Biomedical subjects

Jie Cui

Publications and source records attributed to Jie Cui.

12 recordsLinked to original sources

PubMed2006

Reduced cerebral ischemia-reperfusion injury in Toll-like receptor 4 deficient mice.

Inflammatory reaction plays an important role in cerebral ischemia-reperfusion injury, however, its mechanism is still unclear. Our study aims to explore the function of Toll-like receptor 4 (TLR4) in the process of cerebral ischemia-reperfusion. We made middle cerebral artery ischemia-reperfusion model in mice with line embolism method. Compared with C3H/OuJ mice, scores of cerebral water content, cerebral infarct size and neurologic impairment in C3H/Hej mice were obviously lower after 6 h ischemia and 24 h reperfusion. Light microscopic and electron microscopic results showed that cerebral ischemia-reperfusion injury in C3H/Hej mice was less serious than that in C3H/OuJ mice. TNF-alpha and IL-6 contents in C3H/HeJ mice were obviously lower than that in C3H/OuJ mice with ELISA. The results showed that TLR4 participates in the process of cerebral ischemia-reperfusion injury probably through decrease of inflammatory cytokines. TLR4 may become a new target for prevention of cerebral ischemia-reperfusion injury. Our study suggests that TLR4 is one of the mechanisms of cerebral ischemia-reperfusion injury besides its important role in innate immunity.

Animals↗

PubMed2006

Automated search for arthritic patterns in infrared spectra of synovial fluid using adaptive wavelets and fuzzy C-means analysis.

Analysis of synovial fluid by infrared (IR) clinical chemistry requires expert interpretation and is susceptible to subjective error. The application of automated pattern recognition (APR) may enhance the utility of IR analysis. Here, we describe an APR method based on the fuzzy C-means cluster adaptive wavelet (FCMC-AW) algorithm, which consists of two parts: one is a FCMC using the features from an M-band feature extractor adopting the adaptive wavelet algorithm and the second is a Bayesian classifier using the membership matrix generated by the FCMC. A FCMC-cross-validated quadratic probability measure (FCMC-CVQPM) criterion is used under the assumption that the class probability density is equal to the value of the membership matrix. Therefore, both values of posterior probabilities and selection criterion MFQ can be obtained through the membership matrix. The distinctive advantage of this method is that it provides not only the 'hard' classification of a new pattern, but also the confidence of this classification, which is reflected by the membership matrix.

Algorithms↗

PubMed2006

The adaptive chirplet transform and visual evoked potentials.

We propose a new approach based upon the adaptive chirplet transform (ACT) to characterize the time-dependent behavior of the visual evoked potential (VEP) from its initial transient portion (tVEP) to the steady-state portion (ssVEP). This approach employs a matching pursuit (MP) algorithm to estimate the chirplets and then a maximum-likelihood estimation (MLE) algorithm to refine the results. The ACT decomposes signals into Gaussian chirplet basis functions with four adjustable parameters, i.e., time-spread, chirp rate, time-center and frequency-center. In this paper, we show how these four parameters can be used to distinguish between the transient and the steady-state phase of the response. We also show that as few as three chirplets are required to represent a VEP response. Compared to decomposition with Gabor logons, a more compact representation can be achieved by using Gaussian chirplets. Finally, we argue that the adaptive chirplet spectrogram gives a superior visualization of VEP signals' time-frequency structures when compared to the conventional spectrogram.

Algorithms↗

PubMed2006

[Surgical management of neonate lip and palate cleft].

OBJECTIVE: To explore the security and feasibility in ambi-operation of the neonate lip and palate cleft correction. METHODS: Using Ornizuka's and innovated Langenbeck's method to correct the lip and palate cleft in 30 cases with detailed program, the effect after operation was evaluated. RESULTS: All physiological index were normal in the group of ambi-operation, which had no difference to other newborn. The effect is satisfactory. 5 of them have the fistula under the gingival. CONCLUSIONS: Based on controlling on surgery indication, screening the well-developed newborn, it is secure and feasible to correct lip and palate in one stage, and it can get excellent effect.

Cleft Lip↗

PubMed2006

[Total calvarial reconstruction for sagittal synostosis].

OBJECTIVE: To study the technique of total calvaria reconstruction for sagittal synostosis. METHODS: 11 children with sagittal synostosis were treated. The David II procedure was used to reconstruct the calvarial gap in 6 cases; Osteotomies with reversal change was performed on 2 cases; Plum-plasty of the calvaria was performed on 3 cases. RESULTS: The 11 cases were cured with satisfactory head shape. CONCLUSIONS: The David II procedure is applicable for patient of 1 approximately 3 months. The technique of floating skull flap plasty and plum-plasty of the calvaria can be performed on patients older than 3 months.

