Biomedical subjects

Jinsook Ryu

Publications and source records attributed to Jinsook Ryu.

3 recordsLinked to original sources

PubMed2005

Thermodynamic prediction of polymer retention in temperature-programmed HPLC.

Temperature programming has been used increasingly in liquid chromatography in recent years. In particular, temperature gradient elution has shown great potential in the analysis of complex polymers. In this study, the polymer retention behavior in temperature gradient interaction chromatography is investigated based on thermodynamic consideration of the retention factor. The polymer retention predicted by the model calculation is in good agreement with the experimental results, and the model allows devising a temperature program for designed retention behaviors such as a linear dependence of retention volume on log(molecular weight) of polymers. In addition, the migration behavior of polymeric solute along the separation column can be simulated, which shows strong molecular weight dependence. The migration behavior is also confirmed experimentally by employing different length columns or delayed injection.

Journal Article↗

PubMed2005

Rapid molecular weight analysis of polymers by temperature gradient interaction chromatography.

Temperature gradient interaction chromatography (TGIC) has been established as a high-resolution technique for the characterization of synthetic polymers. So far, most of the TGIC investigations focused on the high-resolution analysis and little effort has been made on the reduction of the analysis time. In this study, we examined the effect of the column heating rate, the eluent flow rate, and the column length on the TGIC analysis time. We found that the heating rate is the most important experimental parameter to control the TGIC retention time. With a C18 silica column (50 mm x 4.6mm I.D.), a set of PS standards of wide molecular weight range (5 - 648 kg/mol) could be separated within 4 min at a heating rate of 8 degrees C/min.

Molecular Weight↗

PubMed2002

Temperature gradient interaction chromatography and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis of air terminated polystyryllithium.

The reaction products of polystyryllithium with air were characterized by size-exclusion chromatography, temperature gradient interaction chromatography and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Polystyryllithium was prepared by anionic polymerization of styrene initiated with sec-butyllithium in cyclohexane under an Ar atmosphere. It was confirmed that polystyryl ketone, polystyryl alcohol, and directly coupled polystyrene were the major products in addition to the normally terminated polystyrene, which is consistent with the results in the literature. We could also identify the presence of methoxy and carboxylic acid end capped polystyrenes as well as dipolystyryl ether as minor products. Among the minor products, dipolystyryl ether has not been reported yet.

Air↗