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Jianping Liu

Publications and source records attributed to Jianping Liu.

34 records · Page 2Linked to original sources
PubMed2003

[Genetic diversity analysis of the S10 gene of field and vaccine strains of Bluetongue virus].

S10 gene sequences of 1 attenuated vaccine strain and 31 Chinese field isolates & 1 South Africa strain of BTV were determined. The results revealed that all 33 S10 gene segments have 822 nucleotides in length with two in-frame initiation codons (nucleotides 20 to 22 and 59 to 61) and a common termination codon (nucleotides 707 to 709), which encodes two proteins (NS3 and NS3A). Nucleotide difference in the sequence of all S10 gene were from zero to 107 bp (86.4% - 100% identity). NS3/NS3A protein showed a light difference from zero to 10 amino acid (95.6% - 100% identity). Phylogenetic analysis of the S10 gene of above strains sequenced and 9 other strains from GenBank, segregated the Chinese viruses into a monophyletic group distinct from US viruses; Nucleotide identity was 85% between China Group and US group. The various Chinese isolates segregated into two phyletic subgroups based on S10 gene sequences. The clustering of viruses was dependent of geographical origin, and independent of host species of isolation, serotype & year of isolation.

Animals↗
PubMed2002

Lanthanide-transition-metal carbonyl complexes. 1. Syntheses and structures of ytterbium(II) solvent-separated ion pairs and isocarbonyl polymeric arrays of tetracarbonylcobaltate.

Transmetalation reactions of metallic ytterbium with Hg[Co(CO)(4)](2) in the coordinating solvents pyridine and THF yield the solvent-separated ion pairs [Yb(L)(6)] [Co(CO)(4)](2) (1a, L = Pyr; 2a, L = THF). The IR spectrum of 1a in pyridine indicates that the tetracarbonylcobaltate anion is not directly bonded to the divalent Yb cation owing to the strong coordinating ability of pyridine. On the other hand, IR spectra of 2a in THF are concentration dependent. In dilute solutions there is an equilibrium between the solvent-separated ion pair and a weak contact ion pair. Higher concentrations of 2a facilitate the formation of a tight ion pair that has a low-frequency isocarbonyl absorption. Remarkably, complexes 1a and 2a are easily transformed in toluene into the two-dimensional sheetlike arrays [(Pyr)(4)Yb[(mu-CO)(2)Co(CO)(2)](2)](infinity) (1b) and [(THF)(2)Yb[(mu-CO)(3)Co(CO)](2).Tol](infinity) (2b). The two-dimensional frameworks are supported by isocarbonyl linkages. Infrared spectra of toluene solutions substantiate the existence of the isocarbonyl bridges with low-frequency absorptions at 1780 cm(-1). Compounds 1b and 2b belong to a rare class of lanthanide-transition-metal carbonyl extended arrays, only three others of which have been structurally established. Dissolving 1b in pyridine regenerates 1a, but the complete conversion of 2b into 2a cannot be achieved. Crystal data: 1a.Pyr is monoclinic, P2(1)/c, a = 11.171(1) A, b = 11.925(1) A, c = 33.978(1) A, beta = 95.10(1) degrees, Z = 4; 2a is monoclinic, C2/c, a = 17.724(1) A, b = 12.468(1) A, c = 18.413(1) A, beta = 100.34(1) degrees, Z = 4; 1b is monoclinic, C2/c, a = 11.047(1) A, b = 13.423(1) A, c = 21.933(1) A, beta = 103.49(1) degrees, Z = 4; 2b is monoclinic, C2/c, a = 28.589(1) A, b = 7.223(1) A, c = 14.983(1) A, beta = 118.90(1) degrees, Z = 4.

Journal Article↗
PubMed2002

A draft sequence of the rice genome (Oryza sativa L. ssp. indica).

We have produced a draft sequence of the rice genome for the most widely cultivated subspecies in China, Oryza sativa L. ssp. indica, by whole-genome shotgun sequencing. The genome was 466 megabases in size, with an estimated 46,022 to 55,615 genes. Functional coverage in the assembled sequences was 92.0%. About 42.2% of the genome was in exact 20-nucleotide oligomer repeats, and most of the transposons were in the intergenic regions between genes. Although 80.6% of predicted Arabidopsis thaliana genes had a homolog in rice, only 49.4% of predicted rice genes had a homolog in A. thaliana. The large proportion of rice genes with no recognizable homologs is due to a gradient in the GC content of rice coding sequences.