Cranial Sutures↗

PubMed2005

[Analysis of the methylation in the promoter of LRP15 gene and its expression].

To study the methylation in the promoter of LRP15 gene and its relationship with gene expression and to explore the possible mechanism of regulating LRP15 gene methylation, the methylation in the promoter of LRP15 gene in K562 cell line was detected by MS-PCR. Then K562 was exposed to 5-aza-2'-deoxycytidine (CdR) and trichostatin (TSA), to determine whether the silencing of LRP15 gene by de novo methylation could be reversed. As a result, it was confirmed by MS-PCR that the promoter of LRP15 was hypermathylated in K562 cell line, and lost its transcription activity. After CdR, with or without TSA, the silencing of LRP15 gene by de novo methylation can be reversed. Observation demonstrated that the expression of LRP15 was controlled by methylation in its promoter in K562. It is suggested that methyltransferase inhibitor and deacetylase inhibitor may be effective agents in leukemia therapy.

Azacitidine↗

PubMed2004

Regulation of the type 1 inositol 1,4,5-trisphosphate receptor by phosphorylation at tyrosine 353.

The inositol 1,4,5-trisphosphate receptor (IP3R) plays an essential role in Ca2+ signaling during lymphocyte activation. Engagement of the T cell or B cell receptor by antigen initiates a signal transduction cascade that leads to tyrosine phosphorylation of IP3R by Src family nonreceptor protein tyrosine kinases, including Fyn. However, the effect of tyrosine phosphorylation on the IP3R and subsequent Ca2+ release is poorly understood. We have identified tyrosine 353 (Tyr353) in the IP3-binding domain of type 1 IP3R (IP3R1) as a phosphorylation site for Fyn both in vitro and in vivo. We have developed a phosphoepitope-specific antibody and shown that IP3R1-Y353 becomes phosphorylated during T cell and B cell activation. Furthermore, tyrosine phosphorylation of IP3R1 increased IP3 binding at low IP3 concentrations (<10 nm). Using wild-type IP3R1 or an IP3R1-Y353F mutant that cannot be tyrosine phosphorylated at Tyr353 or expressed in IP3R-deficient DT40 B cells, we demonstrated that tyrosine phosphorylation of Tyr353 permits prolonged intracellular Ca2+ release during B cell activation. Taken together, these data suggest that one function of tyrosine phosphorylation of IP3R1-Y353 is to enhance Ca2+ signaling in lymphocytes by increasing the sensitivity of IP3R1 to activation by low levels of IP3.

Binding Sites↗

PubMed2004

Noise exposure of music teachers.

A noise exposure survey was performed to assess the risk of hearing loss to school music teachers during the course of their activities. Noise exposure of 18 teachers from 15 schools was measured using noise dosimeters. The equivalent continuous noise level (L(eq)) of each teacher was recorded during single activities (classes) as well as for the entire day, and a normalized 8-hour exposure, termed the noise exposure level (L(ex)) was also computed. The measured L(eq) exceeded the 85-dBA limit for 78% of the teachers. L(ex) exceeded 85 dBA for 39% of the teachers. Limited recommendations on how to reduce the noise exposures are provided. The need for a hearing conservation program has also been emphasized.

Environmental Monitoring↗

PubMed2004

S641 contributes HERG K+ channel inactivation.

The kinetics of voltage-dependent inactivation of the rapidly activating delayed rectifier, IKr, are unique among K+ channels. The human ether-a-gogo-related gene (HERG) encodes the pore-forming subunit of IKr and shares a high degree of homology with ether-a-gogo (EAG) channels that do not inactivate. Within those segments thought to contribute to the channel pore, HERG possesses several serine residues that are not present in EAG channels. Two of these serines, S620 and S631, are known to be required for inactivation. We now show that a third serine, S641, which resides in the outer portion of the sixth transmembrane segment, is also critical for normal inactivation. As with the other serines, S641 is also involved in maintaining ion selectivity of the HERG channel and alters sensitivity to block by E4031. Larger charged or polar substitutions (S641D and S641T) disrupted C-type inactivation in HERG. Smaller aliphatic and more conservative substitutions (S641A and S641C) facilitated C-type inactivation. Our data show that, like S620 and S631, S641 is another key residue for the rapid inactivation. The altered inactivation of mutations at S620, S631, and S641 were dominant, suggesting that a network of hydroxyl side chains is required for the unique inactivation, permeation, and rectification of HERG channels.

