Biomedical subjects

Jing Liu

Publications and source records attributed to Jing Liu.

At least 271 records · Page 15Linked to original sources

PubMed2004

[The expression of transforming growth factor beta-1 in rat model of chronic obstructive pulmonary disease and the effects of early drugs intervention].

OBJECTIVE: To evaluate the expression of transforming growth factor beta-1(TGF-beta1) and the effects of early drugs intervention of chronic obstructive pulmonary disease(COPD) in rat model. METHODS: The COPD rat model (group B) was established by intratracheal instillation of lipopolysaccharide twice and daily exposure to cigarette smoking. Drug intervention groups received dongchongxiacao orally daily from the three days before the experiment (group C) and erythromycin by intraperitoneal injection since the third week (group D)and inhalation of budesonide since the forth week (group E). At the end of 10 weeks, all 40 rats including normal control (group A) were assessed for lung resistance (RL) and dynamic lung compliance (Cdyn). The expression of TGF-beta1 gene and protein were also observed by immunohistochemistry and semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR), respectively. RESULTS: The changes of pathology and pathophysiology in rat COPD model were similar to those of human COPD. There was a significant increase in the smooth muscle and collagen thickness in the airway wall of the group B in comparison with that of the group A. RL in group B was significantly higher than that in group A (P<0.01), while it was inhibited by early drugs intervention (P<0.01). Cdyn was decreased in group B as compared with that in group A, which was limited by erythromycin and budesonide intervention (P<0.01). The relative content for TGF-beta1 was significantly increased in the epithelial cells of the bronchi, endothelial cells of the pulmonary small vessel and alveolar macrophages of COPD group as compared with those of normal controls (P<0.01).The relative contents for TGF-beta1 in the epithelial of bronchi in group D and group E were significantly lower than that in group B, but not found in group C. There was no difference between group D and group E. There were statistical positive relationships between the RL and the relative content for TGF-beta1 in the bronchial epithelial cells, between the RL and the mRNA level of TGF-beta1 in the lung tissue (P<0.01 approximately 0.05). CONCLUSION: This rat COPD model could be helpful to obtain more information about airway remodeling. TGF-beta1 may play an important role during the process of airway remodeling, and could be influenced by early drugs intervention such as budesonide and erythromycin, which may imply their potency in the treatment of COPD. But there is not same phenomenon found in dongchongxiacao group.

Animals↗

PubMed2004

cDNA cloning, sequence analysis, and recombinant expression of akitonin beta, a C-type lectin-like protein from Agkistrodon acutus.

AIM: To clone the cDNA of a new member of snake venom C-type lectin-like proteins, to study its structure-function relationships and to achieve its recombinant production. METHODS: PCR primers were designed based on the homology and cDNA was amplified by RT-PCR using total RNA from snake venom gland as the template. The PCR products were cloned into the plasmid pGEM-T and sequenced. The deduced protein sequence was analyzed with some bioinformatic programs. A recombinant expression plasmid was constructed using pBAD-TOPO as vector and transformed into E.coli TOP10 competent cells. RESULTS: A novel cDNA sequence encoding akitonin beta was found and accepted by GenBank (accession number AF387100). Akitonin beta consists of a typical carbohydrate recognition domain (CRD) of C-type lectins, and it is homologous with other snake venom C-type lectin-like proteins. It was predicted to be a platelet antagonist. Upon induction with arabinose rAkitonin beta expressing in E coli was achieved at a high level (superior to 150 mg/L). The recombinant fusion protein exhibited inhibitory activities on rat platelet aggregation in vitro. CONCLUSION: A new member of snake venom C-type lectin-like proteins was discovered and characterized, and an efficient recombinant expression system was established for its production.

Agkistrodon↗

PubMed2004

[Pulmonary complications in haploidentical bone marrow transplantation].