Arabidopsis↗
PubMed2002

S-alkylation and S-amination of methyl thioethers--derivatives of closo-[B(12)H(12)](2-). synthesis of a boronated phosphonate, gem-bisphosphonates, and dodecaborane-ortho-carborane oligomers.

A variety of S-alkylated products was prepared by alkylation of methyl thioethers [MeSB(12)H(11)](2-) (5), [1-(MeS)-2(7,12)-(Me(2)S)B(12)H(10)](-) (6-8), and [1,2(7,12)-(MeS)(2)B(12)H(10)](2-) (9-11) with alkyl halides and tosylates in acetonitrile. Since these methyl thioethers can be prepared easily in B-10-enriched form on a large scale and due to their chemical versatility, they are potentially very attractive boron entities for the design and synthesis of therapeutics for boron neutron capture therapy of cancer. It was found that alkylation of 6-8 can be complicated by an equilibrium which establishes between, on the one hand, one of the former species and, on the other hand, 1,2(7,12)-(Me(2)S)(2)B(12)H(10) (2-4) and [1,2(7,12)-(MeS)(2)B(12)H(10)](2-) (9-11). A boronated phosphonate 1-(MeS(CH(2))(4)P(O)(OEt)(2))-7-(Me(2)S)B(12)H(10) (14g) and a gem-bisphosphonate 1-(MeS(CH(2))(3)CH[P(O)(OEt)(2)](2))-7-(Me(2)S)B(12)H(10) (14h) were prepared from thioether 7 and the corresponding iodide and tosylate, respectively, and subsequently converted to their sodium salts. The propargyl sulfonium salts obtained by alkylation of thioethers 7, 8, 10, and 11 with propargyl bromide have been further converted to two- and three-cage oligomers containing both ortho-carborane and dodecaborane moieties. Methyl thioethers derived from closo-[B(12)H(12)](2-) are excellent participants in Michael addition reactions in the presence of a strong acid. The sulfonium salts with tertiary alkyl and vinyl substituents have been prepared by this method. Methyl thioethers 5-11 react with hydroxylamine-O-sulfonate yielding the corresponding aminosulfonium salts, albeit in lower yields as compared to those in the alkylation reactions. Several derivatives of methyl thioethers 5-11 have been characterized by single-crystal X-ray diffraction.

Journal Article↗
PubMed2002

Artificial and bioartificial support systems for liver failure: a Cochrane Hepato-Biliary Group Protocol.

AIMS/BACKGROUND: Liver support systems may bridge patients to liver transplantation or recovery from liver failure. This review is to evaluate the beneficial and harmful effects of artificial and bioartificial support systems for acute and acute-on-chronic liver failure. DATA SOURCES: Randomized trials on any support system versus standard medical therapy will be included irrespective of publication status or language. Non-randomized studies are included in explorative analyses. Trials will be identified through bibliographies, correspondence with original investigators, and electronic searches (Cochrane Hepato-Biliary Group Controlled Trials Register, Cochrane Controlled Trials Register, MEDLINE, EMBASE, and The Chinese Biomedical Database). METHODS OF THE REVIEW: The extracted data will include characteristics of trials, patients, interventions, and all outcome measures. Methodological quality will be assessed by the randomization, follow up, and blinding. The RevMan and STATA will be used for statistical analyses. Sources of heterogeneity and methodological quality in the assessment of the primary outcome will be explored by sensitivity analyses and meta-regression.

Chronic Disease↗
PubMed2002

Misuse of randomization: a review of Chinese randomized trials of herbal medicines for chronic hepatitis B.

The quality of randomization of Chinese randomized trials on herbal medicines for hepatitis B was assessed. Search strategy and inclusion criteria were based on the published protocol. One hundred and seventy-six randomized clinical trials (RCTs) involving 20,452 patients with chronic hepatitis B virus (HBV) infection were identified that tested Chinese medicinal herbs. They were published in 49 Chinese journals. Only 10% (18/176) of the studies reported the method by which they randomized patients. Only two reported allocation concealment and were considered as adequate. Twenty percent (30/150) of the studies were imbalanced at the 0.05 level of probability for the two treatments and 13.3% (20/150) imbalanced at the 0.01 level in the randomization. It is suggested that there may exist misunderstanding of the concept and the misuse of randomization based on the review.

Data Interpretation, Statistical↗
PubMed2002

Role of angiotensin II and angiotensin II receptors in vascular smooth muscle cell migration in vitro.

