody testing. RESULTS: The overall HCV sero-prevalence rates were 8.2% and 27.7% among former commercial blood donors in the community. Data from multivariate logistic regression analysis showed that people ever selling blood (adjusted OR = 14.28, 95% CI: 6.83-29.87) and receiving blood transfusion (adjusted OR = 8.66,95% CI: 2.31-32.47) were the primary risk factors for HCV infection. Subgroup analysis in blood donors explored that ever selling plasma (adjusted OR = 8.56,95% CI: 2.87-25.54) was the risk factor for HCV infection. Villagers who stopped selling blood in 1994 and thereafter were less likely to be HCV positive than those stopping selling blood before 1994 (adjusted OR = 0.32,95% CI: 0.11-0.93). Female donors had a lower risk than male ones (adjusted OR = 0.28, 95% CI: 0.10-0.83). CONCLUSION: A HCV epidemic was once existing in rural community residents in the province that former commercial blood/plasma donation was the main reason for HCV epidemic in the community. It is urgent to make efficient measures to prevent HCV secondary transmission and provide patients with care and treatment to this community.

Adolescent↗
PubMed2004

[High efficient generation of recombinant adenovirus containing human Fas gene using a method of homologous recombination in bacteria].

OBJECTIVE: To generate recombinant adenovirus with human Fas gene and transfect the Fas gene into keloid-derived fibroblasts to take place of the dysfunctional Fas gene, reconstruct the blocked Fas signal. METHODS: We used a new bacterial homologous recombination system (Ad-Easy system) to construct recombinant adenovirus vector. Fas gene was cut down from the PMD-T-Fas plasmid and then transferred to track plasmid. Recombination was successfully completed in bacteria BJ5183 and recombinant adenovirus was produced in 293 cells. Then we infected keloid derived fibroblasts and compared the expression of Fas protein. Finally, we detected the function of newly produced Fas protein. RESULTS: We successfully constructed the recombinant adenovirus with Fas gene and detected its highly expressed Fas protein in infected keloid derived fibroblasts. Obvious apoptosis was also detected in infected keloid derived fibroblasts exposed to FasMcab. CONCLUSION: (1) The recombinant adenovirus with Fas gene can transfect the Fas gene into keloid-derived fibroblasts and highly improved the expression of Fas protein. The newly expressed Fas gene can reconstruct the blocked Fas signal. (2) The correlation between keloid and Fas gene was further proved and it may paves a sound foundation for further gene therapy in keloid.

Adenoviridae↗
PubMed2003

Immunohistochemical analysis of Fas protein expression in hypertrophic scar and keloid.

OBJECTIVE: To find an efficient diagnostic method of hypertrophic scar and keloid. METHODS: Immunohistochemistry was employed to detect the expression and distribution of Fas protein in keloids, hypertrophic scars and their surrounding normal skins. Image analysis was performed to quantitatively compare the differences of the protein expression in these tissues. RESULTS: The expression level of Fas antigen was much higher in the keloids and their surrounding normal skins than in hypertrophic scars, and no significant difference in the expression level between the former 2 tissues was found. In hypertrophic scars, however, much lower Fas protein expression was detected in comparison with that in their surrounding normal skins(P < 0.01). CONCLUSION: Immunohistochemical detection of Fas protein is a simple method that is well applicable in the differential diagnosis of pathological scars.

Cicatrix, Hypertrophic↗
PubMed2003

[Cell cycle analysis and P53 gene mutation detection of fibroblasts derived from the surrounding skin of keloids].

OBJECTIVE: To investigate whether there are abnormal fibroblasts in the surrounding skin of keloids for better understanding of the etiological feature of keloids. METHODS: Fresh samples were used for cell culture. Flow cytometry was used for analyzing cell cycles of fibroblasts derived from keloids and the surrounding skin. Proliferative cell proportions were compared between every two groups. P53 exon 4, 5 and 6 were amplified by PCR. DNA was sequenced to examine the structure of the destination gene. RESULTS: The proliferative cell proportion of the fibroblasts derived from the surrounding skin is not so high as that from the verge of keloids, but is higher than the normal skin fibroblasts derived from other part of the keloid patients or persons without keloids (P < 0.05). Mutations (point and frameshift mutations) of P53 exon 4 (6/6) and exon 5(2/6) in fibroblasts derived from the surrounding skin of keloids were identified. Fibroblasts derived from keloids have the same mutations as its surrounding skin. CONCLUSION: There are abnormal fibroblasts in the surrounding skin of keloids. It may be the consequence of keloid infiltrative growth and a reason of easy recurrence of keloid after therapy.

Cell Cycle↗
PubMed2003

[Prevention of the zygomatic branch of the facial nerve in rhytidectomy: an anatomical study].