UNLABELLED: To explore the occurrence patterns of pulmonary complications at different stages in haploidentical bone marrow transplantation, a series of clinical data as the onset time, etiology, management choices and prognosis in 18 patients with pulmonary disorders were summarized. The results showed that in 18 out of 70 patients after bone marrow transplantation occurred pulmonary complications which included pneumonia affected by bacteria (7 cases), fungus (5 cases) and cytomegalovirus (CMV, 4 cases), bronchiolitis obliterans organizing pneumonia (BOOP, 1 case), and idiopathic pneumonia syndrome (1 case), out of which 8 cases died. Fungal and CMV pneumonia occurred predominantly 2 to 3 months after transplantation, whereas bacterial pneumonia was observed in the duration of 3 to 12 months and 4 cases of them suffered from secondary fungal infections during treatment. BOOP and idiopathic pneumonia syndrome were diagnosed 12 months and 50 days after transplantation respectively. IN CONCLUSION: pulmonary complications were commonly seen in haploidentcal bone marrow transplantation, and fungal pneumonia might be the main cause that needs intensive management.

Adult↗

PubMed2004

[CD25 monoclonal antibody for GVHD prophylaxis in non-T-cell depleted haploidentical bone marrow transplantation for treatment of childhood leukemia].

OBJECTIVE: Allogeneic bone marrow transplantation (Allo-BMT) has improved long-term survival in children patients with refractory leukemia. For patients who do not have an HLA-identical sibling, the treatment option is limited. Using related mismatch donors or parental donors for Allo-BMT was at high risk for acute severe GVHD. The purpose of this study was to explore the effects of CD25 monoclonal antibody on reducing the incidence of severe acute GVHD in haploidentical bone marrow transplantation for the treatment of childhood leukemia. METHODS: Ten children with leukemia received haplotype Allo-BMT from HLA two or three loci mismatched related donors. Most patients were classified as in high risk category. The donors of patients were given G-CSF (Lenograstim Chugai) 250 microg/day for seven doses prior to marrow harvest. The non-T-cell depleted haploidentical bone marrow was infused. In addition to combination of CSA, MTX, ATG (Fresenius Hemocare, Germany) and mycophenolate mofetil (MMF) for GVHD prophylaxis, CD(25) monoclonal antibody (Simulect, Novartis Pharma Switzerland) was administered to prevent acute severe GVHD.A total of 40 mg Simulect was given in two doses of 20 mg each by 30 min intravenous infusion 2 h before transplantation and day 4 after transplantation. The outcomes were compared with those of 8 children patients with leukemia who received haploidentical bone marrow transplantation without CD(25) antibody for GVHD prophylaxis. RESULTS: All patients were engrafted and sustained full donor-type engraftment. 100% donors hematopoietic cells after transplantation was determined by cytogenetic evidence analysis. The median days of granulocyte exceeding 0.5 x 10(9)/L and pallets exceeding 20 x 10(9)/L were 19 and 22 days, respectively. Patients were monitored till up to days 100 for the sign of aGVHD. None developed the grade II-IV acute GVHD. However, the incidence of the grade II-IV acute GVHD in control group was 50% (P = 0.0147). Eight patients could be evaluated for chronic GVHD. All experienced chronic GVHD confined to the skin. The median follow-up duration was 12 months (range 9 - 24 months). Two patients died from transplant related mortality, one had patient relapsed disease and died. The remaining seven patients were alive in disease-free situation. CONCLUSION: The use of Simulect in haploidentical bone marrow transplantation for the treatment of children patients with leukemia is effective for preventing acute severe GVHD and may reduce transplant-related mortality.

Acute Disease↗

PubMed2004

[The effect of postoperative chemotherapy after esophagectomy on the survival rate to patients with original squamous cell carcinoma of esophagus].

OBJECTIVE: To study the prognostic factors affecting the survival rate after extended radical esophagectomy with three-field lymph node dissection for squamous cell carcinoma and the effect of postoperative chemotherapy. METHODS: Out of 126 patients with original squamous cell carcinoma of esophagus who accepted extended radical esophagectomy with three-field lymph node dissection from 1987 - 1992 in a hospital, 97 of them were included in this study. Data on the clinical/pathological characters and post surgery survival records of the subjects' were collected. Survival analysis methods included Kaplan-Meier, Log-rank test and Cox multivariable model and the effects of postoperative chemotherapy were analyzed for patients in early and late stages. RESULTS: There was no significant difference in clinical and pathological character between those patients only undergone surgery and patients accepting postoperative chemotherapy. The size of tumor, grade of differentiation of the tumor cells, infiltration deepness, with or without lymph node metastasis, expression of nm23 and EGFR and treatment after surgery etc. were correlated with the survival rate. For patients in early tumor stage, postoperative chemotherapy with cisplatin and 5-FU after surgery seemed to be a risk factor. For patients in late stage, postoperative chemotherapy with cisplatin and 5-FU after surgery did not seem to improve survival rate. CONCLUSION: It is imperative to study on the effect of adjuvant postoperative chemotherapy to patients, especially those at early stage with squamous cell carcinoma of esophagus. Doctors must be scrupulous when making decisions.