OBJECTIVE: To determine the biotic effects of angiotensin II (Ang II) on the migration of rat smooth muscle cells (VSMCs) and investigate the mechanisms involved in the development of vascular injury. METHODS: VSMCs isolated from aortic media of Wistar rats and cultured by the modified explant method were adopted. In the presence and absence of Ang II, the expression of Ang II receptor (ATR) and reorganization of the actin cytoskeleton and focal adhesion of VSMCs were studied by an immunocytochemistry technique and fluorocytochemistry technique. Migration assays were performed with a modified Boyden's chamber. The effects of AT(1)R antagonist (CV-11974), AT(2)R antagonist (PD123319) on the aforementioned target were studied. RESULTS: VSMCs migration was stimulated by adding Ang II. The dynamic reorganization of actin cytoskeleton and focal adhesions may be an important mechanism by which Ang II facilitates VSMCs motility. The expression of AT(1)R in VSMCs could be upregulated initially after treatment with Ang II, then decreased gradually. The expression of AT(1)R was downregulated by AT(1)R antagonists. The effect of Ang II on VSMCs migration was mediated by AT(1)R, while AT(2)R had no significant effect. CONCLUSIONS: The dynamic reorganization of focal adhesions and the actin cytoskeleton is required for Ang II-induced VSMCs migration. This effect is mediated by AT(1)R.

Actins↗
PubMed1999

Cyclic Hydroborate Complexes of Metallocenes. IV. Weak Unsupported Single Hydrogen Bridges in Cp(2)Zr{(&mgr;-H)B(X)CH(2)Ph}{(&mgr;-H)(2)BX} (X = C(5)H(10), C(4)H(8)).

The compounds Cp(2)Zr{(&mgr;-H)(BC(5)H(10))CH(2)Ph}{(&mgr;-H)(2)BC(5)H(10)}, 1, and Cp(2)Zr{(&mgr;-H)(BC(4)H(8))CH(2)Ph}{(&mgr;-H)(2)BC(4)H(8)}, 2, were prepared in yields of 59% and 51%, respectively, from the reactions of Cp(2)ZrCl{(&mgr;-H)(2)BX} (X = C(5)H(10), C(4)H(8)) with PhCH(2)MgCl. Single-crystal X-ray diffraction analyses indicate the presence of an unsupported Zr-H-B bond in these complexes. In solution at low temperature (1)H NMR spectra are consistent with the presence of the unsupported Zr-H-B bridge in complexes 1 and 2. However, NMR spectra at room temperature indicate that the hydrogen bridge is dissociated into Cp(2)ZrH{(&mgr;-H)(2)BC(5)H(10)} and B(C(5)H(10))CH(2)Ph in the case of complex 1, and Cp(2)ZrH{(&mgr;-H)(2)BC(4)H(8)} and B(C(4)H(8))CH(2)Ph in the case of complex 2. It is possible to pump away the B(C(4)H(8))CH(2)Ph from solid 2 at room temperature, leaving behind Cp(2)ZrH{(&mgr;-H)(2)BC(4)H(8)}. These results suggest that the Zr-H bond acts as an electron pair donor to trivalent boron in the formation of 1and 2. Crystal data for Cp(2)Zr{(&mgr;-H)(BC(5)H(10))CH(2)Ph}{(&mgr;-H)(2)BC(5)H(10)}: monoclinic, P2(1)/m (No. 11), a = 9.392(5) Å, b = 13.250(4) Å, c = 9.841(6) Å, beta = 95.70(4) degrees, Z = 2. Crystal data for Cp(2)Zr{(&mgr;-H)(BC(4)H(8))CH(2)Ph}{(&mgr;-H)(2)BC(4)H(8)}: orthorhombic, Pbca (No. 61), a = 18.1630(10) Å, b = 19.0497(10) Å, c = 13.0393(10) Å, Z = 8.

Journal Article↗
PubMed1998

Heterometallic One-Dimensional Arrays Containing Cyanide-Bridged Lanthanide(III) and Transition Metals.

