Biomedical subjects

Jin-Ling Meng

Publications and source records attributed to Jin-Ling Meng.

4 recordsLinked to original sources

PubMed2006

QTL mapping of seed coat color for yellow seeded Brassica napus.

The development of yellow-seeded varieties of Brassica napus for improving the oilseed quality characteristics of lower fiber content and higher protein and oil content has been a major focus of breeding researches worldwide in recent years. With the black-seeded 'Youyan 2' as male and the yellow-seeded GH06 as female parents respectively, F2 population of 132 individuals were obtained. A linkage map was constructed with 164 markers including 125 AFLP, 37 SSR, 1 RAPD and 1 SCAR markers distributed over 19 linkage groups covering approximately 2 549.8 cM with an average spacing of 15.55 cM. Two loci located on the 5th and 19th group were detected for the trait of seed coat color based on the linkage group using multiple interval mapping method and explained 46% and 30.9% of the phenotypic variation, respectively.

Amplified Fragment Length Polymorphism Analysis↗

PubMed2006

RFLP and AFLP analysis of inter- and intraspecific variation of Brassica rapa and B. napus shows that B. rapa is an important genetic resource for B. napus improvement.

Fingerprinting of 29 accessions of oilseed rape, including seven accessions of Brassica napus, and 22 accessions of B. rapa (B. campestris) from Europe, North America, and China was analyzed using RFLP and AFLP markers. In total, 1,477 polymorphic RFLP bands and 183 polymorphic AFLP bands from 166 enzyme-probe combinations and two pairs of AFLP primers, respectively, were scored for the 29 accessions. On average, RFLP analysis showed that the Arabidopsis EST probe detected more polymorphic bands in Brassica than the random genomic probe performed. More polymorphic RFLP markers were detected with the digestion of EcoR I or BamH I than HindIII. According to the number of bands amplified from each accession, the copy numbers of each gene in the genomes of B. rapa and B. napus were estimated. The average copy numbers in B. rapa of China, B. rapa of Europe, and B. napus, were 3.2, 3.1, and 2.9, respectively. Genetic distance based on the AFLP data was well correlated with that based on the RFLP data (r = 0.72, P<0.001), but 0.39 smaller on average. Genetic diversity analysis showed that Chinese B. rapa was more polymorphic than Chinese B. napus and European materials. Some European B. napus accessions were clustered into European B. rapa, which were distinctly different from Chinese B. napus. The larger variations of Chinese accessions of B. rapa suggest that they are valuable in oilseed rape breeding. Novel strategies to use intersubgenomic heterosis between genome of B. rapa (A(r)A(r)) and genome of B. napus (A(n)A(n)C(n)C(n)) were elucidated.

Amplified Fragment Length Polymorphism Analysis↗

PubMed2003

[MapDraw: a microsoft excel macro for drawing genetic linkage maps based on given genetic linkage data].

MAPMAKER is one of the most widely used computer software package for constructing genetic linkage maps.However, the PC version, MAPMAKER 3.0 for PC, could not draw the genetic linkage maps that its Macintosh version, MAPMAKER 3.0 for Macintosh,was able to do. Especially in recent years, Macintosh computer is much less popular than PC. Most of the geneticists use PC to analyze their genetic linkage data. So a new computer software to draw the same genetic linkage maps on PC as the MAPMAKER for Macintosh to do on Macintosh has been crying for. Microsoft Excel,one component of Microsoft Office package, is one of the most popular software in laboratory data processing. Microsoft Visual Basic for Applications (VBA) is one of the most powerful functions of Microsoft Excel. Using this program language, we can take creative control of Excel, including genetic linkage map construction, automatic data processing and more. In this paper, a Microsoft Excel macro called MapDraw is constructed to draw genetic linkage maps on PC computer based on given genetic linkage data. Use this software,you can freely construct beautiful genetic linkage map in Excel and freely edit and copy it to Word or other application. This software is just an Excel format file. You can freely copy it from ftp://211.69.140.177 or ftp://brassica.hzau.edu.cn and the source code can be found in Excel's Visual Basic Editor.

English Abstract↗

PubMed2002

[Integration of DNA clones related to important economic traits of Brassica napus onto Arabidopsis genetic map].

Arbidopsis thaliana and Brassica oilcrops are closely related species in Brassicae, Cruciferae, and show highly homologous genomes. After a serial important agronomic characteristics of Brassica napus being mapped with probes from EST clones of Arabidopsis and DNA clones of rapeseed, DNA segment sequencing was performed with 25 clones which were found to be closely linked to the CMS restore gene, the boron efficiency gene (BE1), the Sclerotinia resistant QTLs and the characteristic of interspecific biomass heterosis in rapeseed respectively. Highly homologous sequences were found in Arabidopsis genome by searching the relative databases. According to the position of homologous sequences on the chromosomes of Arabidopsis, the 25 clones were integrated into the genetic map of Arabidopsis. Two markers flanking BE1 in B. napus were integrated into a narrow region at the short arm of chromosome 1 of Arabidopsis. One of EST clones, pA24, from this region was used as a probe to map B. napus and consequently been integrated in a position between the two flanking markers of BE1. This event increased the confident for one to map the important characteristics of cultured Brassica species via reciprocal comparative mapping between Arabidopsis and target Brassica crops.

Arabidopsis↗