Adult↗

PubMed2004

[Detection of human telomerase reverse transcriptase mRNA of cells in pleural fluid by two-step in situ hybridization using digoxin-labeled oligonucleotide probes].

OBJECTIVE: To establish an method for detecting human telomerase reverse transcriptase (hTERT) mRNA of cells in pleural fluid by in situ hybridization (ISH), and to evaluate preliminarily the efficacy of this new method in recognizing neoplastic cells in the pleural fluid. METHODS: Fresh pleural fluid specimens were collected in 23 patients with pleural effusions and cell smears were prepared and after pretreatment, ISH between hTERT mRNA of the cells and digoxin-labelled oligonucleotide probes was performed. The signals of hTERT mRNA were detected by the sequential treatment with antigen, antibody, enzyme, and staining. With strict negative and positive controls, the experimental study was performed independent of clinical diagnosis and treatment. RESULTS: Positive results were seen in 9 cases of malignant pleural effusions, where dark staining of the cytoplasm and a few stained spots in the cell nuclei could be observed microscopically irrespective of the cytomorphological findings. Seven of the 9 cases had positive results of cytomorphological examination, in which neoplasmic cell with obvious morphological abnormalities were also spotted. In the other 2 cases where cytomorphological findings were negative but pleural biopsy confirmed the diagnosis of malignant pleural effusions, cytoplasm dark staining with stained spots in the cell nuclei were seen under microscope in spite of the absence of obvious abnormality in cell morphology. In contrast, the 14 cases with benign pleural effusions all had negative results, in which neither staining of the cells was identified microscopically, nor was evidently abnormal cell morphology. CONCLUSIONS: As a new method of clinical cytopathology, detection of hTERT mRNA of cells in the pleural fluid by ISH may provide a new means for cytomorphological examination for differential diagnosis between benign and malignant pleural effusions and for classification of the identified tumor cells.

DNA-Binding Proteins↗

PubMed2004

[Influence of mixed grafting of autologous and allogeneic microskin on burn wound healing in rats].

OBJECTIVE: To observe the influence of mixed grafting of autologous and allogeneic microskin on burn wound healing. METHODS: Autologous microskin grafting (expansion rate 5:1) was employed as control. Autologous microskin mixed with the allogeneic microskin with the thickness of 0.3 mm and 0.6 mm, respectively, were designated as experimental groups 1 and 2 (EP1 and EP2). The wound healing rate, wound contraction rate, and histological changes were observed on the 2nd, 3rd and 4th weeks after the grafting. RESULTS: The wound healing rate in two experimental groups (94.58 +/- 3.99)% in EP1, and (95.28 +/- 1.93)% in EP2 was significantly higher than that in the control group (88.28 +/- 6.85)% at the end of the 2nd week after the grafting (P < 0.05) The wound healing rate in experimental group 2 (94.55 +/- 3.47)% was obviously higher than that in control (88.51 +/- 5.59)% and experimental group 1 (89.51 +/- 4.70)% at the end of the 3rd week after grafting (P < 0.05). There was no obvious difference in wound healing rate among the three groups at the end of the 4th week after grafting. Obvious lymphocytic infiltration was observed by histological examination between epidermis and dermis in the two experimental groups at the end of the 2nd week after grafting. But there was no obvious difference among the three groups 4 weeks after grafting. CONCLUSION: The wound healing could be improved by mixed skin grafting with appropriate quantity of allogeneic and autologous microskin. Furthermore, the wound contraction could be ameliorated if the thickness of allogeneic dermis was increased in the mixed grafting even with the same proportion of allogeneic to autologous microskin.

Animals↗

PubMed2004

[Ultrasound criterion for the diagnosis of polycystic ovary syndrome: ovarian stroma/total area ratio].

