Biomedical subjects

Jiaxin Yu

Publications and source records attributed to Jiaxin Yu.

3 recordsLinked to original sources

PubMed2026

Plasma untargeted metabolomics reveals promising diagnostic metabolites for adrenocortical carcinoma.

Adrenocortical carcinoma (ACC) is a rare and aggressive malignancy with poor prognosis. Surgery is the only curative option for ACC, but recurrence remains high. Effective systemic therapies are still lacking in ACC. Diagnosis currently relies on integrated hormonal, imaging, and histopathological assessments. However, distinguishing ACC from benign adrenocortical adenoma (ACA) remains challenging. To address this, we performed untargeted plasma metabolomics on patients with ACC and ACA, as well as on healthy controls, to identify differential metabolites and elucidate the enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Notably, glycocholic acid emerged as a significantly upregulated metabolite in ACC with superior diagnostic accuracy. Furthermore, a metabolic model incorporating six plasma metabolites, including glycocholic acid, distinguished ACC from ACA and healthy controls with an area under the curve (AUC) of 0.9266. These findings demonstrate that plasma metabolomics might serve as a promising tool for the detection of ACC.

adrenocortical carcinoma↗

PubMed2002

Cocaine detoxification by combinatorially substituted beta-cyclodextrin libraries.

Per-6-substituted- and A,C,E(F)-tri-6-substituted-6-deoxy-beta-cyclodextrin (beta-CD) libraries were generated using solution-phase combinatorial chemistry techniques starting from the corresponding iodo precursors and different combinations of individual amine nucleophiles. Using a high throughput electrospray mass spectrometry (ESMS) screen to monitor the hydrolysis of cocaine, certain libraries showed the ability to specifically hydrolyze the methyl ester of cocaine, with the most active per-6-substituted beta-CD library I producing complete hydrolysis in 24h. The cocaine hydrolytic activity in this series showed structure-activity relationships which appeared to involve specific interaction between the amine side chains and the cocaine molecule. Comparison of the composition of the most active per-6-substiuted beta-CD libraries and A,C,E(F)-tri-6-substituted-6-deoxy-beta-CD libraries (I and XV) showed three common side chains (3, 4, and 5), suggesting that active side chains in the tri-substituted beta-CD library might be predicted from evaluation of the more easily prepared per-6-substituted-6-deoxy-beta-CD series.

Cocaine↗

PubMedDate not supplied

Per-6-substituted beta-cyclodextrin libraries inhibit formation of beta-amyloid-peptide (A beta)-derived, soluble oligomers.

Alzheimer's disease is the most common cause of dementia in older individuals with compelling evidence favoring neuron dysfunction and death triggered by assembled forms of A beta(1-42). While large neurotoxic amyloid fibrils have been known for years, recent studies show that soluble protofibril and A beta(1-42)-derived diffusible ligands (ADDLs) may also be involved in neurotoxicity. In the present work, dot-blot immunoassays discriminating ADDLs from monomers were used to screen libraries of per-substituted beta-cyclodextrin (beta-CD) derivatives for inhibition of ADDLs formation. Libraries were prepared from per-6-iodo-beta-CD by treatment with various amine nucleophiles. The most active library tested (containing >2000 derivatives) was derived from imidazole, N, N-dimethylethylenediamine and furfurylamine, which at 10 microM total library, inhibited ADDLs formation (10 nM A beta(1-42)) over a period of 4 hours. The latter was confirmed by a western blot assay showing decreased amounts of the initially formed A beta(1-42) tetramer. These preliminary experiments suggest that derivatized forms of beta-CD can interfere with the oligomerization process of A beta(1-42).

Alzheimer Disease↗