Biomedical subjects

Jin Su

Publications and source records attributed to Jin Su.

22 records · Page 2Linked to original sources

PubMed2002

Emergency management of respiratory failure by endotracheal intubation through the nose under the guidance of portable bedside fibrobronchoscope.

OBJECTIVE: To investigate the value of portable fibrobronchoscope in the management of respiratory failure by endotracheal intubation through the nose. METHODS: Fifty-eight patients with acute or chronic respiratory failure received mechanical ventilation by endotracheal intubation through the nose under the guidance of portable fibrobronchoscope. RESULTS: Intubation was successfully performed in all the patients in a single attempt (which took 30 min to 5 min) without interruption of autonomous breathing or incurring laryngospasm or cardiac arrest. After mechanical ventilation for 30 min, conspicuous improvement of respiratory failure was observed in all the cases. CONCLUSION: With convenient and easy manipulation, portable fibrobronchoscope provides quick and accurate guidance for endotracheal intubation through the nose in the emergency management of respiratory failure.

Adult↗

PubMed2002

Inhibitive effects of glucose and free fatty acids on proliferation of human vascular endothelial cells in vitro.

OBJECTIVES: To investigate the effects of glucose and free fatty acids (FFAs) on the proliferation and cell cycle of human vascular endothelial cells in vitro, and to examine whether the combined presence of elevated FFAs and glucose may cross-amplify their individual injurious effects. METHODS: Cultured human vascular endothelial cells (ECV304) were incubated with various concentrations of glucose and/or FFAs (palmitate and/or oleate) for 24 - 96 h. Morphologic alterations were observed using a phase contrast microscope and an electron microscope. Inhibition of proliferation was measured by a colorimetric 3-[4, 5-dimethyl thiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) assay. Cell viability was determined using trypan blue exclusion. Distribution of cells along phases of the cell cycle was analyzed by flow cytometry. RESULTS: Glucose 15 or 30 mmol/L, palmitate (PA) 0.25 or 0.5 mmol/L, and oleate (OA) 0.5 mmol/L inhibited proliferation and accelerated death of endothelial cells in a dose-and-time-dependent manner. After treatment with elevated glucose and/or FFAs, the G(0)/G(1) phase cells increased, whereas S phase cells decreased, suggesting that high glucose and/or FFAs mainly arrested endothelial cells at G(0)/G(1) phase. The inhibitive rates of proliferation and population of dead cells in endothelial cells incubated with glucose plus FFAs (glucose 30 mmol/L + PA 0.25 mmol/L, glucose 30 mmol/L + OA 0.5 mmol/L, glucose 30 mmol/L + PA 0.25 mmol/L + OA 0.5 mmol/L) increased more markedly than those treated with high glucose or FFAs (PA and/or OA) alone. CONCLUSION: Both high ambient glucose and FFAs can inhibit proliferation and accelerate death of endothelial cells in vitro. These changes were cross-amplified in the combined presence of high levels of glucose and FFAs.

Cell Division↗

PubMed2001

Preparation of anfi-CD3 and anti-IgM&mgr; chain bispecific antibody by cell fusion.

OBJECTIVE: To prepare anti-CD3 and anti-Igm&mgr; chain bispecific antibody (BsAb) by means of cell fusion and assess the stability and activity of the hybrid hybridoma. METHODS: Mouse hybridoma cell line secreting anti-human CD3 monoclonal antibody (mAb) was trans formed into HAT-sensitive cells by 8-azaguanine, which was subsequently transfected by plasmid pCDaA3 containing neoR marker gene via FuGENETM6. The resulted mutant phenotype, named alphaCD3 HATs G418R, was fused with the hybridoma producing anti-IgM&mgr; chain mAb, and enzyme-linked immunosorbent assay and flow cytometry were employed to identify the fusion cells producing the target BsAb. RESULTS: Six rounds of cell fusion was performed and a total of l 080 wells inoculated, yielding 5 hybrid hybridoma cell lines. Two of the 5 cell lines were subcloned and continuously cultured in vitro for 2 months without losing their capability to secrete BsAb. CONCLUSION: Cell fusion technique can be utilized to prepare BsAb-producing hybrid hybridoma that has similar stability and activity to its parent hybridoma.

Journal Article↗

PubMedDate not supplied

Omega-3 fatty acids and selenium as coronary heart disease risk modifying factors in Asian Indian and Chinese males.

OBJECTIVE: Asian Indian men are reported to have a higher incidence of coronary heart disease than men of other ethnic groups worldwide. Among the many hypotheses, one possible risk factor may be related to their dietary habits. This study estimated the plasma concentrations of fatty acids, antioxidant vitamins, and selenium in Indians and Chinese of Singapore. METHODS: The study population consisted of 145 Indian men and 147 Chinese men ages 26 to 79 y from a cross-sectional survey, the National University of Singapore Heart Study. RESULTS: Our findings indicated that Indians had lower plasma concentrations of docosahexanoic acid (3.07% versus 3.54%, P < 0.001), alpha-linolenic acid (0.48% versus 0.57%, P < 0.001), and total omega-3 fatty acids (4.71% versus 5.27%, P < 0.001) than did the Chinese. Arachidonic acid was higher in Indians (4.83%) than in the Chinese (4.51%, P = 0.007). The ratio of omega-3 acid to omega-6 fatty acid was also lower in Indians (0.15) than in the Chinese (0.16, P = 0.007). There were no significant differences in the concentrations of monounsaturated fatty acids, but saturated fatty acids were higher in Indians (39.17%) than in the Chinese (38.28%, P < 0.001). Analysis of vitamins A, C, and E showed no significant differences between Indians (0.67, 5.72, and 13.04 mg/L, respectively) and Chinese (0.68, 6.48, and 12.71 mg/L, respectively); however, serum concentration of selenium in Indians (117.49 microg/L) was significantly lower than in the Chinese (126.72 microg/L, P < 0.001). CONCLUSION: The results suggest that lower plasma concentrations of omega-3 fatty acids and selenium and higher concentrations of arachidonic acid and saturated fatty acids in Indians may reflect lower intakes of marine foods and, as a consequence, higher susceptibility to coronary heart disease.

Adult↗