OBJECTIVE: To evaluate the diagnostic value of ultrasound parameters of ovarian stroma area (S), total ovarian area (A) and S/A ratio for polycystic ovarian syndrome (PCOS). METHODS: The A, S and S/A ratio were determined by use of transvaginal pelvic ultrasound, and serum levels of follicle stimulating hormone (FSH), luteinizing hormone (LH) and testosterone (T) were assayed in 58 PCOS patients and 60 controls. Of 58 PCOS patients, 32 underwent hyperinsulinemic euglycemic clamp technique (HGCT). RESULTS: Patients with PCOS showed significantly higher ovarian S and S/A ratio when compared to the control groups (P = 0.045, P = 0.001, respectively). S/A ratio and S were significantly related to HGCT results and T. CONCLUSION: The S/A ratio and S are of value in diagnosis of PCOS.

Adult↗

PubMed2004

[Study on acute pancreatitis-associated lung injury induced by L-arginine in mice].

OBJECTIVE: To establish the model of acute pancreatitis (AP)-associated lung injury induced by L-arginine and study the role of TNF-alpha and ICAM-1 in the model mice. METHODS: For the establishment of the AP model, the mice were given the first injection (2 g/kg) of L-arginine intraperitoneally and then the second injection in the same way an hour later. At 12 h after the second injection, the animals' serum amylase activities were assayed by colorimetry, and the tumor necrosis factor-alpha (TNF-alpha) contents of their pancreatic tissue were determined by radioimmunoassay. At 24 h after the second injection, the histopathological changes in the animals' pancreatic and pulmonary tissues were observed. The pulmonary expression and cellular localization of intercellular adhesion molecule-1 (ICAM-1) were characterized by immunohistochemistry. RESULTS: The serum amylase activities, TNF-alpha content and ICAM-1 expression in lung tissue significantly increased in the AP group as compared with those in the control group. Histopathological examinations revealed that the acinar architecture was partially destroyed with necrosis, interstitial edema and inflammatory infiltration at 24 h after the second injection. CONCLUSION: TNF-alpha and ICAM-1 may play important roles in the mice with acute pancreatitis-associated lung injury.

Acute Disease↗

PubMed2004

[A prospective study of relationship between blood pressure and 10-year cardiovascular risk in a Chinese cohort aged 35-64 years].

OBJECTIVE: To provide epidemiological data for the revision of The National Guideline for Prevention and Treatment of Hypertension through the analysis of the association between blood pressure (BP) and the risk of cardiovascular diseases (CVD) in a cohort aged 35-64 years. METHODS: A prospective study was carried out in 11 provinces from 1992 to 2002. The association between BP and CVD (CHD and stroke) was analyzed in 31728 subjects aged 35 - 64 years. RESULTS: (1) Compared with BP < 110/75 mmHg, the risk of CVD increased 1 time (RR=2.09), 2 times (RR=3.23), and 10 times (RR=11.81) when BP was 120-129/80-84 mmHg, 140-149/90-94 mmHg, and > or = 180/110 mm Hg, respectively. (2) The risk of acute CHD for subjects with stage 2 hypertension was 2.3 times of those with optimal BP, whereas the risk of ischemic stroke and hemorrhagic stroke was 4.9 times and 11.7 times, respectively. (3) 36.1% of all CVDs, 44.0% of the acute stroke and 23.7% of the acute CHD were attributable to hypertension. (4) In each BP level, the 10-year risk of CVD increased with the increased number of other risk factors. CONCLUSIONS: The risk of CVD increases with BP continuously from BP of 110/75 mmHg. Therefore, the cutpoint of the diagnostic criteria for hypertension is arbitrary. Comprehensive intervention for multiple risk factor clustering should be strengthened to reduce the overall risk of CVD.

Adult↗

PubMed2004

[Determination of trace selenium in coal by microwave digestion and hydride generation ICP-AES].

A method for determining trace Se in coal by microwave digestion and hydride generation ICP-AES is reported. The digestion system of HNO3-HCl-HF was evaluated. The hydride generation occurred in 3 mol x L(-1) hydrochloric acid media with 1% sodium tetrahydroborate as a reduction reagent. The experimental conditions of microwave digestion, prereduction, and interferences were investigated. This method is simple and rapid. The treatment method was applicable to Se and gave results that were in good agreement with the certified values of the standard reference material. Testing results showed that the detection limit was 0.3 microg x L(-1) and RSD was below 5%.