OBJECTIVE: To investigate the anatomical distribution of the zygomatic branch of the facial nerve and discuss its clinical significance in the rhytidectomy. METHODS: The distribution of the zygomatic branch of the facial nerve was observed on 30 halves of the fifteen candaveric specimens (10 antiseptic cadaveric specimens and 5 fresh cadavers). RESULTS: The zygomatic branch made its way through the upper or the anterior border of the parotid gland, giving rise to 2 or 3 rami, which could be divided into the superior and the inferior rami. The superior rami, which were thin and superficial, crossed the zygomatic arch at its inner one-third or ran along the inferior margin of the zygomatic arch, and then entered beneath the zygomatic ligaments. The inferior rami were comparatively thick and deep, lying 1.0+/-0.3 cm inferior to the superior rami. The inferior and superior rami joined each other on the surface and deep side of the zygomatic major muscle. CONCLUSION: In the prevention of the zygomatic branch damage in rhytidectomy, the areas where caution should taken were the anterior border of the zygomatic major muscle in sub-SMAS dissection and the zygomatic arch in the subperiosteal dissection. The sharp dissection and excess tension should be avoided to reduce the nerve injury.

Adult↗
PubMed2003

[Microanatomy study of facial nerve distribution at the temporal region for safe facelifting].

OBJECTIVE: To investigate the route and scope of the facial nerve in the temporal region for clinical applications. METHODS: Temporal region dissection was performed on 12 cadavers (24 sides) under light microscope. RESULTS: There are two branches of the facial nerve in the temporal region from the superior margin of the parotid: the temporal branch and the zygomatic branch. Each of them has two to five branches, which run in the deep layer of the superficial temporal fascia. The temporal branch crosses the zygomatic arch to the temporal region, innervating the frontal muscle, the orbicularis oculi muscle, the corrugator supercilii muscle, and the muscle surrounding the ear, etc. The zygomatic branch goes to the lateral canthus, innervating the orbicularis oculi muscle, the upper and lower eyelid and zygomatic muscles. There are communicating branches among the temporal branches, the zygomatic branches and the supraorbital and lacrimal nerves of the ophthalmic nerve. CONCLUSION: The temporal branches and zygomatic branches of the facial nerve run between the deep zone of the superficial temporal fascia and the superficial layer of the profound temporal fascia, where dissection should be avoided during rhytidectomy in order not to damage the facial nerve branches.

Cadaver↗
PubMed2003

[Apoptosis of keloid-derived fibroblasts induced by Fas gene transfection].

OBJECTIVE: To observe the effect of Fas gene transfection on the apoptosis of keloid-derived fibroblasts. METHODS: The full-length cDNA of Fas was inserted into the multicloning site of the expression vector pcDNA-3.1 by molecular cloning technique, and the recombinant plasmid was transfected into the keloid-derived fibroblasts via lipofectin. Fas monoclonal antibody (mAb) was used to induce the apoptosis of the cells, which was evaluated with HE staining, DNA agarose gel electrophoresis and flow cytometry. RESULTS: The expression plasmid pcDNA-3.1-Fas was constructed successfully. Fas mAb induced apoptosis of these fibroblasts transfected with Fas gene, with the typical features of fibroblast apoptosis observed in the treated cells (e.g. typical apoptotic cell nuclei, DNA ladder and high apoptosis rate as determined by flow cytometer), but not in the control cells. CONCLUSION: Blockage of upstream apoptosis pathway in keloid-derived fibroblasts is due to nonfunction of Fas protein, and mutation of Fas gene may be the pathogenesis of keloids.

Apoptosis↗
PubMed2003

[Investigation of p53 gene mutations in keloids using PCR-SSCP].

OBJECTIVE: To detect gene mutations of p53 gene (exon 4-6) in fibroblasts. METHODS: Samples of keloids were taken from 15 patients. The mutations of p53 gene were detected using polymerase chain reaction, the single-strand conformational polymorphism(SSCP) analysis and DNA sequencing. RESULTS: Gene mutations in p53 gene exon 4, 5, and 6 were identified in all the patients with keloids. CONCLUSION: Gene mutations resulted in keloid p53 protein losing its functions of suppressing cell processes and conducting apoptosis.

Apoptosis↗
PubMed2002

[Charge transfer interaction of atropinum with sigma-electron acceptor and application research].

The charge transfer interaction of vitriol atropinum with iodine in the chloroform was studied. Iodine interacts with atropinum in the chloroform and forms the purple and brown charge transfer complexation. From the ultraviolet spectrum, it is confirmed that the maximum wavelength of the complexation is 280 nm and the apparent molar absorptivities of the complexation is 2.66 x 10(4) L.mol-1.cm-1. Using the equal mole consecutive change method, it is confirmed that the molar combining ratio is 1:1. The reaction mechanism is studied and it is confirmed that the complexation is a kind of supramolecule compound, which is in the syntonic mixed state of non-bonding structure and charge transfer. On the base of the complexation, the method that is used to determine vitriol atropinum injection by the charge transfer spectrophotometry is founded. And the optimal condition of the method was studied. The experiment showed that when the volume ratio of iodine and vitriol atropinum was 2:1, the value of the absorbency of the reaction system was almost stable and the reaction should last out for 20 minutes at room temperature. The method is simple and convenient, short-cut, exclusive. The linear range is 0-35 micrograms.mL-1, the reclaim rate is 99.6%, RSD is 0.83%.

Atropine↗