Biomedical subjects

Jin-Yun Xie

Publications and source records attributed to Jin-Yun Xie.

3 recordsLinked to original sources

PubMed2005

DEPD: a novel database for differentially expressed proteins.

SUMMARY: The Differentially Expressed Protein Database was designed to store the output of comparative proteomics studies and provides a publicly available query and analysis platform for data mining. The database contains information about more than 3000 differentially expressed proteins (DEPs) manually extracted from the published literature, including relevant biological, experimental and methodological elements. Tools for visualization and functional analysis of DEPs are provided via a user-friendly webinterface. AVAILABILITY: http://protchem.hunnu.edu.cn/depd/.

Computational Biology↗

PubMed2003

[Identification of protein spots in silver-stained two-dimensional gels based on chemically assisted fragmentation by MALDI-TOF-PSD mass spectrometry].

Guanidination of the epsilon-amino group of lysine-terminated tryptic peptides,accomplished with O-methylisourea hydrogen sulfate, converted lysine into homoarginine residues, improving detection in MALDI-MS. Then tryptic peptides were labeled with sulfonic acid groups at the N-termini using 3-sulfopropionic acid NHS-ester chemistry. The derivatives enhanced post-source decay (PSD) fragmentation signals and produced a spectrum containing only y-ions. This facilitated de novo peptide sequencing, so it is an important contribution to unambiguous protein identification in proteome research. This method was successfully applied in nasopharyngeal carcinoma(NPC) proteome study.

Amino Acid Sequence↗

PubMed1997

Preliminary Study on the Primary Structure of Dragline Fibroin from the Spider Araneus ventrocosus.

The amino acid composition of the dragline fibroin from the spider fibroin of the spider Araneus ventrocosus was analysed and compared with that of the fibroins from different species of spiders. By means of partial acid hydrolysis and high performance liquid chromatography, several peptide fragments of the dragline fibroin were purified. The amino acid sequence analysis showed the sequences of these peptides to be different from that of the fibroin from the spider Nephila clavipes except for one common fragment with the sequence of GYGPG.

Journal Article↗