Amino Acid Sequence↗

PubMed2002

CaT1 contributes to the stores-operated calcium current in Jurkat T-lymphocytes.

T-lymphocyte activation requires sustained Ca(2+) signaling dependent upon capacitative Ca(2+) entry (CCE). The protein(s) that forms the stores-operated Ca(2+) channel (SOCC) responsible for CCE has long been sought but has not been definitively identified. Members of the TRPV family (transient receptor potential superfamily-vanilloid receptor subfamily) of channel genes have been proposed to encode SOCCs responsible for CCE in non-excitable cells. Here we present evidence that a member of the TRPV group, CaT1, is involved in generating I(CRAC), the CCE current that is necessary for T-cell activation. CaT1 is expressed in Jurkat T-lymphocytes. When overexpressed in Jurkat cells, CaT1 produces a Ca(2+) entry current that mimics the endogenous I(CRAC) in its dependence on external Ca(2+), inactivation by elevated concentration of internal Ca(2+), and pharmacological block by capsaicin. Overexpressed CaT1 is partially regulated by the release of internal Ca(2+) stores via thapsigargin or receptor-mediated generation of inositol 1,4,5-trisphosphate. A pore-region mutant of CaT1, TRIA-CaT1, fails to carry Ca(2+) currents and associates with co-expressed wild type CaT1 to functionally suppress permeation of Ca(2+) ions. Expression of the TRIA-CaT1 mutant in Jurkat cells results in suppression of the endogenous I(CRAC). Taken together these results indicate that CaT1 is the channel protein that contributes to T-lymphocyte SOCCs either alone or as a subunit in a heterogeneous channel complex.

Animals↗

PubMed2002

The effects of exposure to a 1.5-tesla magnetic field on intravitreous metallic foreign bodies in rabbits.

BACKGROUND: The study was performed to determine (1) whether intravitreous ferromagnetic foreign bodies (FBs) are sufficiently mobile in a magnetic field to induce acute injury in vivo, and (2) whether the length of time from implantation of the intravitreous FB affects mobility. METHODS: A 3 mm x 0.72 mm magnetic FB (MFB) and a non-magnetic metallic FB (NMFB) of similar dimensions were surgically implanted into the right and left eyes, respectively, of 20 New Zealand white rabbits. On days 0 (FB implantation) and 30, all rabbits underwent computed tomography (CT) prior to and immediately following exposure to a 1.5-tesla (T) magnetic field. Pre- and post-magnetic field CT image FB angles and distances, together with ophthalmic examinations, were used to assess FB mobility. Rabbits were killed at 31 days. The globes were processed for light microscopy (LM) and scanning electron microscopy (SEM). RESULTS: Angle measurements revealed that the MFBs developed significant angular motion and were scattered more widely than NMFBs on the day of FB implantation and 30 days later, although the angular motion and scatter of MFBs were less on day 30. There were no significant differences among distance measurements for MFBs or NMFBs on or between days 0 and 30. Throughout the 30-day study, ophthalmic examinations revealed dorso-ventral FB movement and focal vitreous degeneration and retinal hemorrhage within both MFB- and NMFB-containing globes. Mild vitreous hemorrhage was noted around a NMFB. Focal retinal degeneration was confirmed by LM ( n=1 MFB). SEM revealed intravitreous erythrocytes and spindle cells adjacent to all FBs examined. CONCLUSIONS: (1) Angle measurements show that MFBs are significantly mobile when exposed to a 1.5-T magnetic field on the same day as FB implantation and 30 days later. (2) Angular mobility of MFBs is less at 30 days. (3) Magnetic field exposure did not induce ocular injury in MFB-containing globes. (4) Intravitreous metallic FB retention induces ocular pathologic changes.

Animals↗

PubMedDate not supplied

Reliability analysis of DOOF for Weibull distribution.

Hierarchical Bayesian method for estimating the failure probability p(i) under DOOF by taking the quasi-Beta distribution B(p(i-1), 1, 1, b) as the prior distribution is proposed in this paper. The weighted Least Squares Estimate method was used to obtain the formula for computing reliability distribution parameters and estimating the reliability characteristic values under DOOF. Taking one type of aerospace electrical connector as an example, the correctness of the above method through statistical analysis of electrical connector accelerated life test data was verified.

Equipment Failure↗