One-dimensional arrays having the general formula {(DMF)(10)Ln(2)[M(CN)(4)](3)}(infinity) [Ln = Sm, Eu, Er, Yb and M = Ni, Pd, Pt] were prepared from the reactions of 2:3 molar ratios of LnCl(3) with K(2)[M(CN)(4)] in DMF (DMF = N,N-dimethylformamide). Under similar conditions using 1:1 molar ratios of SmCl(3) and K(2)[Ni(CN)(4)] in DMF or YbCl(3) and K(2)[Ni(CN)(4)] in DMA (DMA = N,N-dimethylacetamide), the one-dimensional arrays {(DMF)(5)Sm[Ni(CN)(4)]Cl}(infinity), 8, and {(DMA)(4)Yb[Ni(CN)(4)]Cl}(infinity), 9, were prepared. An earlier study of {(DMF)(10)Yb[Ni(CN)(4)](3)}(infinity), 3, and {(DMF)(10)Yb(2)[Pt(CN)(4)](3)}(infinity), 7, showed that two different yet related one-dimensional arrays can be adopted. In the present study, X-ray crystal structures of {(DMF)(10)Sm(2)[Ni(CN)(4)](3)}(infinity), 1, and {(DMF)(10)Er(2)[Ni(CN)(4)](3)}(infinity), 2, are shown to be isomorphous with {(DMF)(10)Yb(2)[Ni(CN)(4)](3)}(infinity), 3, while {(DMF)(10)Sm(2)[Pd(CN)(4)](3)}(infinity), 4, {(DMF)(10)Eu(2)[Pd(CN)(4)](3)}(infinity), 5, and {(DMF)(10)Yb(2)[Pd(CN)(4)](3)}(infinity), 6, are isomorphous with {(DMF)(10)Yb(2)[Pt(CN)(4)](3)}(infinity), 7. Single-crystal X-ray crystal structure determinations reveal that arrays 1, 2, and 3 consist of cyanide-bridged "diamond"-shaped Ln(2)Ni(2) metal cores. These metal cores are linked together in an infinite array through cyanide bridges by [Ni(CN)(4)](2)(-) anions generating a single-strand chain. Crystal data for 1: triclinic space group P&onemacr;, a = 10.442(5) Å, b = 10.923(2) Å, c = 15.168(3) Å, alpha = 74.02(2) degrees, beta = 83.81(3) degrees, gamma = 82.91(2) degrees, Z = 2. Crystal data for 2: triclinic space group P&onemacr;, a = 10.172(1) Å, b = 11.111(3) Å, c = 15.369(2) Å, alpha = 73.17(2) degrees, beta = 85.15(1) degrees, gamma = 83.48(2) degrees, Z = 2. Arrays 4, 5, 6, and 7 consist of two parallel zigzag chains that are linked together through bridging [M(CN)(4)](2)(-) anions. Crystal data for 4: triclinic space group P&onemacr;, a = 9.304(2) Å, b = 11.351(3) Å, c = 16.257(5) Å, alpha = 81.62(2) degrees, beta = 77.51(2) degrees, gamma = 82.47(2) degrees, Z = 2. Crystal data for 5: triclinic space group P&onemacr;, a = 9.300(3) Å, b = 11.353(4) Å, c = 16.279(3) Å, alpha = 81.58(2) degrees, beta = 77.37(2) degrees, gamma = 81.58(2) degrees, Z = 2. Crystal data for 6: triclinic space group P&onemacr;, a = 9.164(2) Å, b = 11.718(3) Å, c = 16.122(3) Å, alpha = 79.88(2) degrees, beta = 74.43(2) degrees, gamma = 80.50(2) degrees, Z = 2. Electrical conductance, NMR, and infrared studies of DMF solutions of 1-7 reveal that these arrays are partially ionized in solution. Single-crystal X-ray analyses of the one-dimensional arrays 8 and 9 show that these complexes adopt the commonly observed zigzag chain structure. Crystal data for 8: monoclinic space group P2(1)/n, a =7.783(2) Å, b = 17.748(8) Å, c = 21.236(5) Å, beta = 92.87(2) degrees, Z = 4. Crystal data for 9: monoclinic space group P2(1)/n, a = 10.022(2) Å, b = 19.505(4) Å, c = 15.742(3) Å, beta = 105.94(2) degrees, Z = 4. Studies of 8 and 9 in DMF and DMA, respectively, indicate that 8 is partially ionized and 9 is almost completely ionized.

Journal Article↗
PubMed1998

Cyclic Hydroborate Complexes of Metallocenes II: Reactivity of (&mgr;-H)(2)(BC(5)H(10))(2) and Its Cyclic Derivative, [H(2)BC(5)H(10)](-); Synthesis of (eta(5)-C(5)H(5))(2)MCl(&mgr;-H)(2)BC(5)H(10) (M = Zr, Hf).