Coal↗

PubMed2004

[Photolytic spectroscopy of Cs2 molecules].

Populations of the n2 L (nL = 7P, 6D) states of Cs atoms by photodissociation of Cs2 molecules through the use of the fixed frequence line 441.6 nm of a He-Cd laser radiation are produced. Combining ratios of atomic to molecular fluorescence at the Cs density range between 1 and 9 x 10(15) cm(-3), the ratios of collisional rate coefficient to predissociation rates are estimated to be 2.9 x 10(-17) cm3 and 7.4 x 10(-18) cm3, respectively. The branching ratios are defined as the ratios of nL(J) to nL(J) fluorescence. The ratios of the dissociation rates produced in n2 L fine-structure states are 0.53 and 0.43, respectively. Fine-structure changing cross section has been measured for far wing excitation, the result is consistent with cross section obtained from other excitation of the n2 L states. The cross section for excitation transfer out of the 6D doublet [i.e., Cs(6D) + Cs(6S) --> states other than Cs(6D)] is 1.9 x 10(-14) cm2.

Cesium↗

PubMed2004

[Determination of trace mercury in coal by microwave digestion and cold vapor atomic fluorescence spectrometry].

A method of determining trace Hg in coal by microwave digestion and cold vapor atomic fluorescence spectrometry is reported. The digestion system of HNO3-HCl-HF was evaluated. The experimental conditions of microwave digestion, including pressure, temperature, power and time of digestion were evaluated. The operating conditions such as flow rate of carrier gas, flow rate of shield gas and negative high voltage, and interference were also investigated. Testing results show that the detection limit is 0.03 ng x mL(-1) and RSD (n = 7) is below 5%. This method is simple and rapid. The treatment method was applicable to determining trace Hg in coal and gave results that were in good agreement with the certified values of the standard reference material.

Coal↗

PubMed2004

[Effects of the glucosidorum tripterygll totorum on the proliferation and apoptosis of cell line HL-60 in human acute promyelocytic leukemia].

OBJECTIVE: To determine the effects of the glucosidorum tripterygll totorum on the proliferation inhibition and apoptosis induction of cell line HL-60 in human acute promyelocytic leukemia. METHODS: The survival rates of cells were tested by MTT assay, fluorescence stain, flow cytometry test, and DNA fragmentation. RESULTS: Glucosidorum tripterygll totorum could notably reduce the survival rate of HL-60 cells, and inhibit the proliferation of tumor cells. The 50% inhibitory concentration (IC50) was 5.0 microg/ml and it arrested the cell cycle at G2/M phase (P < 0. 05). The typical apoptosis morphologic cells were seen, the rates of apoptosis cells were increased (P < 0.05), and DNA ladder appeared in the experiment group. CONCLUSION: Glucosidorum tripterygll totorum could notably inhibit the proliferation and induce the apoptosis of HL-60 cells.

Antineoplastic Agents, Phytogenic↗

PubMed2004

[Blood transfusion during orthotopic liver transplantation].

OBJECTIVE: To summarize the experience of blood transfusion during orthotopic liver transplantation (OLT). METHODS: The clinic data and blood transfusion of 28 patients during OLT were analyzed. RESULTS: The total blood volume of transfusion was 9 517 +/- 7 680 ml averagely for the 28 patients in general during OLT, including that of an infant (920 ml), massive blood transfusion in 2 cases (28740 ml and 38580 ml respectively). The transfusion volume of patients with anemia or/and thrombcytopenia before the operation was not more than that of patients with normal blood routine. CONCLUSION: In general, 5 000 - 10 000 ml is reserved for receptor of OLT before the operation. We should take measures to deal with massive blood transfusion. The preparative blood volume for receptor of OLT can not depend on the result of blood routine.

Adolescent↗

PubMed2003

A novel mutation in GJA3 (connexin46) for autosomal dominant congenital nuclear pulverulent cataract.