Reactions of the organodiborane (&mgr;-H)(2)(BC(5)H(10))(2), 1, with the Lewis bases, N(CH(3))(3), P(CH(3))(3), NH(3), and H(-), produced the cyclic adducts LHBC(5)H(10) (L = N(CH(3))(3), P(CH(3))(3), NH(3), H(-)) through symmetrical cleavage of the hydrogen bridge system. The salt [(NH(3))(2)BC(5)H(10)][H(2)BC(5)H(10)] was produced through unsymmetrical cleavage of the hydrogen bridge system. An improved, synthesis of the anion [H(2)BC(5)H(10)](-), 3, is described. It can function as a hydride transfer reducing agent in its reactions with BH(3)THF and 4-tert-butylcyclohexanone. In its reactions with (eta(5)-C(5)H(5))(2)MCl(2) it serves as a chelating agent to produce (eta(5)-C(5)H(5))(2)MCl(&mgr;-H)(2)BC(5)H(10) (M = Zr, 4; Hf, 5). The molecular structures of 1 and 4 are reported here. Crystal data for 1: space group P&onemacr;, a = 6.415(3) Å, b = 9.260(4) Å, c =10.291(5) Å, alpha = 114.53(4) degrees, beta = 104.25(4) degrees, gamma = 90.01(4) degrees, Z = 2. Crystal data for 4: space group P2(1)/c, a = 12.584(2) Å, b = 9.511(1) Å, c = 12.813(2) Å, beta = 100.26(1) degrees, Z = 4.

Journal Article↗
PubMed1997

Hydroboration of (&mgr;-H)(2)Os(3)(CO)(10): Formation of the Borylidyne Cluster (&mgr;-H)(2)Os(3)(CO)(9)(&mgr;-H)(2)BH. An Improved Synthesis of (&mgr;-H)(3)Os(3)(CO)(9)(&mgr;(3)-BCO).

At room temperature, the reaction of (&mgr;-H)(2)Os(3)(CO)(10) with BH(3)S(CH(3))(2) produces the new borylidyne cluster (&mgr;-H)(2)Os(3)(CO)(9)(&mgr;-H)(2)BH, a methylidyne cluster analog. At 65 degrees C the previously reported ketenylidene cluster analog (&mgr;-H)(3)Os(3)(CO)(9)(&mgr;(3)-BCO) is formed. The complex (&mgr;-H)(2)Os(3)(CO)(9)(&mgr;-H)(2)BH was characterized by (1)H NMR, (11)B NMR, and infrared spectroscopies as well as mass spectrometry. Its molecular structure was determined from a single-crystal X-ray analysis. The molecule is of approximate C(s)() symmetry. It consists of a tetrahedral Os(3)B core with a terminal B-H bond, two Os-H-B, hydrogen bridge bonds, and three Os(CO)(3) units. A similar structure has been proposed for one of the isomers of a ruthenium analog, (&mgr;-H)(2)Ru(3)(CO)(9)(&mgr;-H)(2)BH, but it differs from the related iron complex (&mgr;-H)Fe(3)(CO)(9)(&mgr;-H)(3)BH which has three Fe-H-B bridge bonds. Crystal data for (&mgr;-H)(2)Os(3)(CO)(9)(&mgr;-H)(2)BH: triclinic space group P&onemacr;, a = 9.501(4) Å, b = 9.525(4) Å, c = 10.070(2) Å, alpha = 87.72(3) beta = 88.32(3) degrees, gamma = 61.45(3) degrees, Z = 2.

Journal Article↗
PubMedDate not supplied

Molecular cloning and characterization of a novel human kinase gene, PDIK1L.

We isolated a 4301-bp cDNA from a human foetal brain cDNA library by high-throughput cDNA sequencing. It encodes a protein of 341 amino acids, which shows 69% identity with the human kinase CLIK1 (AAL99353), which was suggested to be the CLP-36 interacting kinase. Bioinformatics analysis suggests that the putative kinase may interact with PDZ and LIM domain proteins. Therefore the protein and its cDNA were named 'PDLIM1 interacting kinase 1 like' (PDIK1L; nomenclature approved by the HUGO Gene Nomenclature Committee). Ensembl Genome Browser located PDIK1L to human chromosome 1p35.3. It spans about 13.7 kb and consists of four exons and three introns. Multiple-tissue cDNA panel PCR revealed that the gene is expressed widely in human tissues: liver, kidney, pancreas, spleen, thymus and prostate. The protein appears to be localized to the nucleus.

Amino Acid Sequence↗