PURPOSE: Autosomal dominant congenital cataract (ADCC) is a clinically and genetically heterogeneous lens disorder that usually presents as a sight-threatening trait in childhood. Here, we described a six-generation Chinese family presenting with morphologically homogeneous "nuclear pulverulent" cataracts. METHODS: A whole genome scan was performed with polymorphic microsatellites in the Human MapPairs marker set, with special attention paid to known ADCC loci. Then we screened for mutations by direct sequencing. RESULTS: A significantly positive two-point LOD score was obtained at marker D13S175(Zmax=7.83, theta=0). Haplotype analysis indicated this disease was located at 13q11, close to GJA3. Upon screening for mutations in GJA3 in this family, we found a novel transition mutation (TTC->TTA) resulting in a Phenylalanine-Leucine substitution at the highly conserved codon 32 of the GJA3 protein. This mutation segregated with the affected members of the family. CONCLUSIONS: This finding is the first report of a mutation in the first transmembrane region of GJA3. Our study further confirmed that GJA3 plays a vital role in the maintenance of human lens transparency.

Amino Acid Sequence↗

PubMed2003

Mechanism of enhanced cardiac function in mice with hypertrophy induced by overexpressed Akt.

Transgenic mice with cardiac-specific overexpression of active Akt (TG) not only exhibit hypertrophy but also show enhanced left ventricular (LV) function. In 3-4-month-old TG, heart/body weight was increased by 60% and LV ejection fraction was elevated (84 +/- 2%, p < 0.01) compared with nontransgenic littermates (wild type (WT)) (73 +/- 1%). An increase in isolated ventricular myocyte contractile function (% contraction) in TG compared with WT (6.1 +/- 0.2 versus 3.5 +/- 0.2%, p < 0.01) was associated with increased Fura-2 Ca2+ transients (396 +/- 50 versus 250 +/- 24 nmol/liter, p < 0.05). The rate of relaxation (+dL/dt) was also enhanced in TG (214 +/- 15 versus 98 +/- 18 microm/s, p < 0.01). L-type Ca2+ current (ICa) density was increased in TG compared with WT (-9.0 +/- 0.3 versus 7.2 +/- 0.3 pA/pF, p < 0.01). Sarcoplasmic reticulum Ca2+ ATPase 2a (SERCA2a) protein levels were increased (p < 0.05) by 6.6-fold in TG, which could be recapitulated in vitro by adenovirus-mediated overexpression of Akt in cultured adult ventricular myocytes. Conversely, inhibiting SERCA with either ryanodine or thapsigargin affected myocyte contraction and relaxation and Ca2+ channel kinetics more in TG than in WT. Thus, myocytes from mice with overexpressed Akt demonstrated enhanced contractility and relaxation, Fura-2 Ca2+ transients, and Ca2+ channel currents. Furthermore, increased protein expression of SERCA2a plays an important role in mediating enhanced LV function by Akt. Up-regulation of SERCA2a expression and enhanced LV myocyte contraction and relaxation in Akt-induced hypertrophy is opposite to the down-regulation of SERCA2a and reduced contractile function observed in many other forms of LV hypertrophy.

Adenoviridae↗

PubMed2003

Glycation end-product cross-link breaker reduces collagen and improves cardiac function in aging diabetic heart.

Aging and diabetes mellitus (DM) both affect the structure and function of the myocardium, resulting in increased collagen in the heart and reduced cardiac function. As part of this process, hyperglycemia is a stimulus for the production of advanced glycation end products (AGEs), which covalently modify proteins and impair cell function. The goals of this study were first to examine the combined effects of aging and DM on hemodynamics and collagen types in the myocardium in 12 dogs, 9-12 yr old, and second to examine the effects of the AGE cross-link breaker phenyl-4,5-dimethylthazolium chloride (ALT-711) on myocardial collagen protein content, aortic stiffness, and left ventricular (LV) function in the aged diabetic heart. The alloxan model of DM was utilized to study the effects of DM on the aging heart. DM induced in the aging heart decreased LV systolic function (LV ejection fraction fell by 25%), increased aortic stiffness, and increased collagen type I and type III protein content. ALT-711 restored LV ejection fraction, reduced aortic stiffness and LV mass with no reduction in blood glucose level (199 +/- 17 mg/dl), and reversed the upregulation of collagen type I and type III. Myocardial LV collagen solubility (%) increased significantly after treatment with ALT-711. These data suggest that an AGE cross-link breaker may have a therapeutic role in aged patients with DM.

Aging↗